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SMAD3 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179069

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that… More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Smad3, Monoclonal Antibody
Full Product Name
Anti-Smad3 Rabbit Monoclonal Antibody
Product Gene Name
anti-Smad3 antibody
Product Synonym Names
Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
48081 MW
OMIM
U68019 mRNA
3D Structure
ModBase 3D Structure for P84022
Immunogen
A synthesized peptide derived from human Smad3
Subcellular Localization
Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1. Co-localizes with LEMD3 at the nucleus inner membrane. MAPK-mediated phosphorylation appears to have no effect on nuclear import. PDPK1 prevents its nuclear translocation in response to TGF-beta.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-Smad3 antibody

Signal Transduction; Signaling Pathway; Nuclear Signaling; smads; Epigenetics and Nuclear Signaling; Nuclear Signaling Pathways; Stem Cells; TGF Beta; Cytoplasmic; Cancer; smad family; Cancer Metabolism; Response to Hypoxia; Metabolism; Pathways and Proce
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-Smad3 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-Smad3 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
FC/FACS: 1:50

Western Blot (WB) of anti-Smad3 antibody

Western blot analysis of Smad3 expression in (1) Jurkat cell lysate; (2) Rat liver lysate (MBS179069).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SMAD3
Rabbit Anti-SMAD3 Monoclonal Antibody – Cat# MBS179069

Immunohistochemistry (IHC) of anti-Smad3 antibody

Immunohistochemical analysis of paraffin-embedded mouse kidney, using Smad3 Antibody(MBS179069)
SMAD3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SMAD3 Antibody (MBS179069)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-SMAD3 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS179069

Other Notes

Small volumes of anti-Smad3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Smad3 purchase

MBS179069 is an antibody targeting Smad3. Also known as Anti-Smad3 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Smad3. It may not necessarily be applicable to this product.

NCBI GI #
223029440
NCBI GeneID
4088
NCBI Accession #
NP_001138574.1
UniProt Primary Accession #
P84022
UniProt Secondary Accession #
O09064; O09144; O14510; O35273; Q92940; Q93002; A8K4B6; B7Z4Z5; B7Z6M9; B7Z9Q2; F5H383
UniProt Related Accession #
P84022
GenBank Nucleotide #
NM_001145102.1
NCBI Official Full Name
mothers against decapentaplegic homolog 3 isoform 2
NCBI Official Synonym Full Names
SMAD family member 3
NCBI Official Symbol
SMAD3
NCBI Official Synonym Symbols
LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
UniProt Protein Name
Mothers against decapentaplegic homolog 3
UniProt Synonym Protein Names
JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
Protein Family
Mothers against decapentaplegic
UniProt Gene Name
SMAD3
UniProt Synonym Gene Names
MADH3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD 3; Smad3; hSMAD3
NCBI Protein Information
mothers against decapentaplegic homolog 3

NCBI Summary for Smad3

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]
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UniProt Comments for Smad3

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
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Research Articles on Smad3

1. Authors were able to confirm expression of SMAD3 in intact and degraded cartilage of the knee and hip. Our findings provide the first systematic evaluation of pleiotropy between OA and BMD, highlight genes with biological relevance to both traits, and establish a robust new OA genetic risk locus at SMAD3.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Smad3 antibody

 Reference Product  PubMed Publications
 SMAD2 antibody  >1384 publications with Smad3 and SMAD2
 SMAD4 antibody  >729 publications with Smad3 and SMAD4
 JUN antibody  >407 publications with Smad3 and JUN
 SMAD1 antibody  >169 publications with Smad3 and SMAD1
 MYC antibody  >88 publications with Smad3 and MYC
 CTNNB1 antibody  >85 publications with Smad3 and CTNNB1
 SP1 antibody  >72 publications with Smad3 and SP1
 SKI antibody  >63 publications with Smad3 and SKI
 MAPK1 antibody  >44 publications with Smad3 and MAPK1
 RUNX2 antibody  >37 publications with Smad3 and RUNX2

Pathways associated with anti-Smad3 antibody

 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram

Diseases associated with anti-Smad3 antibody

 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >674 publications with Smad3 and Fibrosis
 Neoplasms Antibodies  >581 publications with Smad3 and Neoplasms
 Disease Models, Animal Antibodies  >245 publications with Smad3 and Disease Models, Animal
 Inflammation Antibodies  >244 publications with Smad3 and Inflammation
 Liver Diseases Antibodies  >175 publications with Smad3 and Liver Diseases
 Kidney Diseases Antibodies  >152 publications with Smad3 and Kidney Diseases
 Cardiovascular Diseases Antibodies  >145 publications with Smad3 and Cardiovascular Diseases
 Breast Neoplasms Antibodies  >100 publications with Smad3 and Breast Neoplasms
 Necrosis Antibodies  >92 publications with Smad3 and Necrosis
 Liver Cirrhosis Antibodies  >91 publications with Smad3 and Liver Cirrhosis

Organs/Tissues associated with anti-Smad3 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >452 publications with Smad3 and Muscle
 Lung Antibodies  >276 publications with Smad3 and Lung
 Skin Antibodies  >195 publications with Smad3 and Skin
 Heart Antibodies  >143 publications with Smad3 and Heart
 Embryonic Tissue Antibodies  >137 publications with Smad3 and Embryonic Tissue
 Intestine Antibodies  >56 publications with Smad3 and Intestine
 Thyroid Antibodies  >28 publications with Smad3 and Thyroid
 Ascites Antibodies  >1 publications with Smad3 and Ascites
 Uncharacterized Tissue Antibodies  >1 publications with Smad3 and Uncharacterized Tissue
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