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RUNX1/RUNX2/RUNX3 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179090

CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in… More details

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Product Overview

Online Datasheet in PDF
Product Name
RUNX1/RUNX2/RUNX3, Monoclonal Antibody
Full Product Name
Anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal Antibody
Product Gene Name
anti-RUNX1/RUNX2/RUNX3 antibody
Product Synonym Names
Runt-related transcription factor 1; Acute myeloid leukemia 1 protein; Core-binding factor subunit alpha-2; CBF-alpha-2; Oncogene AML-1; Polyomavirus enhancer-binding protein 2 alpha B subunit; PEA2-alpha B; PEBP2-alpha B; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit; RUNX1; AML1; CBFA2
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
48737 MW
OMIM
L34598 mRNA
3D Structure
ModBase 3D Structure for Q01196
Immunogen
A synthesized peptide derived from human RUNX1/RUNX2/RUNX3
Subcellular Localization
Nucleus.
Tissue Specificity
Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-RUNX1/RUNX2/RUNX3 antibody

Neuroscience; Neurology Process; Neurogenesis; Epigenetics and Nuclear Signaling; Transcription; Other Factors; Stem Cells; Hematopoietic Progenitors; Intracellular molecules; Transcription Factors; chromatin Binding Proteins; dna/rna binding; Mesenchymal
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-RUNX1/RUNX2/RUNX3 antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-RUNX1/RUNX2/RUNX3 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50

Western Blot (WB) of anti-RUNX1/RUNX2/RUNX3 antibody

Western blot analysis of RUNX1/RUNX2/RUNX3 expression in MOLT4 cell lysate (MBS179090).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUNX1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RUNX1
Rabbit Anti-RUNX1/RUNX2/RUNX3 Monoclonal Antibody – Cat# MBS179090

Other Notes

Small volumes of anti-RUNX1/RUNX2/RUNX3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RUNX1/RUNX2/RUNX3 purchase

MBS179090 is an antibody targeting RUNX1/RUNX2/RUNX3. Also known as Anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RUNX1/RUNX2/RUNX3. It may not necessarily be applicable to this product.

NCBI GI #
49574546
NCBI GeneID
861
NCBI Accession #
NP_001001890.1
UniProt Primary Accession #
Q01196
UniProt Secondary Accession #
O60472; O60473; O76047; O76089; Q13081; Q13755; Q13756; Q13757; A8MV94; B2RMS4; D3DSG1
UniProt Related Accession #
Q01196
GenBank Nucleotide #
NM_001001890.2
NCBI Official Full Name
runt-related transcription factor 1 isoform AML1b
NCBI Official Synonym Full Names
runt related transcription factor 1
NCBI Official Symbol
RUNX1
NCBI Official Synonym Symbols
AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; CBF2alpha; AML1-EVI-1; PEBP2alpha
UniProt Protein Name
Runt-related transcription factor 1
UniProt Synonym Protein Names
Acute myeloid leukemia 1 protein; Core-binding factor subunit alpha-2; CBF-alpha-2; Oncogene AML-1; Polyomavirus enhancer-binding protein 2 alpha B subunit; PEA2-alpha B; PEBP2-alpha B; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit
UniProt Gene Name
RUNX1
UniProt Synonym Gene Names
AML1; CBFA2; CBF-alpha-2; PEA2-alpha B; PEBP2-alpha B
NCBI Protein Information
runt-related transcription factor 1

NCBI Summary for RUNX1/RUNX2/RUNX3

Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for RUNX1/RUNX2/RUNX3

CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B-dependent transcriptional activation. Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down-regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells (PubMed:17377532). Positively regulates the expression of RORC in T-helper 17 cells ().
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Research Articles on RUNX1/RUNX2/RUNX3

1. The here presented transcriptomic subgroup-based approach unified the gene expression profiles of RUNX1-CBFA2T3 and RUNX1-RUNX1T1 acute myeloid leukemia.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RUNX1/RUNX2/RUNX3 antibody

 Reference Product  PubMed Publications
 JUN antibody  >206 publications with RUNX1/RUNX2/RUNX3 and JUN
 CBFB antibody  >75 publications with RUNX1/RUNX2/RUNX3 and CBFB
 CEBPA antibody  >57 publications with RUNX1/RUNX2/RUNX3 and CEBPA
 PAX5 antibody  >20 publications with RUNX1/RUNX2/RUNX3 and PAX5
 FOXP3 antibody  >18 publications with RUNX1/RUNX2/RUNX3 and FOXP3
 CCND1 antibody  >12 publications with RUNX1/RUNX2/RUNX3 and CCND1
 FOS antibody  >9 publications with RUNX1/RUNX2/RUNX3 and FOS
 EP300 antibody  >8 publications with RUNX1/RUNX2/RUNX3 and EP300
 CBFA2T2 antibody  >7 publications with RUNX1/RUNX2/RUNX3 and CBFA2T2
 KAT6A antibody  >7 publications with RUNX1/RUNX2/RUNX3 and KAT6A

Pathways associated with anti-RUNX1/RUNX2/RUNX3 antibody

 Products by Pathway  Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Chronic Myeloid Leukemia Pathway antibodies  Chronic Myeloid Leukemia Pathway Diagram
 Chronic Myeloid Leukemia Pathway antibodies  Chronic Myeloid Leukemia Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram

Diseases associated with anti-RUNX1/RUNX2/RUNX3 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1425 publications with RUNX1/RUNX2/RUNX3 and Neoplasms
 Leukemia, Myeloid, Acute Antibodies  >927 publications with RUNX1/RUNX2/RUNX3 and Leukemia, Myeloid, Acute
 Lymphoma Antibodies  >483 publications with RUNX1/RUNX2/RUNX3 and Lymphoma
 Precursor B-Cell Lymphoblastic Leukemia-Lymphoma Antibodies  >117 publications with RUNX1/RUNX2/RUNX3 and Precursor B-Cell Lymphoblastic Leukemia-Lymphoma
 Nervous System Diseases Antibodies  >80 publications with RUNX1/RUNX2/RUNX3 and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >72 publications with RUNX1/RUNX2/RUNX3 and Neoplasms, Experimental
 Disease Models, Animal Antibodies  >66 publications with RUNX1/RUNX2/RUNX3 and Disease Models, Animal
 Carcinoma Antibodies  >36 publications with RUNX1/RUNX2/RUNX3 and Carcinoma
 Anemia Antibodies  >35 publications with RUNX1/RUNX2/RUNX3 and Anemia
 Brain Diseases Antibodies  >22 publications with RUNX1/RUNX2/RUNX3 and Brain Diseases

Organs/Tissues associated with anti-RUNX1/RUNX2/RUNX3 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Marrow Antibodies  >373 publications with RUNX1/RUNX2/RUNX3 and Bone Marrow
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