PD-L1 Antibody – Rabbit Monoclonal

Figure 1. Western blot analysis of PD-L1 using anti-PD-L1 antibody (MBS179113). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.

Lane 1: human Ramos whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human THP-1 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat thymus tissue lysates,
Lane 6: mouse thymus tissue lysates.

After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- PD-L1 antigen affinity purified monoclonal antibody (Catalog # MBS179113) at 1:100 overnight at 4 degree C, then washed withTBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for PD-L1 at approximately 40 kDa. The expected band size for PD-L1 is at 33 kDa.

Immunohistochemical analysis of paraffin-embedded human thymoma, using PD-L1 (CD274) Antibody.