Rabbit Anti-CDK4 Monoclonal Antibody – Cat# MBS179151

Western blot analysis of CDK4 expression in HeLa cell lysate (MBS179151).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK4 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDK4

CDK4 Antibody – Rabbit Monoclonal

Name: CDK4 Antibody; Monoclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179151
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179151

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CDK4, Monoclonal Antibody

Full Product Name

Anti-CDK4 Rabbit Monoclonal Antibody

Product Gene Name

anti-CDK4 antibody

Product Synonym Names

Cyclin-dependent kinase 4; 2.7.11.22; Cell division protein kinase 4; PSK-J3; CDK4

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

33730 MW

OMIM

M14505 mRNA

3D Structure

ModBase 3D Structure for P11802

Immunogen

A synthesized peptide derived from human CDK4

Subcellular Localization

Cytoplasm. Nucleus. Membrane. Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-CDK4 antibody

Cell Biology; Cell Cycle; Kinases/Phosphatases; cdks; Epigenetics and Nuclear Signaling; Cancer; isotype/loading controls; cdk4

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-CDK4 antibody

Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)

Application Notes for anti-CDK4 antibody

WB: 1:1000-1:2000
ICC/IF: 1:50-1:200
IP: 1:50

Other Notes

Small volumes of anti-CDK4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CDK4 purchase

MBS179151 is an antibody targeting CDK4. Also known as Anti-CDK4 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CDK4. It may not necessarily be applicable to this product.

NCBI GI #

4502735

NCBI GeneID

1019

NCBI Accession #

NP_000066.1

UniProt Primary Accession #

P11802

UniProt Secondary Accession #

O00576; Q6FG61; B2R9A0; B4DNF9

UniProt Related Accession #

P11802

GenBank Nucleotide #

NM_000075.3

NCBI Official Full Name

cyclin-dependent kinase 4

NCBI Official Synonym Full Names

cyclin dependent kinase 4

NCBI Official Symbol

CDK4

NCBI Official Synonym Symbols

CMM3; PSK-J3

UniProt Protein Name

Cyclin-dependent kinase 4

UniProt Synonym Protein Names

Cell division protein kinase 4; PSK-J3

Protein Family

Cyclin-dependent kinase

UniProt Gene Name

CDK4

NCBI Protein Information

cyclin-dependent kinase 4

NCBI Summary for CDK4

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq, Jul 2008]

UniProt Comments for CDK4

Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

Research Articles on CDK4

1. Ribociclib (an oral, highly specific cyclin-dependent kinase 4/6 inhibitor) inhibits tumor growth.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CDK4 antibody

Reference Product	PubMed Publications
CCND1 antibody	>1837 publications with CDK4 and CCND1
CDKN2A antibody	>953 publications with CDK4 and CDKN2A
CDKN1A antibody	>724 publications with CDK4 and CDKN1A
CDKN1B antibody	>649 publications with CDK4 and CDKN1B
CCND3 antibody	>147 publications with CDK4 and CCND3
CCND2 antibody	>140 publications with CDK4 and CCND2
RB1 antibody	>67 publications with CDK4 and RB1
CDC37 antibody	>28 publications with CDK4 and CDC37
CCNA2 antibody	>25 publications with CDK4 and CCNA2
CCNE1 antibody	>25 publications with CDK4 and CCNE1

Pathways associated with anti-CDK4 antibody

Products by Pathway	Pathway Diagram
ATF-2 Transcription Factor Network Pathway antibodies	ATF-2 Transcription Factor Network Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram
Bladder Cancer Pathway antibodies	Bladder Cancer Pathway Diagram
Bladder Cancer Pathway antibodies	Bladder Cancer Pathway Diagram
Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies	Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram

Diseases associated with anti-CDK4 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>2279 publications with CDK4 and Neoplasms
Carcinoma Antibodies	>701 publications with CDK4 and Carcinoma
Breast Neoplasms Antibodies	>270 publications with CDK4 and Breast Neoplasms
Adenocarcinoma Antibodies	>265 publications with CDK4 and Adenocarcinoma
Cell Transformation, Neoplastic Antibodies	>225 publications with CDK4 and Cell Transformation, Neoplastic
Nervous System Diseases Antibodies	>207 publications with CDK4 and Nervous System Diseases
Neoplasms, Experimental Antibodies	>200 publications with CDK4 and Neoplasms, Experimental
Skin Neoplasms Antibodies	>196 publications with CDK4 and Skin Neoplasms
Lung Neoplasms Antibodies	>151 publications with CDK4 and Lung Neoplasms
Liver Diseases Antibodies	>147 publications with CDK4 and Liver Diseases

Organs/Tissues associated with anti-CDK4 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>381 publications with CDK4 and Blood
Skin Antibodies	>259 publications with CDK4 and Skin
Lung Antibodies	>251 publications with CDK4 and Lung
Muscle Antibodies	>251 publications with CDK4 and Muscle
Liver Antibodies	>245 publications with CDK4 and Liver
Brain Antibodies	>238 publications with CDK4 and Brain
Vascular Antibodies	>196 publications with CDK4 and Vascular
Bone Antibodies	>149 publications with CDK4 and Bone
Prostate Antibodies	>143 publications with CDK4 and Prostate
Kidney Antibodies	>97 publications with CDK4 and Kidney