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PRC1 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179153

Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle… More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PRC1, Monoclonal Antibody
Full Product Name
Anti-PRC1 Rabbit Monoclonal Antibody
Product Gene Name
anti-PRC1 antibody
Product Synonym Names
Protein regulator of cytokinesis 1; PRC1
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
71607 MW
OMIM
AF044588 mRNA
3D Structure
ModBase 3D Structure for O43663
Immunogen
A synthesized peptide derived from human PRC1
Subcellular Localization
Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Midbody. Colocalized with KIF20B in the nucleus of bladder carcinoma cells at the interphase. Colocalized with KIF20B in bladder carcinoma cells at prophase, metaphase, early anaphase, at the midzone in late anaphase and at the contractile ring in telophase (PubMed: 17409436). Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis.
Tissue Specificity
Overexpressed in bladder cancer cells (PubMed: 17409436).
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-PRC1 antibody

Cell Biology; Cell Cycle; Cell Division; cytokinesis
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-PRC1 antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-PRC1 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50
FC/FACS: 1:50

Western Blot (WB) of anti-PRC1 antibody

Western blot analysis of PRC1 expression in HeLa cell lysate (MBS179153).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRC1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRC1
Rabbit Anti-PRC1 Monoclonal Antibody – Cat# MBS179153

Other Notes

Small volumes of anti-PRC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PRC1 purchase

MBS179153 is an antibody targeting PRC1. Also known as Anti-PRC1 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PRC1. It may not necessarily be applicable to this product.

NCBI GI #
389886545
NCBI GeneID
9055
NCBI Accession #
NP_001254509.1
UniProt Primary Accession #
O43663
UniProt Secondary Accession #
Q9BSB6; A6NC44; B4DLR1; H9KV59
UniProt Related Accession #
O43663
GenBank Nucleotide #
NM_001267580.1
NCBI Official Full Name
protein regulator of cytokinesis 1 isoform 4
NCBI Official Synonym Full Names
protein regulator of cytokinesis 1
NCBI Official Symbol
PRC1
NCBI Official Synonym Symbols
ASE1
UniProt Protein Name
Protein regulator of cytokinesis 1
Protein Family
Protein regulator of cytokinesis
UniProt Gene Name
PRC1
NCBI Protein Information
protein regulator of cytokinesis 1

NCBI Summary for PRC1

This gene encodes a protein that is involved in cytokinesis. The protein is present at high levels during the S and G2/M phases of mitosis but its levels drop dramatically when the cell exits mitosis and enters the G1 phase. It is located in the nucleus during interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). It is necessary for polarizing parallel microtubules and concentrating the factors responsible for contractile ring assembly. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]
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UniProt Comments for PRC1

Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression (PubMed:17409436).
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Research Articles on PRC1

1. study reveals that UbE2E1 is an in vivo E2 for the PRC1 ligase complex and thus plays an important role in the regulation of H2A Lys-119 monoubiquitination

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PRC1 antibody

 Reference Product  PubMed Publications
 CBX2 antibody  >50 publications with PRC1 and CBX2
 PLK1 antibody  >13 publications with PRC1 and PLK1
 CDK1 antibody  >13 publications with PRC1 and CDK1
 KIF4A antibody  >8 publications with PRC1 and KIF4A
 CDK2 antibody  >7 publications with PRC1 and CDK2
 KIF14 antibody  >7 publications with PRC1 and KIF14
 KIF23 antibody  >6 publications with PRC1 and KIF23
 CENPE antibody  >1 publications with PRC1 and CENPE
 RACGAP1 antibody  >1 publications with PRC1 and RACGAP1

Pathways associated with anti-PRC1 antibody

 Products by Pathway  Pathway Diagram
 RHO GTPase Effectors Pathway antibodies  RHO GTPase Effectors Pathway Diagram
 RHO GTPases Activate CIT Pathway antibodies  RHO GTPases Activate CIT Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By Rho GTPases Pathway antibodies  Signaling By Rho GTPases Pathway Diagram

Diseases associated with anti-PRC1 antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >250 publications with PRC1 and Carcinoma
 Cell Transformation, Neoplastic Antibodies  >115 publications with PRC1 and Cell Transformation, Neoplastic
 Adenocarcinoma Antibodies  >104 publications with PRC1 and Adenocarcinoma
 Breast Neoplasms Antibodies  >89 publications with PRC1 and Breast Neoplasms
 Neoplasm Metastasis Antibodies  >69 publications with PRC1 and Neoplasm Metastasis
 Nervous System Diseases Antibodies  >65 publications with PRC1 and Nervous System Diseases
 Lung Neoplasms Antibodies  >54 publications with PRC1 and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >52 publications with PRC1 and Neoplasms, Experimental
 Disease Models, Animal Antibodies  >50 publications with PRC1 and Disease Models, Animal
 Lung Diseases Antibodies  >49 publications with PRC1 and Lung Diseases

Organs/Tissues associated with anti-PRC1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >136 publications with PRC1 and Blood
 Brain Antibodies  >128 publications with PRC1 and Brain
 Bone Antibodies  >103 publications with PRC1 and Bone
 Lung Antibodies  >77 publications with PRC1 and Lung
 Liver Antibodies  >75 publications with PRC1 and Liver
 Embryonic Tissue Antibodies  >70 publications with PRC1 and Embryonic Tissue
 Bone Marrow Antibodies  >53 publications with PRC1 and Bone Marrow
 Eye Antibodies  >49 publications with PRC1 and Eye
 Lymph Node Antibodies  >47 publications with PRC1 and Lymph Node
 Lymph Antibodies  >46 publications with PRC1 and Lymph
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