Rabbit Anti-C3 Monoclonal Antibody – Cat# MBS179158

Western blot analysis of C3 expression in HepG2 cell lysate (MBS179158).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (stacking gel) / 90V (resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C3 monoclonal antibody (MBS179158) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (please inquire) with Tanon 5200 system. A specific band was detected for C3.

C3 Antibody – Rabbit Monoclonal

Name: C3 Antibody; Monoclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179158
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179158

Anti-C3 Rabbit Monoclonal Antibody tested for Flow Cytometry, IF, ICC, WB in Human More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

C3/Complement C3, Monoclonal Antibody

Full Product Name

Anti-C3/Complement C3 Rabbit Monoclonal Antibody

Product Gene Name

anti-C3 antibody

Clonality

Monoclonal

Isotype

IgG

Clone Number

AOHE-3

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid; Phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Purity/Purification

Affinity-chromatography

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. (lot specific)

Preparation and Storage

Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for long term. Avoid repeated freezing and thawing.

OMIM

K02765 mRNA

3D Structure

ModBase 3D Structure for P01024

Immunogen

A synthesized peptide derived from human C3.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-C3 antibody

Flow Cytometry (FC), Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)

Application Notes for anti-C3 antibody

Suggested Dilutions:
WB 1:500-1:2000
ICC/IF 1:50-1:200
FC: 1:20

Other Notes

Small volumes of anti-C3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for C3 purchase

MBS179158 is an antibody targeting C3. Also known as Anti-C3/Complement C3 Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for C3. It may not necessarily be applicable to this product.

NCBI GI #

115298678

NCBI GeneID

718

NCBI Accession #

NP_000055.2

UniProt Primary Accession #

P01024

UniProt Secondary Accession #

A7E236

UniProt Related Accession #

P01024

GenBank Nucleotide #

NM_000064.3

NCBI Official Full Name

complement C3 preproprotein

NCBI Official Synonym Full Names

complement C3

NCBI Official Symbol

NCBI Official Synonym Symbols

ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p

UniProt Protein Name

Complement C3

UniProt Synonym Protein Names

C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1

Protein Family

Complement C3

UniProt Gene Name

UniProt Synonym Gene Names

CPAMD1; C3bc; ASP

NCBI Protein Information

complement C3

NCBI Summary for C3

Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients. [provided by RefSeq, Nov 2015]

UniProt Comments for C3

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.

Research Articles on C3

1. Pra1 targets C3 by cleaving C3 at a unique site. This inhibited effector function of the activation fragments. The newly formed C3a-like peptide lacked the C-terminal arginine residue needed for C3a-receptor binding and activation. Pra1 also bound to C3a and C3b generated by human convertases and blocked their effector functions, C3a binding to human C3a receptor, C3 antifungal activity, and C3b deposition.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-C3 antibody

Reference Product	PubMed Publications
CR1 antibody	>410 publications with C3 and CR1
CR2 antibody	>286 publications with C3 and CR2
CD46 antibody	>181 publications with C3 and CD46
CFH antibody	>176 publications with C3 and CFH
CFB antibody	>68 publications with C3 and CFB
ITGAM antibody	>67 publications with C3 and ITGAM
CFI antibody	>65 publications with C3 and CFI
CFHR3 antibody	>21 publications with C3 and CFHR3
MASP1 antibody	>21 publications with C3 and MASP1
CFHR5 antibody	>19 publications with C3 and CFHR5

Pathways associated with anti-C3 antibody

Products by Pathway	Pathway Diagram
Activation Of C3 And C5 Pathway antibodies	Activation Of C3 And C5 Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Alternative Complement Activation Pathway antibodies	Alternative Complement Activation Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Complement Activation, Classical Pathway antibodies	Complement Activation, Classical Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram

Diseases associated with anti-C3 antibody

Disease Name	Pubmed Publications
Kidney Diseases Antibodies	>3478 publications with C3 and Kidney Diseases
Neoplasms Antibodies	>2613 publications with C3 and Neoplasms
Nervous System Diseases Antibodies	>2572 publications with C3 and Nervous System Diseases
Cardiovascular Diseases Antibodies	>2243 publications with C3 and Cardiovascular Diseases
Inflammation Antibodies	>1760 publications with C3 and Inflammation
Proteinuria Antibodies	>1197 publications with C3 and Proteinuria
Lung Diseases Antibodies	>971 publications with C3 and Lung Diseases
Liver Diseases Antibodies	>887 publications with C3 and Liver Diseases
Necrosis Antibodies	>873 publications with C3 and Necrosis
Pain Antibodies	>872 publications with C3 and Pain

Organs/Tissues associated with anti-C3 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>11312 publications with C3 and Blood
Kidney Antibodies	>3236 publications with C3 and Kidney
Bone Antibodies	>2623 publications with C3 and Bone
Skin Antibodies	>2178 publications with C3 and Skin
Brain Antibodies	>1862 publications with C3 and Brain
Liver Antibodies	>1674 publications with C3 and Liver
Muscle Antibodies	>1579 publications with C3 and Muscle
Spinal Cord Antibodies	>1409 publications with C3 and Spinal Cord
Nerve Antibodies	>1238 publications with C3 and Nerve
Lung Antibodies	>1019 publications with C3 and Lung