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FoxO3a Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179222

Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC:… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
FoxO3a, Monoclonal Antibody
Full Product Name
Anti-FoxO3a Rabbit Monoclonal Antibody
Product Gene Name
anti-FoxO3a antibody
Product Synonym Gene Name
FOXO3
Product Synonym Names
Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1; FOXO3; FKHRL1; FOXO3A
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
71277 MW
OMIM
AF032886 mRNA
3D Structure
ModBase 3D Structure for O43524
Immunogen
A synthesized peptide derived from human FoxO3a
Subcellular Localization
Cytoplasm, cytosol. Nucleus. Translocates to the nucleus upon oxidative stress and in the absence of survival factors.
Tissue Specificity
Ubiquitous.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-FoxO3a antibody

Cell Biology; Apoptosis; Intracellular; Associated Proteins; Epigenetics and Nuclear Signaling; Nuclear Signaling Pathways; Nuclear Receptors; Co-Activators/Co-Repressors; Transcription; Domain Families; Forkhead Box; FOXO; Cardiovascular/Immune; Hypoxia
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-FoxO3a antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-FoxO3a antibody
WB: 1:1000-1:2000
ICC/IF: 1:50-1:200

Western Blot (WB) of anti-FoxO3a antibody

Western blot analysis of FOXO3A expression in MCF-7 cell lysate (MBS179222).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXO3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FOXO3
Rabbit Anti-FoxO3a Monoclonal Antibody – Cat# MBS179222

Other Notes

Small volumes of anti-FoxO3a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FoxO3a purchase

MBS179222 is an antibody targeting FoxO3a. Also known as Anti-FoxO3a Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for FoxO3a. It may not necessarily be applicable to this product.

NCBI GI #
4503739
NCBI GeneID
2309
NCBI Accession #
NP_001446.1
UniProt Primary Accession #
O43524
UniProt Secondary Accession #
O15171; Q5T2I7; Q9BZ04; B4DVZ6; E1P5E6
UniProt Related Accession #
O43524
GenBank Nucleotide #
NM_001455.3
NCBI Official Full Name
forkhead box protein O3
NCBI Official Synonym Full Names
forkhead box O3
NCBI Official Symbol
FOXO3
NCBI Official Synonym Symbols
FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
UniProt Protein Name
Forkhead box protein O3
UniProt Synonym Protein Names
AF6q21 protein
UniProt Gene Name
FOXO3
NCBI Protein Information
forkhead box protein O3

NCBI Summary for FoxO3a

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
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UniProt Comments for FoxO3a

Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.
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Research Articles on FoxO3a

1. The result suggests that FOXO3 rs12212067 polymorphism does not play an important role in susceptibility to T. cruzi infection and/or chronic Chagas cardiomyopathy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-FoxO3a antibody

 Reference Product  PubMed Publications
 AKT1 antibody  >51 publications with FoxO3a and AKT1
 CDKN1B antibody  >49 publications with FoxO3a and CDKN1B
 SIRT1 antibody  >48 publications with FoxO3a and SIRT1
 CDKN1A antibody  >21 publications with FoxO3a and CDKN1A
 IKBKB antibody  >17 publications with FoxO3a and IKBKB
 CTNNB1 antibody  >16 publications with FoxO3a and CTNNB1
 SGK1 antibody  >7 publications with FoxO3a and SGK1
 SMAD3 antibody  >6 publications with FoxO3a and SMAD3
 TP53 antibody  >6 publications with FoxO3a and TP53
 SMAD2 antibody  >5 publications with FoxO3a and SMAD2

Pathways associated with anti-FoxO3a antibody

 Products by Pathway  Pathway Diagram
 AKT Phosphorylates Targets In The Nucleus Pathway antibodies  AKT Phosphorylates Targets In The Nucleus Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class I PI3K Signaling Events Pathway antibodies  Class I PI3K Signaling Events Pathway Diagram
 Class I PI3K Signaling Events Mediated By Akt Pathway antibodies  Class I PI3K Signaling Events Mediated By Akt Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram

Diseases associated with anti-FoxO3a antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >242 publications with FoxO3a and Neoplasms
 Carcinoma Antibodies  >71 publications with FoxO3a and Carcinoma
 Breast Neoplasms Antibodies  >48 publications with FoxO3a and Breast Neoplasms
 Cardiovascular Diseases Antibodies  >46 publications with FoxO3a and Cardiovascular Diseases
 Necrosis Antibodies  >38 publications with FoxO3a and Necrosis
 Inflammation Antibodies  >38 publications with FoxO3a and Inflammation
 Heart Diseases Antibodies  >27 publications with FoxO3a and Heart Diseases
 Adenocarcinoma Antibodies  >26 publications with FoxO3a and Adenocarcinoma
 Hypertrophy Antibodies  >24 publications with FoxO3a and Hypertrophy
 Cell Transformation, Neoplastic Antibodies  >21 publications with FoxO3a and Cell Transformation, Neoplastic

Organs/Tissues associated with anti-FoxO3a antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >142 publications with FoxO3a and Blood
 Muscle Antibodies  >141 publications with FoxO3a and Muscle
 Brain Antibodies  >56 publications with FoxO3a and Brain
 Vascular Antibodies  >51 publications with FoxO3a and Vascular
 Heart Antibodies  >50 publications with FoxO3a and Heart
 Lung Antibodies  >37 publications with FoxO3a and Lung
 Liver Antibodies  >36 publications with FoxO3a and Liver
 Bone Antibodies  >33 publications with FoxO3a and Bone
 Ovary Antibodies  >30 publications with FoxO3a and Ovary
 Nerve Antibodies  >26 publications with FoxO3a and Nerve
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