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MiTF Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179231

Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'- CACGTG-3') found in the promoters of target genes, such as BCL2 and… More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MiTF, Monoclonal Antibody
Full Product Name
Anti-MiTF Rabbit Monoclonal Antibody
Product Gene Name
anti-MiTF antibody
Product Synonym Names
Microphthalmia-associated transcription factor; Class E basic helix-loop-helix protein 32; bHLHe32; MITF; BHLHE32
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
58795 MW
OMIM
AB006909 mRNA
3D Structure
ModBase 3D Structure for O75030
Immunogen
A synthesized peptide derived from human MiTF
Subcellular Localization
Nucleus.
Tissue Specificity
Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells. Isoform Mdel is widely expressed in melanocytes, melanoma cell lines and tissues, but almost undetectable in non-melanoma cell lines.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-MiTF antibody

Epigenetics and Nuclear Signaling; Transcription; Domain Families; HLH/Leucine Zipper; Epigenetics and Nuclear Signaling; HLH; Immunology; Innate Immunity; masT Cells; ChIP'ing Antibodies
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-MiTF antibody
Western Blot (WB)
Application Notes for anti-MiTF antibody
WB: 1:500-1:2000

Western Blot (WB) of anti-MiTF antibody

Western blot analysis of MiTF expression in A375 cell lysate (MBS179231).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MITF monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MITF
Rabbit Anti-MiTF Monoclonal Antibody – Cat# MBS179231

Other Notes

Small volumes of anti-MiTF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MiTF purchase

MBS179231 is an antibody targeting MiTF. Also known as Anti-MiTF Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MiTF. It may not necessarily be applicable to this product.

NCBI GI #
4557755
NCBI GeneID
4286
NCBI Accession #
NP_000239.1
UniProt Primary Accession #
O75030
UniProt Secondary Accession #
Q14841; Q9P2V0; Q9P2V1; Q9P2V2; Q9P2Y8; B4DJL2; D3K197; E9PFN0
UniProt Related Accession #
O75030
GenBank Nucleotide #
NM_000248.3
NCBI Official Full Name
microphthalmia-associated transcription factor isoform 4
NCBI Official Synonym Full Names
melanogenesis associated transcription factor
NCBI Official Symbol
MITF
NCBI Official Synonym Symbols
MI; WS2; CMM8; WS2A; COMMAD; bHLHe32
UniProt Protein Name
Microphthalmia-associated transcription factor
UniProt Synonym Protein Names
Class E basic helix-loop-helix protein 32; bHLHe32
Protein Family
Microphthalmia-associated transcription factor
UniProt Gene Name
MITF
UniProt Synonym Gene Names
BHLHE32; bHLHe32
NCBI Protein Information
microphthalmia-associated transcription factor

NCBI Summary for MiTF

The protein encoded by this gene is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. The encoded protein regulates melanocyte development and is responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. [provided by RefSeq, Aug 2017]
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UniProt Comments for MiTF

Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
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Research Articles on MiTF

1. Mutations in MITF gene is associated with Waardenburg syndrome type 2A .

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MiTF antibody

 Reference Product  PubMed Publications
 PAX3 antibody  >85 publications with MiTF and PAX3
 TFE3 antibody  >64 publications with MiTF and TFE3
 CTNNB1 antibody  >59 publications with MiTF and CTNNB1
 TFEB antibody  >37 publications with MiTF and TFEB
 PAX6 antibody  >31 publications with MiTF and PAX6
 FOS antibody  >21 publications with MiTF and FOS
 OTX2 antibody  >16 publications with MiTF and OTX2
 LEF1 antibody  >13 publications with MiTF and LEF1
 PIAS3 antibody  >12 publications with MiTF and PIAS3
 CDK2 antibody  >11 publications with MiTF and CDK2

Pathways associated with anti-MiTF antibody

 Products by Pathway  Pathway Diagram
 IL6-mediated Signaling Events Pathway antibodies  IL6-mediated Signaling Events Pathway Diagram
 Kit Receptor Signaling Pathway antibodies  Kit Receptor Signaling Pathway Diagram
 Melanogenesis Pathway antibodies  Melanogenesis Pathway Diagram
 Melanogenesis Pathway antibodies  Melanogenesis Pathway Diagram
 Melanoma Pathway antibodies  Melanoma Pathway Diagram
 Melanoma Pathway antibodies  Melanoma Pathway Diagram
 Neural Crest Differentiation Pathway antibodies  Neural Crest Differentiation Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram

Diseases associated with anti-MiTF antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >490 publications with MiTF and Neoplasms
 Skin Neoplasms Antibodies  >145 publications with MiTF and Skin Neoplasms
 Neoplasms, Experimental Antibodies  >107 publications with MiTF and Neoplasms, Experimental
 Pigmentation Disorders Antibodies  >96 publications with MiTF and Pigmentation Disorders
 Deafness Antibodies  >57 publications with MiTF and Deafness
 Kidney Diseases Antibodies  >35 publications with MiTF and Kidney Diseases
 Waardenburg syndrome type 2 Antibodies  >30 publications with MiTF and Waardenburg syndrome type 2
 Inflammation Antibodies  >27 publications with MiTF and Inflammation
 Necrosis Antibodies  >23 publications with MiTF and Necrosis
 Drug Toxicity Antibodies  >23 publications with MiTF and Drug Toxicity

Organs/Tissues associated with anti-MiTF antibody

 Organ/Tissue Name  Pubmed Publications
 Uterus Antibodies  >9 publications with MiTF and Uterus
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