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VWF Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179232

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for… More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
VWF/Von Willebrand Factor, Monoclonal Antibody
Full Product Name
Anti-VWF/Von Willebrand Factor Rabbit Monoclonal Antibody
Product Gene Name
anti-VWF antibody
Product Synonym Names
von Willebrand factor; vWF; von Willebrand antigen 2; von Willebrand antigen II; VWF; F8VWF
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
309265 MW
OMIM
X04385 mRNA
3D Structure
ModBase 3D Structure for P04275
Immunogen
A synthesized peptide derived from human VWF
Subcellular Localization
Secreted. Secreted, extracellular space, extracellular matrix. Localized to storage granules.
Tissue Specificity
Plasma.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-VWF antibody

Cardiovascular; Blood; Serum Proteins; Cardiovascular; Platelets; Coagulation; Intrinsic; Extrinsic; Regulatory; Cancer; Invasion/Microenvironment; Angiogenesis; Angiogenic Growth Factors; Stem Cells; Endothelial Progenitors; Endothelial Markers; Kits/Lys
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-VWF antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-VWF antibody
WB: 1:500-1:2000
IHC: 1:50-1:200

Western Blot (WB) of anti-VWF antibody

Western blot analysis of VWF expression in human serum lysate (MBS179232).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VWF monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for VWF
Rabbit Anti-VWF Monoclonal Antibody – Cat# MBS179232

Other Notes

Small volumes of anti-VWF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VWF purchase

MBS179232 is an antibody targeting VWF. Also known as Anti-VWF/Von Willebrand Factor Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for VWF. It may not necessarily be applicable to this product.

NCBI GI #
89191868
NCBI GeneID
7450
NCBI Accession #
NP_000543.2
UniProt Primary Accession #
P04275
UniProt Secondary Accession #
Q8TCE8; Q99806
UniProt Related Accession #
P04275
GenBank Nucleotide #
NM_000552.4
NCBI Official Full Name
von Willebrand factor preproprotein
NCBI Official Synonym Full Names
von Willebrand factor
NCBI Official Symbol
VWF
NCBI Official Synonym Symbols
VWD; F8VWF
UniProt Protein Name
von Willebrand factor
UniProt Synonym Protein Names
von Willebrand antigen 2Alternative name(s):von Willebrand antigen II
Protein Family
von Willebrand factor
UniProt Gene Name
VWF
UniProt Synonym Gene Names
F8VWF; vWF
NCBI Protein Information
von Willebrand factor

NCBI Summary for VWF

This gene encodes a glycoprotein involved in hemostasis. The encoded preproprotein is proteolytically processed following assembly into large multimeric complexes. These complexes function in the adhesion of platelets to sites of vascular injury and the transport of various proteins in the blood. Mutations in this gene result in von Willebrand disease, an inherited bleeding disorder. An unprocessed pseudogene has been found on chromosome 22. [provided by RefSeq, Oct 2015]
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Research Articles on VWF

1. VWF binding to MMRN1 was enhanced by shear exposure and ristocetin, and required VWF A1A2A3 region, specifically the A1 and A3 domains.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-VWF antibody

 Reference Product  PubMed Publications
 ADAMTS13 antibody  >541 publications with VWF and ADAMTS13
 VEGFA antibody  >41 publications with VWF and VEGFA
 GP1BA antibody  >13 publications with VWF and GP1BA
 ITGA2B antibody  >6 publications with VWF and ITGA2B
 GATA2 antibody  >1 publications with VWF and GATA2

Pathways associated with anti-VWF antibody

 Products by Pathway  Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram

Diseases associated with anti-VWF antibody

 Disease Name  Pubmed Publications
 Vascular Diseases Antibodies  >1851 publications with VWF and Vascular Diseases
 Cardiovascular Diseases Antibodies  >1826 publications with VWF and Cardiovascular Diseases
 Thrombosis Antibodies  >1307 publications with VWF and Thrombosis
 Heart Diseases Antibodies  >576 publications with VWF and Heart Diseases
 Inflammation Antibodies  >517 publications with VWF and Inflammation
 Neoplasms Antibodies  >423 publications with VWF and Neoplasms
 Kidney Diseases Antibodies  >303 publications with VWF and Kidney Diseases
 Hypertension Antibodies  >268 publications with VWF and Hypertension
 Necrosis Antibodies  >235 publications with VWF and Necrosis
 Anemia Antibodies  >223 publications with VWF and Anemia

Organs/Tissues associated with anti-VWF antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >5482 publications with VWF and Blood
 Vascular Antibodies  >2737 publications with VWF and Vascular
 Heart Antibodies  >572 publications with VWF and Heart
 Muscle Antibodies  >329 publications with VWF and Muscle
 Bone Antibodies  >293 publications with VWF and Bone
 Kidney Antibodies  >277 publications with VWF and Kidney
 Umbilical Cord Antibodies  >245 publications with VWF and Umbilical Cord
 Liver Antibodies  >242 publications with VWF and Liver
 Brain Antibodies  >231 publications with VWF and Brain
 Skin Antibodies  >223 publications with VWF and Skin
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