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G6PD Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179240

Rabbit Monoclonal G6PD/ Glucose 6 Phosphate Dehydrogenase Antibody. Validated in IF, WB, and tested in Human. More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
G6PD/Glucose 6 Phosphate Dehydrogenase, Monoclonal Antibody
Full Product Name
Anti-G6PD/Glucose 6 Phosphate Dehydrogenase Rabbit Monoclonal Antibody
Product Gene Name
anti-G6PD antibody
Product Synonym Names
Glucose-6-phosphate 1-dehydrogenase; G6PD; 1.1.1.49; G6PD
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
OMIM
X03674 mRNA
3D Structure
ModBase 3D Structure for P11413
Conjugate
No
Immunogen
A synthesized peptide derived from human G6PD

Product Categories/Family for anti-G6PD antibody

Signal Transduction; Metabolism; Energy Metabolism; Cardiovascular; Atherosclerosis; Diabetes Associated; Heart; Cardiac Metabolism; Cancer; Cancer Metabolism; Metabolic Signaling Pathway; Metabolism of Carbohydrates; Metabolism; Pathways and Processes; M
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-G6PD antibody
Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-G6PD antibody
Suggested Dilutions:
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
FC/FACS: 1:100

Western Blot (WB) of anti-G6PD antibody

Western blot analysis of G6PD expression in MCF7 cell lysate (MBS179240).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-G6PD monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for G6PD
Rabbit Anti-G6PD Monoclonal Antibody – Cat# MBS179240

Other Notes

Small volumes of anti-G6PD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for G6PD purchase

MBS179240 is an antibody targeting G6PD. Also known as Anti-G6PD/Glucose 6 Phosphate Dehydrogenase Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for G6PD. It may not necessarily be applicable to this product.

NCBI GI #
109389365
NCBI GeneID
2539
NCBI Accession #
NP_000393.4
UniProt Primary Accession #
P11413
UniProt Secondary Accession #
Q16000; Q16765; Q8IU70; Q8IU88; Q8IUA6; Q96PQ2; D3DWX9
UniProt Related Accession #
P11413
GenBank Nucleotide #
NM_000402.4
NCBI Official Full Name
glucose-6-phosphate 1-dehydrogenase isoform a
NCBI Official Synonym Full Names
glucose-6-phosphate dehydrogenase
NCBI Official Symbol
G6PD
NCBI Official Synonym Symbols
G6PD1
UniProt Protein Name
Glucose-6-phosphate 1-dehydrogenase
UniProt Gene Name
G6PD
UniProt Synonym Gene Names
G6PD
NCBI Protein Information
glucose-6-phosphate 1-dehydrogenase

NCBI Summary for G6PD

This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for G6PD

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.

Research Articles on G6PD

1. We concluded that the MeltPro G6PD assay is useful as a diagnostic or screening tool for G6PD deficiency in clinical settings

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-G6PD antibody

 Reference Product  PubMed Publications
 GPI antibody  >59 publications with G6PD and GPI
 PGD antibody  >57 publications with G6PD and PGD
 TKT antibody  >8 publications with G6PD and TKT
 H6PD antibody  >4 publications with G6PD and H6PD
 INS antibody  >3 publications with G6PD and INS

Pathways associated with anti-G6PD antibody

 Products by Pathway  Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Glycogen Storage Diseases Pathway antibodies  Glycogen Storage Diseases Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram

Diseases associated with anti-G6PD antibody

 Disease Name  Pubmed Publications
 Glucosephosphate Dehydrogenase Deficiency Antibodies  >5005 publications with G6PD and Glucosephosphate Dehydrogenase Deficiency
 Anemia Antibodies  >4761 publications with G6PD and Anemia
 Anemia, Hemolytic Antibodies  >4658 publications with G6PD and Anemia, Hemolytic
 Hemolysis Antibodies  >959 publications with G6PD and Hemolysis
 Poisoning Antibodies  >662 publications with G6PD and Poisoning
 Favism Antibodies  >594 publications with G6PD and Favism
 Nervous System Diseases Antibodies  >372 publications with G6PD and Nervous System Diseases
 Liver Diseases Antibodies  >242 publications with G6PD and Liver Diseases
 Anemia, Hemolytic, Congenital Nonspherocytic Antibodies  >214 publications with G6PD and Anemia, Hemolytic, Congenital Nonspherocytic
 Brain Diseases Antibodies  >197 publications with G6PD and Brain Diseases

Organs/Tissues associated with anti-G6PD antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >3606 publications with G6PD and Blood
 Liver Antibodies  >547 publications with G6PD and Liver
 Kidney Antibodies  >203 publications with G6PD and Kidney
 Muscle Antibodies  >144 publications with G6PD and Muscle
 Bone Antibodies  >142 publications with G6PD and Bone
 Brain Antibodies  >129 publications with G6PD and Brain
 Skin Antibodies  >115 publications with G6PD and Skin
 Bone Marrow Antibodies  >100 publications with G6PD and Bone Marrow
 Lung Antibodies  >85 publications with G6PD and Lung
 Vascular Antibodies  >74 publications with G6PD and Vascular
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