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VASP Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179252

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
VASP, Monoclonal Antibody
Full Product Name
Anti-VASP Rabbit Monoclonal Antibody
Product Gene Name
anti-VASP antibody
Product Synonym Names
Vasodilator-stimulated phosphoprotein; VASP; VASP
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
39830 MW
OMIM
Z46389 mRNA
3D Structure
ModBase 3D Structure for P50552
Immunogen
A synthesized peptide derived from human VASP
Subcellular Localization
Cytoplasm. Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cell junction, tight junction. Cell projection, lamellipodium membrane. Cell projection, filopodium membrane. Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions. Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions. In pre-apoptotic cells, colocalizes with MEFV in large specks (pyroptosomes).
Tissue Specificity
Highly expressed in platelets.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-VASP antibody

Signal Transduction; Cytoskeleton/ECM; Extracellular Matrix; Structures; focal adhesions
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-VASP antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-VASP antibody
WB: 1:500-1:2000
IP: 1:50

Western Blot (WB) of anti-VASP antibody

Western blot analysis of VASP expression in HepG2 cell lysate (MBS179252).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VASP monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for VASP
Rabbit Anti-VASP Monoclonal Antibody – Cat# MBS179252

Other Notes

Small volumes of anti-VASP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VASP purchase

MBS179252 is an antibody targeting VASP. Also known as Anti-VASP Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for VASP. It may not necessarily be applicable to this product.

NCBI GI #
4507869
NCBI GeneID
7408
NCBI Accession #
NP_003361.1
UniProt Primary Accession #
P50552
UniProt Secondary Accession #
Q6PIZ1; Q93035; B2RBT9
UniProt Related Accession #
P50552
GenBank Nucleotide #
NP_003361.1
NCBI Official Full Name
vasodilator-stimulated phosphoprotein
NCBI Official Synonym Full Names
vasodilator stimulated phosphoprotein
NCBI Official Symbol
VASP
UniProt Protein Name
Vasodilator-stimulated phosphoprotein
Protein Family
Protein
UniProt Gene Name
VASP
UniProt Synonym Gene Names
VASP
NCBI Protein Information
vasodilator-stimulated phosphoprotein

NCBI Summary for VASP

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq, Jul 2008]
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UniProt Comments for VASP

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
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Research Articles on VASP

1. Our results reveal a dual role of VASP in endothelial permeability. In addition to its well-documented function in barrier integrity, we show that S-nitrosylation of VASP contributes to the onset of endothelial permeability.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-VASP antibody

 Reference Product  PubMed Publications
 ZYX antibody  >33 publications with VASP and ZYX
 PFN1 antibody  >24 publications with VASP and PFN1
 LPP antibody  >7 publications with VASP and LPP
 FYB antibody  >7 publications with VASP and FYB
 PRKG1 antibody  >6 publications with VASP and PRKG1
 PFN2 antibody  >5 publications with VASP and PFN2
 TES antibody  >5 publications with VASP and TES
 ABI1 antibody  >3 publications with VASP and ABI1
 APBB1IP antibody  >3 publications with VASP and APBB1IP

Pathways associated with anti-VASP antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Cell Junction Organization Pathway antibodies  Cell Junction Organization Pathway Diagram
 Cell-Cell Communication Pathway antibodies  Cell-Cell Communication Pathway Diagram
 Cell-extracellular Matrix Interactions Pathway antibodies  Cell-extracellular Matrix Interactions Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 E-cadherin Signaling In Keratinocytes Pathway antibodies  E-cadherin Signaling In Keratinocytes Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram

Diseases associated with anti-VASP antibody

 Disease Name  Pubmed Publications
 Thrombosis Antibodies  >61 publications with VASP and Thrombosis
 Neoplasms Antibodies  >53 publications with VASP and Neoplasms
 Pain Antibodies  >51 publications with VASP and Pain
 Disease Models, Animal Antibodies  >31 publications with VASP and Disease Models, Animal
 Nervous System Diseases Antibodies  >26 publications with VASP and Nervous System Diseases
 Inflammation Antibodies  >24 publications with VASP and Inflammation
 Heart Failure Antibodies  >17 publications with VASP and Heart Failure
 Breast Neoplasms Antibodies  >15 publications with VASP and Breast Neoplasms
 Atherosclerosis Antibodies  >13 publications with VASP and Atherosclerosis
 Hypertension Antibodies  >13 publications with VASP and Hypertension

Organs/Tissues associated with anti-VASP antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >320 publications with VASP and Blood
 Vascular Antibodies  >139 publications with VASP and Vascular
 Muscle Antibodies  >80 publications with VASP and Muscle
 Brain Antibodies  >62 publications with VASP and Brain
 Embryonic Tissue Antibodies  >36 publications with VASP and Embryonic Tissue
 Kidney Antibodies  >30 publications with VASP and Kidney
 Lung Antibodies  >27 publications with VASP and Lung
 Liver Antibodies  >15 publications with VASP and Liver
 Bone Antibodies  >13 publications with VASP and Bone
 Eye Antibodies  >8 publications with VASP and Eye
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