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Tyrosinase (TYR) Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179266

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6… More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Tyrosinase, Monoclonal Antibody
Full Product Name
Anti-Tyrosinase Rabbit Monoclonal Antibody
Product Gene Name
anti-TYR antibody
Product Synonym Names
Tyrosinase; 1.14.18.1; LB24-AB; Monophenol monooxygenase; SK29-AB; Tumor rejection antigen AB; TYR
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
60393 MW
OMIM
M27160 mRNA
3D Structure
ModBase 3D Structure for P14679
Immunogen
A synthesized peptide derived from human Tyrosinase
Subcellular Localization
Melanosome membrane; Single-pass type I membrane protein.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-TYR antibody

Neuroscience; Neurotransmission; Intracellular Signaling; Phosphatases; Signal Transduction; Metabolism; Amino Acids; Protein Phosphorylation; tyrosine Phosphatases; Metabolism; Pathways and Processes; Metabolic Signaling Pathways; Amino Acid Metabolism
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-TYR antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-TYR antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200

Western Blot (WB) of anti-TYR antibody

Western blot analysis of Tyrosinase expression in human melanoma lysate (MBS179266).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TYR monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TYR
Rabbit Anti-TYR Monoclonal Antibody – Cat# MBS179266

Other Notes

Small volumes of anti-TYR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TYR purchase

MBS179266 is an antibody targeting TYR. Also known as Anti-Tyrosinase Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TYR. It may not necessarily be applicable to this product.

NCBI GI #
4507753
NCBI GeneID
7299
NCBI Accession #
NP_000363.1
UniProt Primary Accession #
P14679
UniProt Secondary Accession #
Q15675; Q15676; Q15680; Q8TAK4; Q9BYY0; Q9BZX1
UniProt Related Accession #
P14679
GenBank Nucleotide #
NM_000372.4
NCBI Official Full Name
tyrosinase
NCBI Official Synonym Full Names
tyrosinase
NCBI Official Symbol
TYR
NCBI Official Synonym Symbols
ATN; CMM8; OCA1; OCA1A; OCAIA; SHEP3
UniProt Protein Name
Tyrosinase
UniProt Synonym Protein Names
LB24-AB; Monophenol monooxygenase; SK29-AB; Tumor rejection antigen AB
Protein Family
Tyrosinase
UniProt Gene Name
TYR
NCBI Protein Information
tyrosinase

NCBI Summary for TYR

The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene. [provided by RefSeq, Oct 2008]
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UniProt Comments for TYR

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone.
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Research Articles on TYR

1. Mutations in tyrosinase gene is associated with Oculocutaneous albinism type 1.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TYR antibody

 Reference Product  PubMed Publications
 TYRP1 antibody  >98 publications with TYR and TYRP1

Pathways associated with anti-TYR antibody

 Products by Pathway  Pathway Diagram
 (S)-reticuline Biosynthesis II Pathway antibodies  (S)-reticuline Biosynthesis II Pathway Diagram
 (S)-reticuline Biosynthesis II Pathway antibodies  (S)-reticuline Biosynthesis II Pathway Diagram
 Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway antibodies  Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway Diagram
 Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway antibodies  Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway Diagram
 L-dopachrome Biosynthesis Pathway antibodies  L-dopachrome Biosynthesis Pathway Diagram
 L-dopachrome Biosynthesis Pathway antibodies  L-dopachrome Biosynthesis Pathway Diagram
 Melanogenesis Pathway antibodies  Melanogenesis Pathway Diagram
 Melanogenesis Pathway antibodies  Melanogenesis Pathway Diagram
 Riboflavin Metabolism Pathway antibodies  Riboflavin Metabolism Pathway Diagram
 Riboflavin Metabolism Pathway antibodies  Riboflavin Metabolism Pathway Diagram

Diseases associated with anti-TYR antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1870 publications with TYR and Neoplasms
 Nervous System Diseases Antibodies  >740 publications with TYR and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >389 publications with TYR and Cardiovascular Diseases
 Pain Antibodies  >378 publications with TYR and Pain
 Inflammation Antibodies  >331 publications with TYR and Inflammation
 Melanoma Antibodies  >312 publications with TYR and Melanoma
 Neoplasms, Experimental Antibodies  >264 publications with TYR and Neoplasms, Experimental
 Liver Diseases Antibodies  >200 publications with TYR and Liver Diseases
 Hypertension Antibodies  >190 publications with TYR and Hypertension
 Necrosis Antibodies  >185 publications with TYR and Necrosis

Organs/Tissues associated with anti-TYR antibody

 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >460 publications with TYR and Skin
 Eye Antibodies  >255 publications with TYR and Eye
 Thyroid Antibodies  >170 publications with TYR and Thyroid
 Connective Tissue Antibodies  >128 publications with TYR and Connective Tissue
 Mammary Gland Antibodies  >40 publications with TYR and Mammary Gland
 Ear Antibodies  >28 publications with TYR and Ear
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