Rabbit Anti-SLC6A3 Monoclonal Antibody – Cat# MBS179290

Western blot analysis of Dopamine Transporter expression in HeLa cell lysate (MBS179290).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC6A3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SLC6A3

Dopamine Transporter (SLC6A3) Antibody – Rabbit Monoclonal

Name: SLC6A3 Antibody; Monoclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179290
Price: 485 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179290

Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Dopamine Transporter (SLC6A3), Monoclonal Antibody

Full Product Name

Anti-Dopamine Transporter Rabbit Monoclonal Antibody

Product Gene Name

anti-SLC6A3 antibody

Product Synonym Names

Sodium-dependent dopamine transporter; DA transporter; DAT; Solute carrier family 6 member 3; SLC6A3; DAT1

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

68495 MW

OMIM

M95167 mRNA

3D Structure

ModBase 3D Structure for Q01959

Immunogen

A synthesized peptide derived from human Dopamine Transporter

Subcellular Localization

Cell membrane; Multi- pass membrane protein.

Tissue Specificity

Highly expressed in substantia nigra.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-SLC6A3 antibody

Neuroscience; neurotransmitter; biogenic amines; dopamine; Neuroscience; transporters

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-SLC6A3 antibody

Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)

Application Notes for anti-SLC6A3 antibody

WB: 1:1000-1:5000
IP: 1:50
FC/FACS: 1:50

Other Notes

Small volumes of anti-SLC6A3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SLC6A3 purchase

MBS179290 is an antibody targeting SLC6A3. Also known as Anti-Dopamine Transporter Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SLC6A3. It may not necessarily be applicable to this product.

NCBI GI #

4507041

NCBI GeneID

6531

NCBI Accession #

NP_001035.1

UniProt Primary Accession #

Q01959

UniProt Secondary Accession #

Q14996; A2RUN4

UniProt Related Accession #

Q01959

GenBank Nucleotide #

NM_001044.4

NCBI Official Full Name

sodium-dependent dopamine transporter

NCBI Official Synonym Full Names

solute carrier family 6 member 3

NCBI Official Symbol

SLC6A3

NCBI Official Synonym Symbols

DAT; DAT1; PKDYS

UniProt Protein Name

Sodium-dependent dopamine transporter

UniProt Synonym Protein Names

Solute carrier family 6 member 3

Protein Family

Sodium-dependent dopamine transporter

UniProt Gene Name

SLC6A3

UniProt Synonym Gene Names

DAT1; DA transporter; DAT

NCBI Protein Information

sodium-dependent dopamine transporter

NCBI Summary for SLC6A3

This gene encodes a dopamine transporter which is a member of the sodium- and chloride-dependent neurotransmitter transporter family. The 3' UTR of this gene contains a 40 bp tandem repeat, referred to as a variable number tandem repeat or VNTR, which can be present in 3 to 11 copies. Variation in the number of repeats is associated with idiopathic epilepsy, attention-deficit hyperactivity disorder, dependence on alcohol and cocaine, susceptibility to Parkinson disease and protection against nicotine dependence.[provided by RefSeq, Nov 2009]

Research Articles on SLC6A3

1. Studied neural activation during an emotional response inhibition task across different DAT1 genotypes in healthy individuals, showed that genotypic variation influencing basal dopamine levels can alter the neural basis of emotional processing and response inhibition. This may indicate that 9R carriers exert more effort to overcome increased basal dopamine activation when inhibiting responses in emotional contexts.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SLC6A3 antibody

Reference Product	PubMed Publications
DRD2 antibody	>94 publications with SLC6A3 and DRD2
SNCA antibody	>12 publications with SLC6A3 and SNCA
HPRD antibody	>1 publications with SLC6A3 and HPRD
PICK1 antibody	>1 publications with SLC6A3 and PICK1
STX1A antibody	>1 publications with SLC6A3 and STX1A

Pathways associated with anti-SLC6A3 antibody

Products by Pathway	Pathway Diagram
Alcoholism Pathway antibodies	Alcoholism Pathway Diagram
Alcoholism Pathway antibodies	Alcoholism Pathway Diagram
Alpha-synuclein Signaling Pathway antibodies	Alpha-synuclein Signaling Pathway Diagram
Amphetamine Addiction Pathway antibodies	Amphetamine Addiction Pathway Diagram
Amphetamine Addiction Pathway antibodies	Amphetamine Addiction Pathway Diagram
Cocaine Addiction Pathway antibodies	Cocaine Addiction Pathway Diagram
Cocaine Addiction Pathway antibodies	Cocaine Addiction Pathway Diagram
Dopamine Clearance From The Synaptic Cleft Pathway antibodies	Dopamine Clearance From The Synaptic Cleft Pathway Diagram
Dopaminergic Synapse Pathway antibodies	Dopaminergic Synapse Pathway Diagram
Dopaminergic Synapse Pathway antibodies	Dopaminergic Synapse Pathway Diagram

Diseases associated with anti-SLC6A3 antibody

Disease Name	Pubmed Publications
Attention Deficit Disorder with Hyperactivity Antibodies	>229 publications with SLC6A3 and Attention Deficit Disorder with Hyperactivity
Nervous System Diseases Antibodies	>197 publications with SLC6A3 and Nervous System Diseases
Brain Diseases Antibodies	>164 publications with SLC6A3 and Brain Diseases
Movement Disorders Antibodies	>137 publications with SLC6A3 and Movement Disorders
Parkinsonian Disorders Antibodies	>116 publications with SLC6A3 and Parkinsonian Disorders
Parkinson Disease Antibodies	>90 publications with SLC6A3 and Parkinson Disease
Schizophrenia Antibodies	>75 publications with SLC6A3 and Schizophrenia
Drug Toxicity Antibodies	>50 publications with SLC6A3 and Drug Toxicity
Cocaine-Related Disorders Antibodies	>49 publications with SLC6A3 and Cocaine-Related Disorders
Nerve Degeneration Antibodies	>41 publications with SLC6A3 and Nerve Degeneration

Organs/Tissues associated with anti-SLC6A3 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>670 publications with SLC6A3 and Brain
Ganglia Antibodies	>55 publications with SLC6A3 and Ganglia
Kidney Antibodies	>39 publications with SLC6A3 and Kidney
Lung Antibodies	>4 publications with SLC6A3 and Lung