Rabbit Anti-GATA1 Monoclonal Antibody – Cat# MBS179313

Western blot analysis of GATA1 expression in K562 cell lysate (MBS179313).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GATA1

GATA1 Antibody – Rabbit Monoclonal

Name: GATA1 Antibody; Monoclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179313
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179313

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

GATA1, Monoclonal Antibody

Full Product Name

Anti-GATA1 Rabbit Monoclonal Antibody

Product Gene Name

anti-GATA1 antibody

Product Synonym Names

Erythroid transcription factor; Eryf1; GATA-binding factor 1; GATA-1; GF-1; NF-E1 DNA-binding protein; GATA1; ERYF1; GF1

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

42751 MW

OMIM

X17254 mRNA

3D Structure

ModBase 3D Structure for P15976

Immunogen

A synthesized peptide derived from human GATA1

Subcellular Localization

Nucleus.

Tissue Specificity

Erythrocytes.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-GATA1 antibody

Epigenetics and Nuclear Signaling; Transcription; Domain Families; zinc finger; Stem Cells; Hematopoietic Progenitors; Intracellular molecules; Developmental Biology; Organogenesis; hematopoietic system development

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-GATA1 antibody

Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Application Notes for anti-GATA1 antibody

WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50

Other Notes

Small volumes of anti-GATA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GATA1 purchase

MBS179313 is an antibody targeting GATA1. Also known as Anti-GATA1 Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GATA1. It may not necessarily be applicable to this product.

NCBI GI #

4503925

NCBI GeneID

2623

NCBI Accession #

NP_002040.1

UniProt Primary Accession #

P15976

UniProt Secondary Accession #

Q96GB8

UniProt Related Accession #

P15976

GenBank Nucleotide #

NM_002049.3

NCBI Official Full Name

erythroid transcription factor

NCBI Official Synonym Full Names

GATA binding protein 1

NCBI Official Symbol

GATA1

NCBI Official Synonym Symbols

GF1; GF-1; NFE1; XLTT; ERYF1; NF-E1; XLANP; XLTDA; GATA-1

UniProt Protein Name

Erythroid transcription factor

UniProt Synonym Protein Names

Eryf1; GATA-binding factor 1; GATA-1; GF-1; NF-E1 DNA-binding protein

Protein Family

Erythroid transcription factor

UniProt Gene Name

GATA1

UniProt Synonym Gene Names

ERYF1; GF1; GATA-1; GF-1

NCBI Protein Information

erythroid transcription factor

NCBI Summary for GATA1

This gene encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. [provided by RefSeq, Jul 2008]

UniProt Comments for GATA1

Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed:24245781).

Research Articles on GATA1

1. expression of GATA1 effectively rescued maturation of Primary myelofibrosis megakaryocytes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GATA1 antibody

Reference Product	PubMed Publications
ZFPM1 antibody	>65 publications with GATA1 and ZFPM1
LMO2 antibody	>52 publications with GATA1 and LMO2
FLI1 antibody	>28 publications with GATA1 and FLI1
STAT3 antibody	>15 publications with GATA1 and STAT3
CASP3 antibody	>8 publications with GATA1 and CASP3
ZFPM2 antibody	>7 publications with GATA1 and ZFPM2
CREBBP antibody	>6 publications with GATA1 and CREBBP
SPI1 antibody	>5 publications with GATA1 and SPI1
MED1 antibody	>4 publications with GATA1 and MED1
CEBPE antibody	>4 publications with GATA1 and CEBPE

Pathways associated with anti-GATA1 antibody

Products by Pathway	Pathway Diagram
C-MYB Transcription Factor Network Pathway antibodies	C-MYB Transcription Factor Network Pathway Diagram
Factors Involved In Megakaryocyte Development And Platelet Production Pathway antibodies	Factors Involved In Megakaryocyte Development And Platelet Production Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
IL-3 Signaling Pathway antibodies	IL-3 Signaling Pathway Diagram
Notch-mediated HES/HEY Network Pathway antibodies	Notch-mediated HES/HEY Network Pathway Diagram
Signaling Events Mediated By HDAC Class I Pathway antibodies	Signaling Events Mediated By HDAC Class I Pathway Diagram
Signaling Events Mediated By HDAC Class II Pathway antibodies	Signaling Events Mediated By HDAC Class II Pathway Diagram

Diseases associated with anti-GATA1 antibody

Disease Name	Pubmed Publications
THROMBOCYTOPENIA, X-LINKED, WITH OR WITHOUT DYSERYTHROPOIETIC ANEMIA Antibodies	>650 publications with GATA1 and THROMBOCYTOPENIA, X-LINKED, WITH OR WITHOUT DYSERYTHROPOIETIC ANEMIA
Leukemia Antibodies	>362 publications with GATA1 and Leukemia
Neoplasms Antibodies	>309 publications with GATA1 and Neoplasms
Leukemia, Myeloid, Acute Antibodies	>227 publications with GATA1 and Leukemia, Myeloid, Acute
Bone Marrow Diseases Antibodies	>195 publications with GATA1 and Bone Marrow Diseases
Down Syndrome Antibodies	>153 publications with GATA1 and Down Syndrome
Anemia Antibodies	>125 publications with GATA1 and Anemia
Thrombocytopenia Antibodies	>63 publications with GATA1 and Thrombocytopenia
Chromosome Aberrations Antibodies	>44 publications with GATA1 and Chromosome Aberrations
Disease Models, Animal Antibodies	>34 publications with GATA1 and Disease Models, Animal

Organs/Tissues associated with anti-GATA1 antibody

Organ/Tissue Name	Pubmed Publications
Bone Marrow Antibodies	>166 publications with GATA1 and Bone Marrow
Lung Antibodies	>21 publications with GATA1 and Lung
Muscle Antibodies	>11 publications with GATA1 and Muscle
Connective Tissue Antibodies	>7 publications with GATA1 and Connective Tissue
Uncharacterized Tissue Antibodies	>1 publications with GATA1 and Uncharacterized Tissue