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KDM1/LSD1 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179354

Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is… More details

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Product Overview

Online Datasheet in PDF
Product Name
KDM1/LSD1, Monoclonal Antibody
Full Product Name
Anti-KDM1/LSD1 Rabbit Monoclonal Antibody
Product Gene Name
anti-KDM1/LSD1 antibody
Product Synonym Gene Name
KDM1A
Product Synonym Names
Lysine-specific histone demethylase 1A; 1.-.-.-; BRAF35-HDAC complex protein BHC110; Flavin-containing amine oxidase domain-containing protein 2; KDM1A; AOF2; KDM1; KIAA0601; LSD1
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
92903 MW
OMIM
AB011173 mRNA
3D Structure
ModBase 3D Structure for O60341
Immunogen
A synthesized peptide derived from human KDM1/LSD1
Subcellular Localization
Nucleus.
Tissue Specificity
Ubiquitously expressed.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-KDM1/LSD1 antibody
Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-KDM1/LSD1 antibody
WB: 1:1000-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:30

Immunohistochemistry (IHC) of anti-KDM1/LSD1 antibody

Immunohistochemical analysis of paraffin-embedded human colon, using KDM1/LSD1 Antibody(MBS179354)
KDM1A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-KDM1A Antibody (MBS179354)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-KDM1/LSD1 Monoclonal Antibody – Cat# MBS179354

Western Blot (WB) of anti-KDM1/LSD1 antibody

Western blot analysis of KDM1/LSD1 expression in Hela cell lysate (MBS179354).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KDM1A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KDM1A
Rabbit Anti-KDM1/LSD1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS179354

Other Notes

Small volumes of anti-KDM1/LSD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KDM1/LSD1 purchase

MBS179354 is an antibody targeting KDM1/LSD1. Also known as Anti-KDM1/LSD1 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for KDM1/LSD1. It may not necessarily be applicable to this product.

NCBI GI #
58761544
NCBI GeneID
23028
NCBI Accession #
NP_001009999.1
UniProt Primary Accession #
O60341
UniProt Secondary Accession #
Q5TH94; Q5TH95; Q86VT7; Q8IXK4; Q8NDP6; Q8TAZ3; Q96AW4; A8MWP9
UniProt Related Accession #
O60341
GenBank Nucleotide #
NP_001009999.1
NCBI Official Full Name
lysine-specific histone demethylase 1A isoform a
NCBI Official Synonym Full Names
lysine demethylase 1A
NCBI Official Symbol
KDM1A
NCBI Official Synonym Symbols
AOF2; CPRF; KDM1; LSD1; BHC110
UniProt Protein Name
Lysine-specific histone demethylase 1A
UniProt Synonym Protein Names
BRAF35-HDAC complex protein BHC110; Flavin-containing amine oxidase domain-containing protein 2
UniProt Gene Name
KDM1A
UniProt Synonym Gene Names
AOF2; KDM1; KIAA0601; LSD1
NCBI Protein Information
lysine-specific histone demethylase 1A

NCBI Summary for KDM1/LSD1

This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
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UniProt Comments for KDM1/LSD1

Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7.
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Research Articles on KDM1/LSD1

1. LSD1 inhibition with HCI-2509 decreases the c-MYC level in poorly differentiated prostate cancer cell lines.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-KDM1/LSD1 antibody

 Products by Pathway  Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Coregulation Of Androgen Receptor Activity Pathway antibodies  Coregulation Of Androgen Receptor Activity Pathway Diagram
 Factors Involved In Megakaryocyte Development And Platelet Production Pathway antibodies  Factors Involved In Megakaryocyte Development And Platelet Production Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Notch Signaling Pathway antibodies  Notch Signaling Pathway Diagram
 Notch-mediated HES/HEY Network Pathway antibodies  Notch-mediated HES/HEY Network Pathway Diagram

Diseases associated with anti-KDM1/LSD1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >54 publications with KDM1/LSD1 and Neoplasms
 Breast Neoplasms Antibodies  >14 publications with KDM1/LSD1 and Breast Neoplasms
 Colorectal Neoplasms Antibodies  >7 publications with KDM1/LSD1 and Colorectal Neoplasms
 Intestinal Neoplasms Antibodies  >5 publications with KDM1/LSD1 and Intestinal Neoplasms
 Lymphoma Antibodies  >5 publications with KDM1/LSD1 and Lymphoma
 Disease Models, Animal Antibodies  >4 publications with KDM1/LSD1 and Disease Models, Animal
 Neoplasm Metastasis Antibodies  >4 publications with KDM1/LSD1 and Neoplasm Metastasis
 Neoplasm Invasiveness Antibodies  >4 publications with KDM1/LSD1 and Neoplasm Invasiveness
 Liver Diseases Antibodies  >3 publications with KDM1/LSD1 and Liver Diseases
 Inflammation Antibodies  >3 publications with KDM1/LSD1 and Inflammation

Organs/Tissues associated with anti-KDM1/LSD1 antibody

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >17 publications with KDM1/LSD1 and Nerve
 Prostate Antibodies  >11 publications with KDM1/LSD1 and Prostate
 Brain Antibodies  >9 publications with KDM1/LSD1 and Brain
 Blood Antibodies  >8 publications with KDM1/LSD1 and Blood
 Lung Antibodies  >6 publications with KDM1/LSD1 and Lung
 Heart Antibodies  >4 publications with KDM1/LSD1 and Heart
 Embryonic Tissue Antibodies  >4 publications with KDM1/LSD1 and Embryonic Tissue
 Muscle Antibodies  >4 publications with KDM1/LSD1 and Muscle
 Bone Antibodies  >4 publications with KDM1/LSD1 and Bone
 Liver Antibodies  >3 publications with KDM1/LSD1 and Liver
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