Rabbit Anti-PGR Monoclonal Antibody – Cat# MBS179358

Western blot analysis of Calreticulin expression in T47 D cell lysate (MBS179358).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGR monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PGR

Progesterone Receptor (PGR) Antibody – Rabbit Monoclonal

Name: PGR Antibody; Monoclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179358
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179358

The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling… More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Progesterone Receptor (PGR), Monoclonal Antibody

Full Product Name

Anti-Progesterone Receptor Rabbit Monoclonal Antibody

Product Gene Name

anti-PGR antibody

Product Synonym Names

Progesterone receptor; PR; Nuclear receptor subfamily 3 group C member 3; PGR; NR3C3

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

98981 MW

OMIM

X51730 mRNA

3D Structure

ModBase 3D Structure for P06401

Immunogen

A synthesized peptide derived from human Progesterone Receptor

Subcellular Localization

Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-PGR antibody

Epigenetics and Nuclear Signaling; Transcription; Domain Families; zinc finger; Signal Transduction; Signaling Pathway; nuclear hormone Receptors; progesterone; Neuroscience; endocrine system; gonadotrophic axis; Epigenetics and Nuclear Signaling; Nuclear

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-PGR antibody

Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Application Notes for anti-PGR antibody

WB: 1:1000-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50

Other Notes

Small volumes of anti-PGR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PGR purchase

MBS179358 is an antibody targeting PGR. Also known as Anti-Progesterone Receptor Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PGR. It may not necessarily be applicable to this product.

NCBI GI #

110611914

NCBI GeneID

5241

NCBI Accession #

NP_000917.3

UniProt Primary Accession #

P06401

UniProt Secondary Accession #

Q8TDS3; Q9UPF7; A7LQ08; A7X8B0; B4E3T0

UniProt Related Accession #

P06401

GenBank Nucleotide #

NM_000926.4

NCBI Official Full Name

progesterone receptor isoform B

NCBI Official Synonym Full Names

progesterone receptor

NCBI Official Symbol

PGR

NCBI Official Synonym Symbols

PR; NR3C3

UniProt Protein Name

Progesterone receptor

UniProt Synonym Protein Names

Nuclear receptor subfamily 3 group C member 3

Protein Family

Progesterone receptor

UniProt Gene Name

PGR

UniProt Synonym Gene Names

NR3C3; PR

NCBI Protein Information

progesterone receptor

NCBI Summary for PGR

This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce several transcript variants, both protein coding and non-protein coding. Two of the isoforms (A and B) are identical except for an additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap. [provided by RefSeq, Sep 2015]

UniProt Comments for PGR

The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Depending on the isoform, progesterone receptor functions as transcriptional activator or repressor.

Research Articles on PGR

1. We investigate the impact of central histology (n = 772), immunohistochemistry for intrinsic subtyping and IHC4, and dichotomous (GG) or continuous (GGI) genomic grade (n = 472) on patient outcome and benefit from taxane chemotherapy, focusing on HR+/HER2(HR: ER and/or PR)- patients (n = 459)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PGR antibody

Reference Product	PubMed Publications
ESR1 antibody	>89 publications with PGR and ESR1
SRC antibody	>6 publications with PGR and SRC
KLF9 antibody	>6 publications with PGR and KLF9
SP1 antibody	>4 publications with PGR and SP1
NCOA3 antibody	>3 publications with PGR and NCOA3
RELA antibody	>2 publications with PGR and RELA
NCOA1 antibody	>2 publications with PGR and NCOA1

Pathways associated with anti-PGR antibody

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Oocyte Meiosis Pathway antibodies	Oocyte Meiosis Pathway Diagram
Oocyte Meiosis Pathway antibodies	Oocyte Meiosis Pathway Diagram
Ovarian Infertility Genes Pathway antibodies	Ovarian Infertility Genes Pathway Diagram
Progesterone-mediated Oocyte Maturation Pathway antibodies	Progesterone-mediated Oocyte Maturation Pathway Diagram

Diseases associated with anti-PGR antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>1596 publications with PGR and Neoplasms
Breast Neoplasms Antibodies	>1319 publications with PGR and Breast Neoplasms
Carcinoma Antibodies	>611 publications with PGR and Carcinoma
Adenocarcinoma Antibodies	>312 publications with PGR and Adenocarcinoma
Recurrence Antibodies	>292 publications with PGR and Recurrence
Neoplasms, Experimental Antibodies	>123 publications with PGR and Neoplasms, Experimental
Uterine Neoplasms Antibodies	>89 publications with PGR and Uterine Neoplasms
Endometrial Neoplasms Antibodies	>71 publications with PGR and Endometrial Neoplasms
Mammary Neoplasms, Animal Antibodies	>68 publications with PGR and Mammary Neoplasms, Animal
Ovarian Neoplasms Antibodies	>67 publications with PGR and Ovarian Neoplasms