Rabbit Anti-WNT5A Monoclonal Antibody – Cat# MBS179365

Western blot analysis of Wnt5a expression in HeLa cell lysate (MBS179365).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT5A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for WNT5A

WNT5A Antibody – Rabbit Monoclonal

Name: WNT5A Antibody; Monoclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179365
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179365

Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the… More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Wnt5a, Monoclonal Antibody

Full Product Name

Anti-Wnt5a Rabbit Monoclonal Antibody

Product Gene Name

anti-Wnt5a antibody

Product Synonym Names

Protein Wnt-5a; WNT5A

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

42339 MW

OMIM

L20861 mRNA

3D Structure

ModBase 3D Structure for P41221

Immunogen

A synthesized peptide derived from human Wnt5a

Subcellular Localization

Secreted, extracellular space, extracellular matrix.

Tissue Specificity

Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells (at protein level).

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-Wnt5a antibody

Neuroscience; Neurology Process; Neural Signal Transduction; Stem Cells; Signaling Pathways; wnt; Secreted; Cancer; Oncoproteins/Suppressors; Tumor Suppressors; ptc & wnt Pathway

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-Wnt5a antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)

Application Notes for anti-Wnt5a antibody

WB: 1:500-1:1000
ICC/IF: 1:50-1:200
FC/FACS: 1:20

Other Notes

Small volumes of anti-Wnt5a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Wnt5a purchase

MBS179365 is an antibody targeting Wnt5a. Also known as Anti-Wnt5a Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Wnt5a. It may not necessarily be applicable to this product.

NCBI GI #

371502087

NCBI GeneID

7474

NCBI Accession #

NP_001243034.1

UniProt Primary Accession #

P41221

UniProt Secondary Accession #

Q6P278; A8K4A4

UniProt Related Accession #

P41221

GenBank Nucleotide #

NM_001256105.1

NCBI Official Full Name

protein Wnt-5a isoform 2

NCBI Official Synonym Full Names

Wnt family member 5A

NCBI Official Symbol

WNT5A

NCBI Official Synonym Symbols

hWNT5A

UniProt Protein Name

Protein Wnt-5a

Protein Family

Protein

UniProt Gene Name

WNT5A

NCBI Protein Information

protein Wnt-5a

NCBI Summary for Wnt5a

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Research Articles on Wnt5a

1. WNT5A and IL-6 are connected through a positive feedback loop in melanoma cells

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Wnt5a antibody

Reference Product	PubMed Publications
FZD2 antibody	>37 publications with Wnt5a and FZD2
LRP5 antibody	>31 publications with Wnt5a and LRP5
FZD5 antibody	>24 publications with Wnt5a and FZD5
FZD1 antibody	>21 publications with Wnt5a and FZD1
DVL2 antibody	>20 publications with Wnt5a and DVL2
FZD7 antibody	>16 publications with Wnt5a and FZD7
FZD4 antibody	>12 publications with Wnt5a and FZD4
DVL3 antibody	>10 publications with Wnt5a and DVL3
FZD8 antibody	>5 publications with Wnt5a and FZD8
DVL1 antibody	>5 publications with Wnt5a and DVL1

Pathways associated with anti-Wnt5a antibody

Products by Pathway	Pathway Diagram
Basal Cell Carcinoma Pathway antibodies	Basal Cell Carcinoma Pathway Diagram
Basal Cell Carcinoma Pathway antibodies	Basal Cell Carcinoma Pathway Diagram
Class B/2 (Secretin Family Receptors) Pathway antibodies	Class B/2 (Secretin Family Receptors) Pathway Diagram
DNA Damage Response (only ATM Dependent) Pathway antibodies	DNA Damage Response (only ATM Dependent) Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
HTLV-I Infection Pathway antibodies	HTLV-I Infection Pathway Diagram
HTLV-I Infection Pathway antibodies	HTLV-I Infection Pathway Diagram
Hedgehog Signaling Pathway antibodies	Hedgehog Signaling Pathway Diagram
Hedgehog Signaling Pathway antibodies	Hedgehog Signaling Pathway Diagram
Hippo Signaling Pathway antibodies	Hippo Signaling Pathway Diagram

Diseases associated with anti-Wnt5a antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>243 publications with Wnt5a and Neoplasms
Breast Neoplasms Antibodies	>44 publications with Wnt5a and Breast Neoplasms
Neoplasm Metastasis Antibodies	>43 publications with Wnt5a and Neoplasm Metastasis
Inflammation Antibodies	>37 publications with Wnt5a and Inflammation
Adenocarcinoma Antibodies	>35 publications with Wnt5a and Adenocarcinoma
Neoplasms, Experimental Antibodies	>33 publications with Wnt5a and Neoplasms, Experimental
Female Urogenital Diseases Antibodies	>28 publications with Wnt5a and Female Urogenital Diseases
Skin Neoplasms Antibodies	>26 publications with Wnt5a and Skin Neoplasms
Nervous System Diseases Antibodies	>25 publications with Wnt5a and Nervous System Diseases
Cell Transformation, Neoplastic Antibodies	>20 publications with Wnt5a and Cell Transformation, Neoplastic

Organs/Tissues associated with anti-Wnt5a antibody

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>136 publications with Wnt5a and Bone
Embryonic Tissue Antibodies	>127 publications with Wnt5a and Embryonic Tissue
Brain Antibodies	>84 publications with Wnt5a and Brain
Skin Antibodies	>62 publications with Wnt5a and Skin
Lung Antibodies	>47 publications with Wnt5a and Lung
Muscle Antibodies	>47 publications with Wnt5a and Muscle
Bone Marrow Antibodies	>42 publications with Wnt5a and Bone Marrow
Nerve Antibodies	>40 publications with Wnt5a and Nerve
Connective Tissue Antibodies	>34 publications with Wnt5a and Connective Tissue
Uterus Antibodies	>31 publications with Wnt5a and Uterus