Rabbit Anti-FN1 Monoclonal Antibody – Cat# MBS179372

Western blot analysis of Fibronectin expression in HepG2 cell lysate (MBS179372).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FN1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FN1

Fibronectin (FN1) Antibody – Rabbit Monoclonal

Name: FN1 Antibody; Monoclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179372
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179372

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction… More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Fibronectin (FN1), Monoclonal Antibody

Full Product Name

Anti-Fibronectin Rabbit Monoclonal Antibody

Product Gene Name

anti-FN1 antibody

Product Synonym Names

Fibronectin; FN; Cold-insoluble globulin; CIG; Anastellin; Ugl-Y1; Ugl-Y2; Ugl-Y3; FN1; FN

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

262625 MW

OMIM

AJ276395 mRNA

3D Structure

ModBase 3D Structure for P02751

Immunogen

A synthesized peptide derived from human Fibronectin

Subcellular Localization

Secreted, extracellular space, extracellular matrix.

Tissue Specificity

Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-FN1 antibody

Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Application Notes for anti-FN1 antibody

WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200

Other Notes

Small volumes of anti-FN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FN1 purchase

MBS179372 is an antibody targeting FN1. Also known as Anti-Fibronectin Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for FN1. It may not necessarily be applicable to this product.

NCBI GI #

807066342

NCBI GeneID

2335

NCBI Accession #

NP_001293058.1

UniProt Primary Accession #

P02751

UniProt Secondary Accession #

O95609; O95610; Q14312; Q14325; Q14326; Q17RV7; Q564H7; Q585T2; B7ZLF0; E9PE77; E9PG29

UniProt Related Accession #

P02751

GenBank Nucleotide #

NM_001306129.1

NCBI Official Full Name

fibronectin isoform 8

NCBI Official Synonym Full Names

fibronectin 1

NCBI Official Symbol

FN1

NCBI Official Synonym Symbols

FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2; SMDCF

UniProt Protein Name

Fibronectin

UniProt Synonym Protein Names

Cold-insoluble globulin; CIG

Protein Family

Fibronectin

UniProt Gene Name

FN1

UniProt Synonym Gene Names

FN; FN; CIG

NCBI Protein Information

fibronectin

NCBI Summary for FN1

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016]

Research Articles on FN1

1. analysis of FN in breast cancer reveals its role and diagnostic potential

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-FN1 antibody

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Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies	Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Bacterial Invasion Of Epithelial Cells Pathway antibodies	Bacterial Invasion Of Epithelial Cells Pathway Diagram
Bacterial Invasion Of Epithelial Cells Pathway antibodies	Bacterial Invasion Of Epithelial Cells Pathway Diagram
Cell Surface Interactions At The Vascular Wall Pathway antibodies	Cell Surface Interactions At The Vascular Wall Pathway Diagram

Diseases associated with anti-FN1 antibody

Disease Name	Pubmed Publications
Carcinoma Antibodies	>83 publications with FN1 and Carcinoma
Fibrosis Antibodies	>38 publications with FN1 and Fibrosis
Inflammation Antibodies	>32 publications with FN1 and Inflammation
Lung Diseases Antibodies	>28 publications with FN1 and Lung Diseases
Cardiovascular Diseases Antibodies	>23 publications with FN1 and Cardiovascular Diseases
Lung Neoplasms Antibodies	>23 publications with FN1 and Lung Neoplasms
Breast Neoplasms Antibodies	>22 publications with FN1 and Breast Neoplasms
Neoplasms, Experimental Antibodies	>21 publications with FN1 and Neoplasms, Experimental
Neoplasm Invasiveness Antibodies	>19 publications with FN1 and Neoplasm Invasiveness
Kidney Diseases Antibodies	>17 publications with FN1 and Kidney Diseases

Organs/Tissues associated with anti-FN1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>66 publications with FN1 and Blood
Muscle Antibodies	>38 publications with FN1 and Muscle
Lung Antibodies	>37 publications with FN1 and Lung
Vascular Antibodies	>35 publications with FN1 and Vascular
Liver Antibodies	>30 publications with FN1 and Liver
Bone Antibodies	>30 publications with FN1 and Bone
Kidney Antibodies	>30 publications with FN1 and Kidney
Embryonic Tissue Antibodies	>23 publications with FN1 and Embryonic Tissue
Brain Antibodies	>21 publications with FN1 and Brain
Connective Tissue Antibodies	>21 publications with FN1 and Connective Tissue