Rabbit Anti-LOX Monoclonal Antibody – Cat# MBS179375

Western blot analysis of LOX expression in (1) Jurkat cell lysate; (2) Mouse brain lysate (MBS179375).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOX monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LOX

LOX Antibody – Rabbit Monoclonal

Name: LOX Antibody; Monoclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179375
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179375

Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression. More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

LOX, Monoclonal Antibody

Full Product Name

Anti-LOX Rabbit Monoclonal Antibody

Product Gene Name

anti-LOX antibody

Product Synonym Names

Protein-lysine 6-oxidase; 1.4.3.13; Lysyl oxidase; LOX

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

46944 MW

OMIM

S78694 mRNA

3D Structure

ModBase 3D Structure for P28300

Immunogen

A synthesized peptide derived from human LOX

Subcellular Localization

Secreted, extracellular space.

Tissue Specificity

Heart, placenta, skeletal muscle, kidney, lung and pancreas.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-LOX antibody

Signal Transduction; Cytoskeleton/ECM; Extracellular Matrix; ECM Enzymes; Other Enzymes; Cancer; Invasion/Microenvironment; Cardiovascular; Atherosclerosis; Vascular Inflammation; Leukocyte Recruitment; Vasculature; Endothelium; smooth muscle cell (smc)

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-LOX antibody

Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Application Notes for anti-LOX antibody

WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50
FC/FACS: 1:60

Other Notes

Small volumes of anti-LOX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LOX purchase

MBS179375 is an antibody targeting LOX. Also known as Anti-LOX Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for LOX. It may not necessarily be applicable to this product.

NCBI GI #

20149540

NCBI GeneID

4015

NCBI Accession #

NP_002308.2

UniProt Primary Accession #

P28300

UniProt Secondary Accession #

Q5FWF0; B2R5Q3

UniProt Related Accession #

P28300

GenBank Nucleotide #

NM_002317.6

NCBI Official Full Name

protein-lysine 6-oxidase isoform 1 preproprotein

NCBI Official Synonym Full Names

lysyl oxidase

NCBI Official Symbol

LOX

NCBI Official Synonym Symbols

AAT10

UniProt Protein Name

Protein-lysine 6-oxidase

UniProt Synonym Protein Names

Lysyl oxidase

Protein Family

Linoleate 9/13-lipoxygenase

UniProt Gene Name

LOX

NCBI Protein Information

protein-lysine 6-oxidase

NCBI Summary for LOX

This gene encodes a member of the lysyl oxidase family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate a regulatory propeptide and the mature enzyme. The copper-dependent amine oxidase activity of this enzyme functions in the crosslinking of collagens and elastin, while the propeptide may play a role in tumor suppression. In addition, defects in this gene have been linked with predisposition to thoracic aortic aneurysms and dissections. [provided by RefSeq, Jul 2016]

UniProt Comments for LOX

Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin (PubMed:26838787). Regulator of Ras expression. May play a role in tumor suppression. Plays a role in the aortic wall architecture ().

Research Articles on LOX

1. Our findings suggest that LOX has a role in cancer cell mitosis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-LOX antibody

Reference Product	PubMed Publications
LOXL1 antibody	>63 publications with LOX and LOXL1
SRC antibody	>37 publications with LOX and SRC
FGF2 antibody	>15 publications with LOX and FGF2
BMP1 antibody	>8 publications with LOX and BMP1
FBLN5 antibody	>8 publications with LOX and FBLN5
SNAI1 antibody	>2 publications with LOX and SNAI1
EFEMP2 antibody	>2 publications with LOX and EFEMP2

Pathways associated with anti-LOX antibody

Products by Pathway	Pathway Diagram
Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway antibodies	Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway Diagram
Collagen Formation Pathway antibodies	Collagen Formation Pathway Diagram
Crosslinking Of Collagen Fibrils Pathway antibodies	Crosslinking Of Collagen Fibrils Pathway Diagram
Elastic Fibre Formation Pathway antibodies	Elastic Fibre Formation Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram

Diseases associated with anti-LOX antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>672 publications with LOX and Inflammation
Cardiovascular Diseases Antibodies	>628 publications with LOX and Cardiovascular Diseases
Disease Models, Animal Antibodies	>424 publications with LOX and Disease Models, Animal
Necrosis Antibodies	>255 publications with LOX and Necrosis
Heart Diseases Antibodies	>194 publications with LOX and Heart Diseases
Lung Diseases Antibodies	>179 publications with LOX and Lung Diseases
Neoplasms, Experimental Antibodies	>158 publications with LOX and Neoplasms, Experimental
Hypertension Antibodies	>155 publications with LOX and Hypertension
Fibrosis Antibodies	>155 publications with LOX and Fibrosis
Lung Neoplasms Antibodies	>132 publications with LOX and Lung Neoplasms

Organs/Tissues associated with anti-LOX antibody

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>780 publications with LOX and Vascular
Muscle Antibodies	>426 publications with LOX and Muscle
Brain Antibodies	>398 publications with LOX and Brain
Lung Antibodies	>305 publications with LOX and Lung
Heart Antibodies	>290 publications with LOX and Heart
Liver Antibodies	>263 publications with LOX and Liver
Bone Antibodies	>250 publications with LOX and Bone
Skin Antibodies	>187 publications with LOX and Skin
Kidney Antibodies	>175 publications with LOX and Kidney
Embryonic Tissue Antibodies	>164 publications with LOX and Embryonic Tissue