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CD68 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179385

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage… More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD68/Sr D1, Monoclonal Antibody
Full Product Name
Anti-CD68/Sr D1 Rabbit Monoclonal Antibody
Product Gene Name
anti-CD68 antibody
Product Synonym Names
Macrosialin; Gp110; CD68; CD68
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
37408 MW
OMIM
S57235 mRNA
3D Structure
ModBase 3D Structure for P34810
Immunogen
A synthesized peptide derived from human CD68
Subcellular Localization
Isoform Short: Cell membrane; Single-pass type I membrane protein.
Tissue Specificity
Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-CD68 antibody

Immunology; Cell Type Markers; myeloid cells
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CD68 antibody
Western Blot (WB)
Application Notes for anti-CD68 antibody
WB: 1:500-1:1000

Western Blot (WB) of anti-CD68 antibody

Western blot analysis of CD68 expression in Jurkat cell lysate (MBS179385).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD68 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD68
Rabbit Anti-CD68 Monoclonal Antibody – Cat# MBS179385

Other Notes

Small volumes of anti-CD68 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD68 purchase

MBS179385 is an antibody targeting CD68. Also known as Anti-CD68/Sr D1 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CD68. It may not necessarily be applicable to this product.

NCBI GI #
91199550
NCBI GeneID
968
NCBI Accession #
NP_001035148.1
UniProt Primary Accession #
P34810
UniProt Secondary Accession #
Q53HR6; Q53XI3; Q96BI7; B4DVT4
UniProt Related Accession #
P34810
GenBank Nucleotide #
NM_001040059.1
NCBI Official Full Name
macrosialin isoform B
NCBI Official Synonym Full Names
CD68 molecule
NCBI Official Symbol
CD68
NCBI Official Synonym Symbols
GP110; LAMP4; SCARD1
UniProt Protein Name
Macrosialin
UniProt Synonym Protein Names
Gp110; CD_antigen: CD68
Protein Family
Macrosialin
UniProt Gene Name
CD68
NCBI Protein Information
macrosialin

NCBI Summary for CD68

This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]
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Research Articles on CD68

1. Taken together, these results suggested that the changes, including ox-LDL/LDL ratio, CD68(+)/RANK(+) cells number, and the levels of RANKL and HMGB1 in AS patients, favored osteoclastogenesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD68 antibody

 Reference Product  PubMed Publications
 CD4 antibody  >811 publications with CD68 and CD4
 CD34 antibody  >560 publications with CD68 and CD34
 CD163 antibody  >285 publications with CD68 and CD163
 TYROBP antibody  >2 publications with CD68 and TYROBP
 PSMD7 antibody  >1 publications with CD68 and PSMD7

Pathways associated with anti-CD68 antibody

 Products by Pathway  Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram

Diseases associated with anti-CD68 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >2027 publications with CD68 and Neoplasms
 Inflammation Antibodies  >1407 publications with CD68 and Inflammation
 Cardiovascular Diseases Antibodies  >824 publications with CD68 and Cardiovascular Diseases
 Necrosis Antibodies  >817 publications with CD68 and Necrosis
 Fibrosis Antibodies  >414 publications with CD68 and Fibrosis
 Disease Models, Animal Antibodies  >370 publications with CD68 and Disease Models, Animal
 Kidney Diseases Antibodies  >342 publications with CD68 and Kidney Diseases
 Atherosclerosis Antibodies  >309 publications with CD68 and Atherosclerosis
 Skin Neoplasms Antibodies  >292 publications with CD68 and Skin Neoplasms
 Adenocarcinoma Antibodies  >277 publications with CD68 and Adenocarcinoma

Organs/Tissues associated with anti-CD68 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2560 publications with CD68 and Blood
 Vascular Antibodies  >1243 publications with CD68 and Vascular
 Muscle Antibodies  >962 publications with CD68 and Muscle
 Skin Antibodies  >826 publications with CD68 and Skin
 Bone Antibodies  >764 publications with CD68 and Bone
 Brain Antibodies  >685 publications with CD68 and Brain
 Lymph Node Antibodies  >495 publications with CD68 and Lymph Node
 Liver Antibodies  >461 publications with CD68 and Liver
 Lung Antibodies  >456 publications with CD68 and Lung
 Kidney Antibodies  >437 publications with CD68 and Kidney
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