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HSP27 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179410

MBS179410 is an antibody targeting Hsp27. Also known as Anti-Hsp27 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. Involved in stress resistance and actin organization. More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Hsp27, Monoclonal Antibody
Full Product Name
Anti-Hsp27 Rabbit Monoclonal Antibody
Product Gene Name
anti-Hsp27 antibody
Product Synonym Gene Name
HSPB1
Product Synonym Names
Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27; HSPB1; HSP27; HSP28
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
22783 MW
OMIM
L39370 Genomic DNA
3D Structure
ModBase 3D Structure for P04792
Immunogen
A synthesized peptide derived from human Hsp27
Subcellular Localization
Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
Tissue Specificity
Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-Hsp27 antibody

Signal Transduction; Protein Trafficking; chaperones; Heat Shock Proteins; Cancer; Tumor Biomarkers; Cardiovascular; Atherosclerosis; Ischemia/Reperfusion; Heart; contractility; contractile proteins; actins
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-Hsp27 antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-Hsp27 antibody
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50
FC/FACS: 1:50

Western Blot (WB) of anti-Hsp27 antibody

Western blot analysis of Hsp27 expression in HeLa cell lysate (MBS179410).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPB1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HSPB1
Rabbit Anti-HSP27 Monoclonal Antibody – Cat# MBS179410

Other Notes

Small volumes of anti-Hsp27 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Hsp27. It may not necessarily be applicable to this product.

NCBI GI #
4504517
NCBI GeneID
3315
NCBI Accession #
NP_001531.1
UniProt Primary Accession #
P04792
UniProt Secondary Accession #
Q6FI47; Q96C20; Q96EI7; Q9UC31; Q9UC34; Q9UC35; Q9UC36; B2R4N8
UniProt Related Accession #
P04792
GenBank Nucleotide #
NM_001540.4
NCBI Official Full Name
heat shock protein beta-1
NCBI Official Synonym Full Names
heat shock protein family B (small) member 1
NCBI Official Symbol
HSPB1
NCBI Official Synonym Symbols
CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
UniProt Protein Name
Heat shock protein beta-1
UniProt Synonym Protein Names
28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27
Protein Family
Heat shock protein
UniProt Gene Name
HSPB1
UniProt Synonym Gene Names
HSP27; HSP28; HspB1; HSP 27; SRP27
NCBI Protein Information
heat shock protein beta-1

NCBI Summary for Hsp27

This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. [provided by RefSeq, Aug 2017]
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UniProt Comments for Hsp27

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).
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Research Articles on Hsp27

1. Apatorsen ( 2'-methoxyethyl-modified antisense oligonucleotide that inhibits Hsp27 expression)was tolerated at the highest dose evaluated (1000 mg). Single-agent activity was suggested by changes in tumor markers, circulating tumor cells , and stable measurable disease

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Hsp27 antibody

 Reference Product  PubMed Publications
 HSPB8 antibody  >51 publications with Hsp27 and HSPB8
 MAPKAPK2 antibody  >49 publications with Hsp27 and MAPKAPK2
 CRYAB antibody  >15 publications with Hsp27 and CRYAB
 MAPKAPK5 antibody  >5 publications with Hsp27 and MAPKAPK5
 TP53 antibody  >5 publications with Hsp27 and TP53
 DAXX antibody  >4 publications with Hsp27 and DAXX
 MAPKAPK3 antibody  >2 publications with Hsp27 and MAPKAPK3
 CRYAA antibody  >2 publications with Hsp27 and CRYAA
 NFKBIA antibody  >1 publications with Hsp27 and NFKBIA
 IGSF21 antibody  >1 publications with Hsp27 and IGSF21

Pathways associated with anti-Hsp27 antibody

 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Destabilization Of MRNA By AUF1 (hnRNP D0) Pathway antibodies  Destabilization Of MRNA By AUF1 (hnRNP D0) Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 FAS Pathway And Stress Induction Of HSP Regulation antibodies  FAS Pathway And Stress Induction Of HSP Regulation Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 IL-3 Signaling Pathway antibodies  IL-3 Signaling Pathway Diagram
 IL-6 Signaling Pathway antibodies  IL-6 Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram

Diseases associated with anti-Hsp27 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >281 publications with Hsp27 and Neoplasms
 Carcinoma Antibodies  >131 publications with Hsp27 and Carcinoma
 Cardiovascular Diseases Antibodies  >108 publications with Hsp27 and Cardiovascular Diseases
 Drug Toxicity Antibodies  >63 publications with Hsp27 and Drug Toxicity
 Inflammation Antibodies  >60 publications with Hsp27 and Inflammation
 Necrosis Antibodies  >59 publications with Hsp27 and Necrosis
 Breast Neoplasms Antibodies  >52 publications with Hsp27 and Breast Neoplasms
 Heart Diseases Antibodies  >52 publications with Hsp27 and Heart Diseases
 Liver Diseases Antibodies  >39 publications with Hsp27 and Liver Diseases
 Kidney Diseases Antibodies  >31 publications with Hsp27 and Kidney Diseases

Organs/Tissues associated with anti-Hsp27 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >196 publications with Hsp27 and Muscle
 Blood Antibodies  >141 publications with Hsp27 and Blood
 Brain Antibodies  >140 publications with Hsp27 and Brain
 Heart Antibodies  >106 publications with Hsp27 and Heart
 Nerve Antibodies  >98 publications with Hsp27 and Nerve
 Vascular Antibodies  >89 publications with Hsp27 and Vascular
 Liver Antibodies  >77 publications with Hsp27 and Liver
 Kidney Antibodies  >68 publications with Hsp27 and Kidney
 Lung Antibodies  >66 publications with Hsp27 and Lung
 Skin Antibodies  >43 publications with Hsp27 and Skin
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