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ROCK1 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179425

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility… More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ROCK1, Monoclonal Antibody
Full Product Name
Anti-ROCK1 Rabbit Monoclonal Antibody
Product Gene Name
anti-ROCK1 antibody
Product Synonym Names
Rho-associated protein kinase 1; 2.7.11.1; Renal carcinoma antigen NY-REN-35; Rho-associated, coiled-coil-containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase I; ROCK-I; p160 ROCK-1; p160ROCK; ROCK1
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
158175 MW
OMIM
U43195 mRNA
3D Structure
ModBase 3D Structure for Q13464
Immunogen
A synthesized peptide derived from human ROCK1
Subcellular Localization
Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Golgi apparatus membrane; Peripheral membrane protein. Cell projection, bleb. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, ruffle. Associated with the mother centriole and an intercentriolar linker. Colocalizes with ITGB1BP1 and ITGB1 at the cell membrane predominantly in lamellipodia and membrane ruffles, but also in retraction fibers. Localizes at the cell membrane in an ITGB1BP1-dependent manner (By similarity). A small proportion is associated with Golgi membranes.
Tissue Specificity
Detected in blood platelets.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-ROCK1 antibody

Signal Transduction; Cytoskeleton/ECM; Cytoskeleton; microfilaments; actin etc; actin assembly; Signal Transduction; Protein Phosphorylation; SER/THR Kinases; Other Kinases; Cell Biology; Cell Cycle; Cell Division; centromere; Cancer
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-ROCK1 antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-ROCK1 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50
FC/FACS: 1:50

Western Blot (WB) of anti-ROCK1 antibody

Western blot analysis of ROCK1 expression in Ramos cell lysate (MBS179425).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROCK1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ROCK1
Rabbit Anti-ROCK1 Monoclonal Antibody – Cat# MBS179425

Immunohistochemistry (IHC) of anti-ROCK1 antibody

Immunohistochemical analysis of paraffin-embedded human kidney, using ROCK1 Antibody(MBS179425)
ROCK1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ROCK1 Antibody (MBS179425)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-ROCK1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS179425

Other Notes

Small volumes of anti-ROCK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ROCK1 purchase

MBS179425 is an antibody targeting ROCK1. Also known as Anti-ROCK1 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ROCK1. It may not necessarily be applicable to this product.

NCBI GI #
4885583
NCBI GeneID
6093
NCBI Accession #
NP_005397.1
UniProt Primary Accession #
Q13464
UniProt Secondary Accession #
Q2KHM4; Q59GZ4; B0YJ91
UniProt Related Accession #
Q13464
GenBank Nucleotide #
NM_005406.2
NCBI Official Full Name
rho-associated protein kinase 1
NCBI Official Synonym Full Names
Rho associated coiled-coil containing protein kinase 1
NCBI Official Symbol
ROCK1
NCBI Official Synonym Symbols
ROCK-I; P160ROCK
UniProt Protein Name
Rho-associated protein kinase 1
UniProt Synonym Protein Names
Renal carcinoma antigen NY-REN-35; Rho-associated, coiled-coil-containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase I; ROCK-I; p160 ROCK-1; p160ROCK
Protein Family
Rho-associated protein kinase
UniProt Gene Name
ROCK1
UniProt Synonym Gene Names
ROCK-I; p160ROCK
NCBI Protein Information
rho-associated protein kinase 1

NCBI Summary for ROCK1

This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18. [provided by RefSeq, Aug 2015]
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Research Articles on ROCK1

1. Combined EGFR and ROCK inhibition effectively blocks proliferation of triple-negative breast cancer (TNBC) cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ROCK1 antibody

 Reference Product  PubMed Publications
 RHOA antibody  >112 publications with ROCK1 and RHOA
 RAC1 antibody  >14 publications with ROCK1 and RAC1
 RND3 antibody  >8 publications with ROCK1 and RND3
 LIMK2 antibody  >5 publications with ROCK1 and LIMK2
 CAV1 antibody  >2 publications with ROCK1 and CAV1
 PFN1 antibody  >1 publications with ROCK1 and PFN1

Pathways associated with anti-ROCK1 antibody

 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptotic Cleavage Of Cellular Proteins Pathway antibodies  Apoptotic Cleavage Of Cellular Proteins Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram

Diseases associated with anti-ROCK1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >75 publications with ROCK1 and Neoplasms
 Disease Models, Animal Antibodies  >41 publications with ROCK1 and Disease Models, Animal
 Hypertension Antibodies  >29 publications with ROCK1 and Hypertension
 Heart Diseases Antibodies  >26 publications with ROCK1 and Heart Diseases
 Inflammation Antibodies  >22 publications with ROCK1 and Inflammation
 Fibrosis Antibodies  >20 publications with ROCK1 and Fibrosis
 Diabetes Mellitus Antibodies  >18 publications with ROCK1 and Diabetes Mellitus
 Nervous System Diseases Antibodies  >16 publications with ROCK1 and Nervous System Diseases
 Lung Neoplasms Antibodies  >12 publications with ROCK1 and Lung Neoplasms
 Adenocarcinoma Antibodies  >11 publications with ROCK1 and Adenocarcinoma

Organs/Tissues associated with anti-ROCK1 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >76 publications with ROCK1 and Muscle
 Vascular Antibodies  >64 publications with ROCK1 and Vascular
 Blood Antibodies  >61 publications with ROCK1 and Blood
 Heart Antibodies  >44 publications with ROCK1 and Heart
 Lung Antibodies  >29 publications with ROCK1 and Lung
 Nerve Antibodies  >22 publications with ROCK1 and Nerve
 Brain Antibodies  >21 publications with ROCK1 and Brain
 Kidney Antibodies  >19 publications with ROCK1 and Kidney
 Bone Antibodies  >18 publications with ROCK1 and Bone
 Embryonic Tissue Antibodies  >16 publications with ROCK1 and Embryonic Tissue
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