Rabbit Anti-MRE11 Monoclonal Antibody – Cat# MBS179431

Western blot analysis of Mre11 expression in K562 cell lysate (MBS179431).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRE11A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MRE11A

MRE11 Antibody – Rabbit Monoclonal

Name: MRE11 Antibody; Monoclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179431
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179431

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA Recombinantion, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand- specific 3'-5'… More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Mre11, Monoclonal Antibody

Full Product Name

Anti-Mre11 Rabbit Monoclonal Antibody

Product Gene Name

anti-Mre11 antibody

Product Synonym Gene Name

MRE11A

Product Synonym Names

Double-strand break repair protein MRE11A; Meiotic Recombinantion 11 homolog 1; MRE11 homolog 1; Meiotic Recombinantion 11 homolog A; MRE11 homolog A; MRE11A; HNGS1; MRE11

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

80593 MW

OMIM

U37359 mRNA

3D Structure

ModBase 3D Structure for P49959

Immunogen

A synthesized peptide derived from human Mre11

Subcellular Localization

Nucleus. Chromosome, telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-Mre11 antibody

Immunohistochemistry (IHC), Western Blot (WB)

Application Notes for anti-Mre11 antibody

WB: 1:500-1:1000
IHC: 1:50-1:200

Other Notes

Small volumes of anti-Mre11 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Mre11 purchase

MBS179431 is an antibody targeting Mre11. Also known as Anti-Mre11 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Mre11. It may not necessarily be applicable to this product.

NCBI GI #

24234690

NCBI GeneID

4361

NCBI Accession #

NP_005581.2

UniProt Primary Accession #

P49959

UniProt Secondary Accession #

O43475; B3KTC7

UniProt Related Accession #

P49959

GenBank Nucleotide #

NM_005590.3

NCBI Official Full Name

double-strand break repair protein MRE11 isoform 2

NCBI Official Synonym Full Names

MRE11 homolog, double strand break repair nuclease

NCBI Official Symbol

MRE11

NCBI Official Synonym Symbols

ATLD; HNGS1; MRE11A; MRE11B

UniProt Protein Name

Double-strand break repair protein MRE11

UniProt Synonym Protein Names

Double-strand break repair protein MRE11A; Meiotic recombination 11 homolog 1; MRE11 homolog 1; Meiotic recombination 11 homolog A; MRE11 homolog A

Protein Family

DNA double-strand break repair protein

UniProt Gene Name

MRE11

UniProt Synonym Gene Names

MRE11 homolog 1; MRE11 homolog A

NCBI Protein Information

double-strand break repair protein MRE11; double-strand break repair protein MRE11A

NCBI Summary for Mre11

This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

UniProt Comments for Mre11

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547). The complex may also be required for DNA damage signaling via activation of the ATM kinase (PubMed:15064416). In telomeres the MRN complex may modulate t-loop formation (PubMed:10888888).

Research Articles on Mre11

1. Low MRE11 expression is associated with low-grade epithelial ovarian cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Mre11 antibody

Reference Product	PubMed Publications
RAD50 antibody	>274 publications with Mre11 and RAD50
NBN antibody	>194 publications with Mre11 and NBN
ATM antibody	>149 publications with Mre11 and ATM
H2AFX antibody	>41 publications with Mre11 and H2AFX
TP53 antibody	>12 publications with Mre11 and TP53
FANCD2 antibody	>7 publications with Mre11 and FANCD2
DCLRE1C antibody	>5 publications with Mre11 and DCLRE1C
XRCC6 antibody	>2 publications with Mre11 and XRCC6

Pathways associated with anti-Mre11 antibody

Products by Pathway	Pathway Diagram
Assembly Of The RAD50-MRE11-NBS1 Complex At DNA Double-strand Breaks Pathway antibodies	Assembly Of The RAD50-MRE11-NBS1 Complex At DNA Double-strand Breaks Pathway Diagram
BARD1 Signaling Events Pathway antibodies	BARD1 Signaling Events Pathway Diagram
BRCA1-associated Genome Surveillance Complex (BASC) Pathway antibodies	BRCA1-associated Genome Surveillance Complex (BASC) Pathway Diagram
DNA Repair Pathway antibodies	DNA Repair Pathway Diagram
DNA Damage Response Pathway antibodies	DNA Damage Response Pathway Diagram
Double-Strand Break Repair Pathway antibodies	Double-Strand Break Repair Pathway Diagram
Homologous Recombination Repair Pathway antibodies	Homologous Recombination Repair Pathway Diagram
Homologous Recombination Pathway antibodies	Homologous Recombination Pathway Diagram
Homologous Recombination Pathway antibodies	Homologous Recombination Pathway Diagram
Homologous Recombination Pathway antibodies	Homologous Recombination Pathway Diagram

Diseases associated with anti-Mre11 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>110 publications with Mre11 and Neoplasms
Nervous System Diseases Antibodies	>45 publications with Mre11 and Nervous System Diseases
Breast Neoplasms Antibodies	>29 publications with Mre11 and Breast Neoplasms
ATAXIA-TELANGIECTASIA-LIKE DISORDER Antibodies	>29 publications with Mre11 and ATAXIA-TELANGIECTASIA-LIKE DISORDER
Colorectal Neoplasms Antibodies	>21 publications with Mre11 and Colorectal Neoplasms
Congenital Abnormalities Antibodies	>13 publications with Mre11 and Congenital Abnormalities
Neoplasms, Experimental Antibodies	>11 publications with Mre11 and Neoplasms, Experimental
Ovarian Neoplasms Antibodies	>10 publications with Mre11 and Ovarian Neoplasms
Anemia Antibodies	>10 publications with Mre11 and Anemia
Cell Transformation, Neoplastic Antibodies	>9 publications with Mre11 and Cell Transformation, Neoplastic

Organs/Tissues associated with anti-Mre11 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>24 publications with Mre11 and Blood
Lung Antibodies	>17 publications with Mre11 and Lung
Brain Antibodies	>11 publications with Mre11 and Brain
Kidney Antibodies	>9 publications with Mre11 and Kidney
Prostate Antibodies	>6 publications with Mre11 and Prostate
Muscle Antibodies	>5 publications with Mre11 and Muscle
Testis Antibodies	>5 publications with Mre11 and Testis
Skin Antibodies	>5 publications with Mre11 and Skin
Liver Antibodies	>5 publications with Mre11 and Liver
Bladder Antibodies	>5 publications with Mre11 and Bladder