Rabbit Anti-Lin28B Monoclonal Antibody – Cat# MBS179485

Western blot analysis of Lin28B expression in K562 cell lysate (MBS179485).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIN28B monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LIN28B

Lin28B Antibody – Rabbit Monoclonal

Name: Lin28B Antibody; Monoclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179485
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179485

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Lin28B, Monoclonal Antibody

Full Product Name

Anti-Lin28B Rabbit Monoclonal Antibody

Product Gene Name

anti-Lin28B antibody

Product Synonym Names

Protein lin-28 homolog B; Lin-28B; LIN28B; CSDD2

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

27084 MW

OMIM

DQ127228 mRNA

3D Structure

ModBase 3D Structure for Q6ZN17

Immunogen

A synthesized peptide derived from human Lin28B

Subcellular Localization

Nucleus. Nucleus, nucleolus. Cytoplasm. Predominantly nucleolar (PubMed: 22118463). In Huh7 cells, predominantly cytoplasmic, with only a subset of cells exhibiting strong nuclear staining; however, the specificity of the polyclonal antibody used in these experiments has not been not documented (PubMed: 16971064).

Tissue Specificity

Expressed at high levels in the placenta and, at mucher lower, in testis and fetal liver (PubMed: 16971064). Isoform 1 is only detected in placenta and in moderately and poorly differentiated hepatocellular carcinoma cells (at protein level). Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues (at protein level). Tends to be up-regulated in triple-negative (ER-,PR-,HER2-) breast tumors, as well as in liver, ovarian, and thyroid carcinomas (PubMed: 22118463).

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-Lin28B antibody

Epigenetics and Nuclear Signaling; rnai; miRNA Processing; Cancer; Tumor Biomarkers

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-Lin28B antibody

Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)

Application Notes for anti-Lin28B antibody

WB: 1:500-1:2000
ICC/IF: 1:100-1:500
IP: 1:50
FC/FACS: 1:100

Other Notes

Small volumes of anti-Lin28B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Lin28B purchase

MBS179485 is an antibody targeting Lin28B. Also known as Anti-Lin28B Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Lin28B. It may not necessarily be applicable to this product.

NCBI GI #

51972214

NCBI GeneID

389421

NCBI Accession #

NP_001004317.1

UniProt Primary Accession #

Q6ZN17

UniProt Secondary Accession #

Q5TCM4; A1L165; B2RPN6

UniProt Related Accession #

Q6ZN17

GenBank Nucleotide #

NM_001004317.3

NCBI Official Full Name

protein lin-28 homolog B

NCBI Official Synonym Full Names

lin-28 homolog B

NCBI Official Symbol

LIN28B

NCBI Official Synonym Symbols

CSDD2

UniProt Protein Name

Protein lin-28 homolog B

Protein Family

Protein

UniProt Gene Name

LIN28B

UniProt Synonym Gene Names

CSDD2; Lin-28B

NCBI Protein Information

protein lin-28 homolog B

NCBI Summary for Lin28B

The protein encoded by this gene belongs to the lin-28 family, which is characterized by the presence of a cold-shock domain and a pair of CCHC zinc finger domains. This gene is highly expressed in testis, fetal liver, placenta, and in primary human tumors and cancer cell lines. It is negatively regulated by microRNAs that target sites in the 3' UTR, and overexpression of this gene in primary tumors is linked to the repression of let-7 family of microRNAs and derepression of let-7 targets, which facilitates cellular transformation. [provided by RefSeq, Jun 2012]

UniProt Comments for Lin28B

Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA (PubMed:22118463). Does not act on pri-miR21 (PubMed:22118463). The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation (PubMed:19703396). This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent (PubMed:22118463). Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression (). When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.

Research Articles on Lin28B

1. LIN28B is over-expressed in specific subtypes of pediatric leukemia and regulates lncRNA H19.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Lin28B antibody

Reference Product	PubMed Publications
MYC antibody	>18 publications with Lin28B and MYC
MAX antibody	>4 publications with Lin28B and MAX
ZCCHC11 antibody	>3 publications with Lin28B and ZCCHC11

Pathways associated with anti-Lin28B antibody

Products by Pathway	Pathway Diagram
Cardiac Progenitor Differentiation Pathway antibodies	Cardiac Progenitor Differentiation Pathway Diagram
Validated Targets Of C-MYC Transcriptional Activation Pathway antibodies	Validated Targets Of C-MYC Transcriptional Activation Pathway Diagram

Diseases associated with anti-Lin28B antibody

Disease Name	Pubmed Publications
Liver Diseases Antibodies	>11 publications with Lin28B and Liver Diseases
Neoplasm Metastasis Antibodies	>8 publications with Lin28B and Neoplasm Metastasis
Neuroblastoma Antibodies	>8 publications with Lin28B and Neuroblastoma
Cell Transformation, Neoplastic Antibodies	>8 publications with Lin28B and Cell Transformation, Neoplastic
Lung Neoplasms Antibodies	>6 publications with Lin28B and Lung Neoplasms
Inflammation Antibodies	>5 publications with Lin28B and Inflammation
Death Antibodies	>5 publications with Lin28B and Death
Fetal Diseases Antibodies	>3 publications with Lin28B and Fetal Diseases
Embryo Loss Antibodies	>2 publications with Lin28B and Embryo Loss
Birth Weight Antibodies	>2 publications with Lin28B and Birth Weight

Organs/Tissues associated with anti-Lin28B antibody

Organ/Tissue Name	Pubmed Publications
Embryonic Tissue Antibodies	>16 publications with Lin28B and Embryonic Tissue
Liver Antibodies	>13 publications with Lin28B and Liver
Kidney Antibodies	>4 publications with Lin28B and Kidney
Bone Antibodies	>3 publications with Lin28B and Bone
Testis Antibodies	>3 publications with Lin28B and Testis
Placenta Antibodies	>2 publications with Lin28B and Placenta
Nerve Antibodies	>1 publications with Lin28B and Nerve
Eye Antibodies	>1 publications with Lin28B and Eye
Skin Antibodies	>1 publications with Lin28B and Skin