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FASN Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179494

Anti-FASN/Fatty Acid Synthase Rabbit Monoclonal Antibody catalog # M00941. Tested in FC, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
FASN/Fatty Acid Synthase, Monoclonal Antibody
Full Product Name
Anti-FASN/Fatty Acid Synthase Rabbit Monoclonal Antibody
Product Gene Name
anti-FASN antibody
Product Synonym Names
Fatty acid synthase; 2.3.1.85; [Acyl-carrier-protein] S-acetyltransferase; 2.3.1.38; [Acyl-carrier-protein] S-malonyltransferase; 2.3.1.39; 3-oxoacyl-[acyl-carrier-protein] synthase; 2.3.1.41; 3-oxoacyl-[acyl-carrier-protein] reductase; 1.1.1.100; 3-hydroxyacyl-[acyl-carrier-protein] dehydratase; 4.2.1.59; Enoyl-[acyl-carrier-protein] reductase; 1.3.1.39; Oleoyl-[acyl-carrier-protein] hydrolase; 3.1.2.14; FASN; FAS
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
273427 MW
OMIM
U26644 mRNA
3D Structure
ModBase 3D Structure for P49327
Immunogen
A synthesized peptide derived from human FASN.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-FASN antibody

Cardiovascular; Lipids/Lipoproteins; Fatty Acids; Synthesis; Signal Transduction; Metabolism; Energy Metabolism; Neuroscience; Neurology Process; Lipid Metabolism; Cancer; Tumor Biomarkers; Enzymes; Cancer Metabolism; Metabolic Signaling Pathway; Metaboli
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-FASN antibody
Flow Cytometry (FC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-FASN antibody
Dilute the sample so that the expected range of concentrations fall within the detection range of this kit.
If the expected range of concentration is unknown, a pilot test should be conducted to decide the optimal dilution ratio for your samples.
Some PubMed article(s) citing the expression level of this target are as follows: Internal QC testing used
WB: 1:1000-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50
FC: 1:50

Western Blot (WB) of anti-FASN antibody

Western blot analysis of FASN expression in A549 cell lysate (MBS179494).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FASN monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FASN
Rabbit Anti-FASN Monoclonal Antibody – Cat# MBS179494

Other Notes

Small volumes of anti-FASN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FASN purchase

MBS179494 is an antibody targeting FASN. Also known as Anti-FASN/Fatty Acid Synthase Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for FASN. It may not necessarily be applicable to this product.

NCBI GI #
41872631
NCBI GeneID
2194
NCBI Accession #
NP_004095.4
UniProt Primary Accession #
P49327
UniProt Secondary Accession #
Q13479; Q16702; Q4LE83; Q6P4U5; Q6SS02; Q969R1; Q96C68; Q96IT0
UniProt Related Accession #
P49327
GenBank Nucleotide #
NM_004104.4
NCBI Official Full Name
fatty acid synthase
NCBI Official Synonym Full Names
fatty acid synthase
NCBI Official Symbol
FASN
NCBI Official Synonym Symbols
FAS; OA-519; SDR27X1
UniProt Protein Name
Fatty acid synthase
Protein Family
Fatty acid synthase
UniProt Gene Name
FASN
UniProt Synonym Gene Names
FAS
NCBI Protein Information
fatty acid synthase

NCBI Summary for FASN

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008]
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UniProt Comments for FASN

Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.

Research Articles on FASN

1. These findings implicate Spot14 as a direct protein enhancer of FASN catalysis in the mammary gland during lactation when maximal MCFA production is needed.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-FASN antibody

 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Activation Of Gene Expression By SREBP (SREBF) Pathway antibodies  Activation Of Gene Expression By SREBP (SREBF) Pathway Diagram
 ChREBP Activates Metabolic Gene Expression Pathway antibodies  ChREBP Activates Metabolic Gene Expression Pathway Diagram
 Fatty Acid Biosynthesis Pathway antibodies  Fatty Acid Biosynthesis Pathway Diagram
 Fatty Acyl-CoA Biosynthesis Pathway antibodies  Fatty Acyl-CoA Biosynthesis Pathway Diagram
 Fatty Acid Biosynthesis Pathway antibodies  Fatty Acid Biosynthesis Pathway Diagram
 Fatty Acid Biosynthesis Pathway antibodies  Fatty Acid Biosynthesis Pathway Diagram
 Fatty Acid Biosynthesis, Elongation Pathway antibodies  Fatty Acid Biosynthesis, Elongation Pathway Diagram
 Fatty Acid Biosynthesis, Elongation Pathway antibodies  Fatty Acid Biosynthesis, Elongation Pathway Diagram
 Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies  Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram

Diseases associated with anti-FASN antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >158 publications with FASN and Neoplasms
 Fatty Liver Antibodies  >81 publications with FASN and Fatty Liver
 Obesity Antibodies  >69 publications with FASN and Obesity
 Liver Diseases Antibodies  >36 publications with FASN and Liver Diseases
 Breast Neoplasms Antibodies  >36 publications with FASN and Breast Neoplasms
 Insulin Resistance Antibodies  >31 publications with FASN and Insulin Resistance
 Inflammation Antibodies  >25 publications with FASN and Inflammation
 Weight Loss Antibodies  >16 publications with FASN and Weight Loss
 Drug Toxicity Antibodies  >15 publications with FASN and Drug Toxicity
 Hepatitis Antibodies  >12 publications with FASN and Hepatitis

Organs/Tissues associated with anti-FASN antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >98 publications with FASN and Liver
 Adipose Tissue Antibodies  >73 publications with FASN and Adipose Tissue
 Blood Antibodies  >72 publications with FASN and Blood
 Connective Tissue Antibodies  >45 publications with FASN and Connective Tissue
 Muscle Antibodies  >34 publications with FASN and Muscle
 Prostate Antibodies  >34 publications with FASN and Prostate
 Mammary Gland Antibodies  >21 publications with FASN and Mammary Gland
 Lung Antibodies  >13 publications with FASN and Lung
 Brain Antibodies  >12 publications with FASN and Brain
 Kidney Antibodies  >10 publications with FASN and Kidney
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