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Alkaline Phosphatase (ALPL) Antibody – Rabbit Monoclonal DBF-1

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179518

Anti-Alkaline Phosphatase ALPL Rabbit Monoclonal Antibody catalog # M01008-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Alkaline Phosphatase (ALPL), Monoclonal DBF-1 Antibody
Full Product Name
Anti-Alkaline Phosphatase Rabbit Monoclonal Antibody
Product Gene Name
anti-ALPL antibody
Product Synonym Names
Alkaline phosphatase, tissue-nonspecific isozyme; AP-TNAP; TNSALP; 3.1.3.1; Alkaline phosphatase liver/bone/kidney isozyme; ALPL
Clonality
Monoclonal DBF-1
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Concentration
0.5 mg/mL (lot specific)
Preparation and Storage
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
3D Structure
ModBase 3D Structure for P05186
Immunogen
A synthesized peptide derived from human Alkaline Phosphatase
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-ALPL antibody

Cell Biology; Cell Cycle; Kinases/Phosphatases; Tags & Cell Markers; fusion/marker proteins; alkaline phosphatase; Cancer; Tumor Biomarkers; Enzymes
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-ALPL antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-ALPL antibody
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
FC 1:50

Western Blot (WB) of anti-ALPL antibody

Western blot analysis of ALPL using anti-ALPL antibody (MBS179518).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat spleen tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALPL antigen affinity purified monoclonal antibody (Catalog # MBS179518) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for ALPL at approximately 80 kDa. The expected band size for ALPL is at 57 kDa.
Rabbit Anti-ALPL Monoclonal DBF-1 Antibody – Cat# MBS179518

Immunohystochemistry (IHC) of anti-ALPL antibody

Immunohistochemical analysis of paraffin-embedded human liver, using Alkaline Phosphatase Antibody.
Rabbit Anti-ALPL Monoclonal DBF-1 Antibody, Immunohystochemistry (IHC) – Cat# MBS179518

Immunofluorescence (IF) of anti-ALPL antibody

Immunofluorescent analysis of Hela cells, using Alkaline Phosphatase Antibody .
Rabbit Anti-ALPL Monoclonal DBF-1 Antibody, Immunofluorescence (IF) – Cat# MBS179518

Other Notes

Small volumes of anti-ALPL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ALPL purchase

MBS179518 is an antibody targeting ALPL. Also known as Anti-Alkaline Phosphatase Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ALPL. It may not necessarily be applicable to this product.

NCBI GI #
116734717
NCBI GeneID
249
NCBI Accession #
NP_000469.3
UniProt Primary Accession #
P05186
UniProt Secondary Accession #
O75090; Q2TAI7; Q59EJ7; Q5BKZ5; Q5VTG5; Q6NZI8; Q8WU32; A1A4E7; B2RMP8; B7Z387; B7Z4Y6
UniProt Related Accession #
P05186
GenBank Nucleotide #
NM_000478.5
NCBI Official Full Name
alkaline phosphatase, tissue-nonspecific isozyme isoform 1 preproprotein
NCBI Official Synonym Full Names
alkaline phosphatase, liver/bone/kidney
NCBI Official Symbol
ALPL
NCBI Official Synonym Symbols
HOPS; TNAP; APTNAP; TNSALP; AP-TNAP
UniProt Protein Name
Alkaline phosphatase, tissue-nonspecific isozyme
UniProt Synonym Protein Names
Alkaline phosphatase liver/bone/kidney isozyme
Protein Family
Alkaline phosphatase
UniProt Gene Name
ALPL
UniProt Synonym Gene Names
AP-TNAP; TNSALP
NCBI Protein Information
alkaline phosphatase, tissue-nonspecific isozyme

NCBI Summary for ALPL

This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects. [provided by RefSeq, Oct 2015]
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UniProt Comments for ALPL

This isozyme may play a role in skeletal mineralization.

Research Articles on ALPL

1. These results show that an increase of TNAP activity in ACDC (arterial calcification due to deficiency of CD73) contributes to ectopic calcification by disrupting the extracellular balance of PPi and Pi and identify potential therapeutic targets for ACDC.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ALPL antibody

 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 Folate Biosynthesis Pathway antibodies  Folate Biosynthesis Pathway Diagram
 Folate Biosynthesis Pathway antibodies  Folate Biosynthesis Pathway Diagram
 TNF-alpha/NF-kB Signaling Pathway antibodies  TNF-alpha/NF-kB Signaling Pathway Diagram

Diseases associated with anti-ALPL antibody

 Disease Name  Pubmed Publications
 Hypophosphatasia, Adult Antibodies  >17 publications with ALPL and Hypophosphatasia, Adult
 Neoplasms Antibodies  >14 publications with ALPL and Neoplasms
 Kidney Diseases Antibodies  >9 publications with ALPL and Kidney Diseases
 Hypophosphatasia, Infantile Antibodies  >9 publications with ALPL and Hypophosphatasia, Infantile
 Hypophosphatasia, Childhood Antibodies  >8 publications with ALPL and Hypophosphatasia, Childhood
 Cardiovascular Diseases Antibodies  >8 publications with ALPL and Cardiovascular Diseases
 Seizures Antibodies  >6 publications with ALPL and Seizures
 Pain Antibodies  >6 publications with ALPL and Pain
 Calcinosis Antibodies  >5 publications with ALPL and Calcinosis
 Hypertension Antibodies  >4 publications with ALPL and Hypertension

Organs/Tissues associated with anti-ALPL antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >123 publications with ALPL and Bone
 Blood Antibodies  >42 publications with ALPL and Blood
 Kidney Antibodies  >35 publications with ALPL and Kidney
 Liver Antibodies  >34 publications with ALPL and Liver
 Connective Tissue Antibodies  >28 publications with ALPL and Connective Tissue
 Brain Antibodies  >11 publications with ALPL and Brain
 Mouth Antibodies  >10 publications with ALPL and Mouth
 Adipose Tissue Antibodies  >10 publications with ALPL and Adipose Tissue
 Embryonic Tissue Antibodies  >6 publications with ALPL and Embryonic Tissue
 Skin Antibodies  >6 publications with ALPL and Skin
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