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C9 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179519

Anti-C9/Complement Component C9 Rabbit Monoclonal Antibody catalog # MBS179519. Tested in IP, IHC, WB applications. This antibody reacts with Human. More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
C9/Complement Component C9, Monoclonal Antibody
Full Product Name
Anti-C9/Complement Component C9 Rabbit Monoclonal Antibody
Product Gene Name
anti-C9 antibody
Clonality
Monoclonal
Isotype
IgG
Clone Number
BAO-3
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/ml BSA.
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. For short term storage and frequent use, store at 4 degree C for up to one month. Avoid repeated freeze-thaw cycles
OMIM
X02176 mRNA
3D Structure
ModBase 3D Structure for P02748
Immunogen
A synthesized peptide derived from human C9
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-C9 antibody
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-C9 antibody
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50

Immunohistochemistry (IHC) of anti-C9 antibody

Immunohistochemical analysis of paraffin-embedded human kidney, using C9 Antibody.
Rabbit Anti-C9 Monoclonal Antibody – Cat# MBS179519

Western Blot (WB) of anti-C9 antibody

Western blot analysis of C9 expression in Human fetal liver lysate.
Rabbit Anti-C9 Monoclonal Antibody, Western Blot (WB) – Cat# MBS179519

Western Blot (WB) of anti-C9 antibody

Western blot analysis of C9 using anti-C9 antibody (MBS179519).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C9 antigen affinity purified monoclonal antibody (Catalog # MBS179519) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for C9 at approximately 70 kDa. The expected band size for C9 is at 63,70 kDa
Rabbit Anti-C9 Monoclonal Antibody, Western Blot (WB) – Cat# MBS179519

Other Notes

Small volumes of anti-C9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for C9 purchase

MBS179519 is an antibody targeting C9. Also known as Anti-C9/Complement Component C9 Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for C9. It may not necessarily be applicable to this product.

NCBI GI #
4502511
NCBI GeneID
735
NCBI Accession #
NP_001728.1
UniProt Primary Accession #
P02748
UniProt Related Accession #
P02748
GenBank Nucleotide #
NM_001737.4
NCBI Official Full Name
complement component C9 preproprotein
NCBI Official Synonym Full Names
complement C9
NCBI Official Symbol
C9
NCBI Official Synonym Symbols
C9D; ARMD15
UniProt Protein Name
Complement component C9
Protein Family
Complement component
UniProt Gene Name
C9
NCBI Protein Information
complement component C9

NCBI Summary for C9

This gene encodes the final component of the complement system. It participates in the formation of the Membrane Attack Complex (MAC). The MAC assembles on bacterial membranes to form a pore, permitting disruption of bacterial membrane organization. Mutations in this gene cause component C9 deficiency. [provided by RefSeq, Feb 2009]
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UniProt Comments for C9

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells (PubMed:9634479, PubMed:9212048, PubMed:26841934). C9 is the pore-forming subunit of the MAC (PubMed:4055801, PubMed:26841934).

Research Articles on C9

1. Serum-expressed apolipoprotein B-100 protein, C9 Complement, and gelsolin can be used for differential diagnosis of Barrertts esophagus and adenocarcinoma of esophagus.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-C9 antibody

 Reference Product  PubMed Publications
 CD59 antibody  >150 publications with C9 and CD59
 CLU antibody  >15 publications with C9 and CLU
 C8A antibody  >7 publications with C9 and C8A

Pathways associated with anti-C9 antibody

 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Complement Activation, Classical Pathway antibodies  Complement Activation, Classical Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement Cascade Pathway antibodies  Complement Cascade Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Prion Diseases Pathway antibodies  Prion Diseases Pathway Diagram

Diseases associated with anti-C9 antibody

 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >127 publications with C9 and Kidney Diseases
 Death Antibodies  >109 publications with C9 and Death
 Drug Toxicity Antibodies  >84 publications with C9 and Drug Toxicity
 Necrosis Antibodies  >82 publications with C9 and Necrosis
 Brain Diseases Antibodies  >71 publications with C9 and Brain Diseases
 Fatty Liver Antibodies  >54 publications with C9 and Fatty Liver
 Liver Diseases Antibodies  >46 publications with C9 and Liver Diseases
 Weight Loss Antibodies  >33 publications with C9 and Weight Loss
 Immunologic Deficiency Syndromes Antibodies  >28 publications with C9 and Immunologic Deficiency Syndromes
 Liver Neoplasms Antibodies  >21 publications with C9 and Liver Neoplasms

Organs/Tissues associated with anti-C9 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >233 publications with C9 and Liver
 Muscle Antibodies  >167 publications with C9 and Muscle
 Kidney Antibodies  >166 publications with C9 and Kidney
 Placenta Antibodies  >12 publications with C9 and Placenta
 Pancreas Antibodies  >12 publications with C9 and Pancreas
 Uncharacterized Tissue Antibodies  >1 publications with C9 and Uncharacterized Tissue
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