Rabbit Anti-FOXA2 Monoclonal Antibody – Cat# MBS179530

Western blot analysis of FOXA2 expression in HepG2 cell lysate (MBS179530).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXA2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FOXA2

FOXA2 Antibody – Rabbit Monoclonal

Name: FOXA2 Antibody; Monoclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179530
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179530

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

FOXA2/Hnf3B, Monoclonal Antibody

Full Product Name

Anti-FOXA2/Hnf3B Rabbit Monoclonal Antibody

Product Gene Name

anti-FOXA2 antibody

Product Synonym Names

Hepatocyte nuclear factor 3-beta; HNF-3-beta; HNF-3B; Forkhead box protein A2; Transcription factor 3B; TCF-3B; FOXA2; HNF3B; TCF3B

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

48306 MW

OMIM

AB028021 mRNA

3D Structure

ModBase 3D Structure for Q9Y261

Immunogen

A synthesized peptide derived from human FOXA2

Subcellular Localization

Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner; in response to insulin signaling via AKT1 is exported from the nucleus.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-FOXA2 antibody

Epigenetics and Nuclear Signaling; Transcription; Domain Families; Forkhead Box; foxa; Stem Cells; Lineage Markers; Endoderm; Developmental Biology; Lineage Specification; Metabolism; Types of Disease; Obesity

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-FOXA2 antibody

Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)

Application Notes for anti-FOXA2 antibody

WB: 1:500-1:1000
ICC/IF: 1:50-1:200

Other Notes

Small volumes of anti-FOXA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FOXA2 purchase

MBS179530 is an antibody targeting FOXA2. Also known as Anti-FOXA2/Hnf3B Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for FOXA2. It may not necessarily be applicable to this product.

NCBI GI #

194394143

NCBI GeneID

3170

NCBI Accession #

NP_068556.2

UniProt Primary Accession #

Q9Y261

UniProt Secondary Accession #

Q8WUW4; Q96DF7

UniProt Related Accession #

Q9Y261

GenBank Nucleotide #

NM_021784.4

NCBI Official Full Name

hepatocyte nuclear factor 3-beta isoform 1

NCBI Official Synonym Full Names

forkhead box A2

NCBI Official Symbol

FOXA2

NCBI Official Synonym Symbols

HNF3B; TCF3B

UniProt Protein Name

Hepatocyte nuclear factor 3-beta

UniProt Synonym Protein Names

Forkhead box protein A2; Transcription factor 3B; TCF-3B

Protein Family

Forkhead box protein

UniProt Gene Name

FOXA2

UniProt Synonym Gene Names

HNF3B; TCF3B; HNF-3-beta; HNF-3B; TCF-3B

NCBI Protein Information

hepatocyte nuclear factor 3-beta

NCBI Summary for FOXA2

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Oct 2008]

UniProt Comments for FOXA2

Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.

Research Articles on FOXA2

1. Results showed that FOXA2 expression was decreased during Il-13 induced goblet cell differentiation but DNA methylation of its promoter was not changed. In the absence of Il-13, no difference was found for FOXA2 expression but hypomethylation of CpG # 10 and 11 in the its promoter was detected. These findings suggest that aberrant DNA methylation of FOXA2 is one of the factors underlying mucus hypersecretion in COPD.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-FOXA2 antibody

Reference Product	PubMed Publications
FOXA1 antibody	>148 publications with FOXA2 and FOXA1
ONECUT1 antibody	>24 publications with FOXA2 and ONECUT1
NKX2-2 antibody	>20 publications with FOXA2 and NKX2-2
OTX2 antibody	>14 publications with FOXA2 and OTX2
INS antibody	>14 publications with FOXA2 and INS
GLI1 antibody	>11 publications with FOXA2 and GLI1
IGFBP1 antibody	>8 publications with FOXA2 and IGFBP1
NKX2-1 antibody	>6 publications with FOXA2 and NKX2-1
AKT1 antibody	>2 publications with FOXA2 and AKT1
GCG antibody	>2 publications with FOXA2 and GCG

Pathways associated with anti-FOXA2 antibody

Products by Pathway	Pathway Diagram
Cardiac Progenitor Differentiation Pathway antibodies	Cardiac Progenitor Differentiation Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
FOXA Transcription Factor Networks Pathway antibodies	FOXA Transcription Factor Networks Pathway Diagram
FOXA1 Transcription Factor Network Pathway antibodies	FOXA1 Transcription Factor Network Pathway Diagram
FOXA2 And FOXA3 Transcription Factor Networks Pathway antibodies	FOXA2 And FOXA3 Transcription Factor Networks Pathway Diagram
Heart Development Pathway antibodies	Heart Development Pathway Diagram
Hedgehog Signaling Events Mediated By Gli Proteins Pathway antibodies	Hedgehog Signaling Events Mediated By Gli Proteins Pathway Diagram
Maturity Onset Diabetes Of The Young Pathway antibodies	Maturity Onset Diabetes Of The Young Pathway Diagram
Maturity Onset Diabetes Of The Young Pathway antibodies	Maturity Onset Diabetes Of The Young Pathway Diagram
Regulation Of Beta-cell Development Pathway antibodies	Regulation Of Beta-cell Development Pathway Diagram

Diseases associated with anti-FOXA2 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>88 publications with FOXA2 and Neoplasms
Liver Diseases Antibodies	>67 publications with FOXA2 and Liver Diseases
Disease Models, Animal Antibodies	>37 publications with FOXA2 and Disease Models, Animal
Lung Diseases Antibodies	>30 publications with FOXA2 and Lung Diseases
Carcinoma, Hepatocellular Antibodies	>29 publications with FOXA2 and Carcinoma, Hepatocellular
Inflammation Antibodies	>19 publications with FOXA2 and Inflammation
Nervous System Diseases Antibodies	>18 publications with FOXA2 and Nervous System Diseases
Congenital Abnormalities Antibodies	>16 publications with FOXA2 and Congenital Abnormalities
Fatty Liver Antibodies	>15 publications with FOXA2 and Fatty Liver
Neoplasms, Experimental Antibodies	>14 publications with FOXA2 and Neoplasms, Experimental

Organs/Tissues associated with anti-FOXA2 antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>235 publications with FOXA2 and Liver
Embryonic Tissue Antibodies	>138 publications with FOXA2 and Embryonic Tissue
Pancreas Antibodies	>129 publications with FOXA2 and Pancreas
Lung Antibodies	>85 publications with FOXA2 and Lung
Intestine Antibodies	>40 publications with FOXA2 and Intestine
Eye Antibodies	>20 publications with FOXA2 and Eye
Kidney Antibodies	>18 publications with FOXA2 and Kidney
Uterus Antibodies	>7 publications with FOXA2 and Uterus
Ovary Antibodies	>5 publications with FOXA2 and Ovary
Connective Tissue Antibodies	>4 publications with FOXA2 and Connective Tissue