• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

YB1 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179538

Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and… More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
YB1, Monoclonal Antibody
Full Product Name
Anti-YB1 Rabbit Monoclonal Antibody
Product Gene Name
anti-YB1 antibody
Product Synonym Gene Name
YBX1
Product Synonym Names
Nuclease-sensitive element-binding protein 1; CCAAT-binding transcription factor I subunit A; CBF-A; DNA-binding protein B; DBPB; Enhancer factor I subunit A; EFI-A; Y-box transcription factor; Y-box-binding protein 1; YB-1; YBX1; NSEP1; YB1
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
35924 MW
OMIM
M24070 mRNA
3D Structure
ModBase 3D Structure for P67809
Immunogen
A synthesized peptide derived from human YB1
Subcellular Localization
Cytoplasm. Nucleus. Cytoplasmic granule. Secreted. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between nucleus and cytoplasm. Predominantly cytoplasmic in proliferating cells. Cytotoxic stress and DNA damage enhance translocation to the nucleus. Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress. Secreted by mesangial and monocytic cells after inflammatory challenges. Translocates from the cytoplasm to the nucleus after and colocalizes with APEX1 in nuclear speckles after genotoxic stress.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-YB1 antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-YB1 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50

Immunohistochemistry (IHC) of anti-YB1 antibody

Immunohistochemical analysis of paraffin-embedded human kidney, using YB1 Antibody(MBS179538)
YBX1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-YBX1 Antibody (MBS179538)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-YB1 Monoclonal Antibody – Cat# MBS179538

Western Blot (WB) of anti-YB1 antibody

Western blot analysis of YB1 expression in HeLa cell lysate (MBS179538).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YBX1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for YBX1
Rabbit Anti-YB1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS179538

Other Notes

Small volumes of anti-YB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for YB1 purchase

MBS179538 is an antibody targeting YB1. Also known as Anti-YB1 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for YB1. It may not necessarily be applicable to this product.

NCBI GI #
34098946
NCBI GeneID
4904
NCBI Accession #
NP_004550.2
UniProt Primary Accession #
P67809
UniProt Secondary Accession #
P16990; P16991; Q14972; Q15325; Q5FVF0
UniProt Related Accession #
P67809
GenBank Nucleotide #
NM_004559.4
NCBI Official Full Name
nuclease-sensitive element-binding protein 1
NCBI Official Synonym Full Names
Y-box binding protein 1
NCBI Official Symbol
YBX1
NCBI Official Synonym Symbols
YB1; BP-8; CSDB; DBPB; YB-1; CBF-A; CSDA2; EFI-A; NSEP1; NSEP-1; MDR-NF1
UniProt Protein Name
Nuclease-sensitive element-binding protein 1
UniProt Synonym Protein Names
CCAAT-binding transcription factor I subunit A; CBF-A; DNA-binding protein B; DBPB; Enhancer factor I subunit A; EFI-A; Y-box transcription factor; Y-box-binding protein 1; YB-1
UniProt Gene Name
YBX1
UniProt Synonym Gene Names
NSEP1; YB1; CBF-A; DBPB; EFI-A; YB-1
NCBI Protein Information
nuclease-sensitive element-binding protein 1

NCBI Summary for YB1

This gene encodes a highly conserved cold shock domain protein that has broad nucleic acid binding properties. The encoded protein functions as both a DNA and RNA binding protein and has been implicated in numerous cellular processes including regulation of transcription and translation, pre-mRNA splicing, DNA reparation and mRNA packaging. This protein is also a component of messenger ribonucleoprotein (mRNP) complexes and may have a role in microRNA processing. This protein can be secreted through non-classical pathways and functions as an extracellular mitogen. Aberrant expression of the gene is associated with cancer proliferation in numerous tissues. This gene may be a prognostic marker for poor outcome and drug resistance in certain cancers. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on multiple chromosomes. [provided by RefSeq, Sep 2015]
Show more

UniProt Comments for YB1

Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (). Regulates the transcription of numerous genes. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability. Binds preferentially to the 5'-[CU]CUGCG-3' motif in vitro.
Show more

Research Articles on YB1

1. YB-1 localization was significantly altered in the nuclei of tumor blasts derived from bone marrow or peripheral blood.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-YB1 antibody

 Reference Product  PubMed Publications
 TFAP2A antibody  >7 publications with YB1 and TFAP2A
 tat antibody  >5 publications with YB1 and tat
 PCNA antibody  >4 publications with YB1 and PCNA
 PURA antibody  >4 publications with YB1 and PURA
 SMAD3 antibody  >2 publications with YB1 and SMAD3
 FUS antibody  >2 publications with YB1 and FUS
 TP53 antibody  >2 publications with YB1 and TP53
 CTCF antibody  >2 publications with YB1 and CTCF
 SLC6A4 antibody  >1 publications with YB1 and SLC6A4
 NFYB antibody  >1 publications with YB1 and NFYB

Pathways associated with anti-YB1 antibody

 Products by Pathway  Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies  Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
 SIDS Susceptibility Pathways antibodies  SIDS Susceptibility Pathways Diagram
 Signaling Events Mediated By PTP1B Pathway antibodies  Signaling Events Mediated By PTP1B Pathway Diagram
 MRNA Splicing Pathway antibodies  MRNA Splicing Pathway Diagram
 MRNA Splicing - Major Pathway antibodies  MRNA Splicing - Major Pathway Diagram
 MRNA Processing Pathway antibodies  MRNA Processing Pathway Diagram

Diseases associated with anti-YB1 antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >43 publications with YB1 and Breast Neoplasms
 Nervous System Diseases Antibodies  >15 publications with YB1 and Nervous System Diseases
 Liver Diseases Antibodies  >15 publications with YB1 and Liver Diseases
 Immune System Diseases Antibodies  >12 publications with YB1 and Immune System Diseases
 Liver Neoplasms Antibodies  >10 publications with YB1 and Liver Neoplasms
 Disease Models, Animal Antibodies  >9 publications with YB1 and Disease Models, Animal
 Neoplasm Metastasis Antibodies  >8 publications with YB1 and Neoplasm Metastasis
 Lung Diseases Antibodies  >8 publications with YB1 and Lung Diseases
 Kidney Diseases Antibodies  >7 publications with YB1 and Kidney Diseases
 Carcinoma, Hepatocellular Antibodies  >6 publications with YB1 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-YB1 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >83 publications with YB1 and Liver
 Brain Antibodies  >36 publications with YB1 and Brain
 Blood Antibodies  >29 publications with YB1 and Blood
 Lung Antibodies  >26 publications with YB1 and Lung
 Kidney Antibodies  >26 publications with YB1 and Kidney
 Vascular Antibodies  >16 publications with YB1 and Vascular
 Muscle Antibodies  >16 publications with YB1 and Muscle
 Thyroid Antibodies  >13 publications with YB1 and Thyroid
 Mammary Gland Antibodies  >11 publications with YB1 and Mammary Gland
 Embryonic Tissue Antibodies  >11 publications with YB1 and Embryonic Tissue
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000