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CD40L Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179562

Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD40L, Monoclonal Antibody
Full Product Name
Anti-CD40L Rabbit Monoclonal Antibody
Product Gene Name
anti-CD40L antibody
Product Synonym Gene Name
CD40LG
Product Synonym Names
CD40 ligand; CD40-L; T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5; CD154; CD40 ligand, membrane form; CD40 ligand, soluble form; CD40LG; CD40L; TNFSF5; TRAP
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
29274 MW
OMIM
X68550 mRNA
3D Structure
ModBase 3D Structure for P29965
Immunogen
A synthesized peptide derived from human CD40L
Subcellular Localization
Cell membrane; Single-pass type II membrane protein.
Tissue Specificity
Specifically expressed on activated CD4+ T- lymphocytes.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-CD40L antibody

Immunology; Adaptive Immunity; T Cells; Immunology; immunoGlobulins; class switching; Innate Immunity; Cytokines; TNF Superfamily; Cardiovascular; Atherosclerosis; Thrombosis; PlatelETS; Vascular Inflammation; innate and Adaptive Immunity; Regulatory T Ce
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CD40L antibody
Western Blot (WB)
Application Notes for anti-CD40L antibody
WB: 1:500-1:2000

Western Blot (WB) of anti-CD40L antibody

Western blot analysis of CD40L expression in MOLT-4 cell lysate (MBS179562).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40LG monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD40LG
Rabbit Anti-CD40L Monoclonal Antibody – Cat# MBS179562

Other Notes

Small volumes of anti-CD40L antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD40L purchase

MBS179562 is an antibody targeting CD40L. Also known as Anti-CD40L Rabbit Monoclonal Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CD40L. It may not necessarily be applicable to this product.

NCBI GI #
4557433
NCBI GeneID
959
NCBI Accession #
NP_000065.1
UniProt Primary Accession #
P29965
UniProt Related Accession #
P29965
GenBank Nucleotide #
NM_000074.2
NCBI Official Full Name
CD40 ligand
NCBI Official Synonym Full Names
CD40 ligand
NCBI Official Symbol
CD40LG
NCBI Official Synonym Symbols
IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
UniProt Protein Name
CD40 ligand
UniProt Synonym Protein Names
T-cell antigen Gp39; TNF-related activation protein; TRAP
Protein Family
Tumor necrosis factor receptor superfamily
UniProt Gene Name
CD40LG
UniProt Synonym Gene Names
CD40L; TNFSF5; TRAP; CD40-L; TRAP
NCBI Protein Information
CD40 ligand

NCBI Summary for CD40L

The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]

UniProt Comments for CD40L

Cytokine that binds to CD40/TNFRSF5 (PubMed:1280226). Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933). Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching ().
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Research Articles on CD40L

1. Analysis of the early events after receptor engagement revealed that both TNF and CD40L activate the classical NF-kappaB pathway, and confirm activation of the alternative by the latter. Furthermore, using genetic and pharmacological inhibition of the classical pathway we show that activation of the alternative occurs independently of the former. This reveals insights into NF-kappaB signaling by CD40L and TNF in endoth...
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD40L antibody

 Reference Product  PubMed Publications
 CD40 antibody  >20 publications with CD40L and CD40
 ICAM1 antibody  >3 publications with CD40L and ICAM1
 CD86 antibody  >3 publications with CD40L and CD86
 TRAF1 antibody  >2 publications with CD40L and TRAF1
 RELA antibody  >1 publications with CD40L and RELA

Pathways associated with anti-CD40L antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 CD40/CD40L Signaling Pathway antibodies  CD40/CD40L Signaling Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Calcium Signaling In The CD4+ TCR Pathway antibodies  Calcium Signaling In The CD4+ TCR Pathway Diagram

Diseases associated with anti-CD40L antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >9 publications with CD40L and Inflammation
 Neoplasms Antibodies  >7 publications with CD40L and Neoplasms
 Necrosis Antibodies  >6 publications with CD40L and Necrosis
 Myocardial Infarction Antibodies  >3 publications with CD40L and Myocardial Infarction
 IMMUNODEFICIENCY WITH HYPER-IgM, TYPE 1 Antibodies  >3 publications with CD40L and IMMUNODEFICIENCY WITH HYPER-IgM, TYPE 1
 Myocardial Ischemia Antibodies  >3 publications with CD40L and Myocardial Ischemia
 Cardiovascular Diseases Antibodies  >3 publications with CD40L and Cardiovascular Diseases
 Heart Diseases Antibodies  >3 publications with CD40L and Heart Diseases
 Kidney Diseases Antibodies  >2 publications with CD40L and Kidney Diseases
 Hypersensitivity Antibodies  >2 publications with CD40L and Hypersensitivity

Organs/Tissues associated with anti-CD40L antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >25 publications with CD40L and Blood
 Mixed Antibodies  >1 publications with CD40L and Mixed
 Thymus Antibodies  >1 publications with CD40L and Thymus
 Brain Antibodies  >1 publications with CD40L and Brain
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