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SERCA2 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179578

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform 2 is involved in the regulation of the contraction/relaxation cycle. More details

$210 / 0.03 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
SERCA2, Monoclonal Antibody
Full Product Name
Anti-SERCA2 Rabbit Monoclonal Antibody
Product Gene Name
anti-SERCA2 antibody
Product Synonym Gene Name
ATP2A2
Product Synonym Names
Sarcoplasmic/endoplasmic reticulum calcium ATPase 2; SERCA2; SR Ca(2+)-ATPase 2; 3.6.3.8; Calcium pump 2; Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; ATP2A2; ATP2B
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
114757 MW
OMIM
M23114 mRNA
3D Structure
ModBase 3D Structure for P16615
Immunogen
A synthesized peptide derived from human SERCA2
Subcellular Localization
Endoplasmic reticulum membrane; Multi-pass membrane protein. Sarcoplasmic reticulum membrane; Multi-pass membrane protein.
Tissue Specificity
Isoform 1 is widely expressed in smooth muscle and nonmuscle tissues such as in adult skin epidermis, with highest expression in liver, pancreas and lung, and intermediate expression in brain, kidney and placenta. Also expressed at lower levels in heart and skeletal muscle. Isoforms 2 and 3 are highly expressed in the heart and slow twitch skeletal muscle. Expression of isoform 3 is predominantly restricted to cardiomyocytes and in close proximity to the sarcolemma. Both isoforms are mildly expressed in lung, kidney, liver, pancreas and placenta. Expression of isoform 3 is amplified during monocytic differentiation and also observed in the fetal heart.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-SERCA2 antibody

Signal Transduction; Signaling Pathway; Calcium Signaling; calcium channels; Signal Transduction; Metabolism; plasma membrane; atpases; Vitamins/Minerals; Cardiovascular; Heart; contractility; inotropics; Metabolism; Pathways and Processes; Cofactors; Vit
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-SERCA2 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-SERCA2 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
FC/FACS: 1:50

Western Blot (WB) of anti-SERCA2 antibody

Western blot analysis of SERCA2 expression in HeLa cell lysate (MBS179578).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP2A2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ATP2A2
Rabbit Anti-SERCA2 Monoclonal Antibody – Cat# MBS179578

Other Notes

Small volumes of anti-SERCA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SERCA2 purchase

MBS179578 is an antibody targeting SERCA2. Also known as Anti-SERCA2 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SERCA2. It may not necessarily be applicable to this product.

NCBI GI #
4502285
NCBI GeneID
488
NCBI Accession #
NP_001672.1
UniProt Primary Accession #
P16615
UniProt Secondary Accession #
P16614; Q86VJ2; A6NDN7; B4DF05
UniProt Related Accession #
P16615
GenBank Nucleotide #
NM_001681.3
NCBI Official Full Name
sarcoplasmic/endoplasmic reticulum calcium ATPase 2 isoform a
NCBI Official Synonym Full Names
ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2
NCBI Official Symbol
ATP2A2
NCBI Official Synonym Symbols
DD; DAR; ATP2B; SERCA2
UniProt Protein Name
Sarcoplasmic/endoplasmic reticulum calcium ATPase 2
UniProt Synonym Protein Names
Calcium pump 2; Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca(2+) ATPase
Protein Family
Sarcoplasmic/endoplasmic reticulum calcium ATPase
UniProt Gene Name
ATP2A2
UniProt Synonym Gene Names
ATP2B; SERCA2; SR Ca(2+)-ATPase 2
NCBI Protein Information
sarcoplasmic/endoplasmic reticulum calcium ATPase 2

NCBI Summary for SERCA2

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2008]
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UniProt Comments for SERCA2

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform 2 is involved in the regulation of the contraction/relaxation cycle (PubMed:16402920). Acts as a regulator of TNFSF11-mediated Ca2+ signaling pathways via its interaction with TMEM64 which is critical for the TNFSF11-induced CREB1 activation and mitochondrial ROS generation necessary for proper osteoclast generation. Association between TMEM64 and SERCA2 in the ER leads to cytosolic Ca (2+) spiking for activation of NFATC1 and production of mitochondrial ROS, thereby triggering Ca (2+) signaling cascades that promote osteoclast differentiation and activation ().
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Research Articles on SERCA2

1. Studies indicate that Darier disease (DD) is caused by mutations in the ATP2A2 gene, whereas the ATP2C1 gene is associated with Hailey-Hailey disease (HHD).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SERCA2 antibody

 Reference Product  PubMed Publications
 PLN antibody  >16 publications with SERCA2 and PLN
 SP1 antibody  >7 publications with SERCA2 and SP1
 CALU antibody  >3 publications with SERCA2 and CALU
 HAX1 antibody  >2 publications with SERCA2 and HAX1
 SLN antibody  >2 publications with SERCA2 and SLN
 IL6 antibody  >1 publications with SERCA2 and IL6

Pathways associated with anti-SERCA2 antibody

 Products by Pathway  Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Calcium Regulation In The Cardiac Cell Pathway antibodies  Calcium Regulation In The Cardiac Cell Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram

Diseases associated with anti-SERCA2 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >172 publications with SERCA2 and Cardiovascular Diseases
 Heart Diseases Antibodies  >161 publications with SERCA2 and Heart Diseases
 Darier Disease Antibodies  >114 publications with SERCA2 and Darier Disease
 Heart Failure Antibodies  >97 publications with SERCA2 and Heart Failure
 Hypertrophy Antibodies  >76 publications with SERCA2 and Hypertrophy
 Disease Models, Animal Antibodies  >65 publications with SERCA2 and Disease Models, Animal
 Cardiomegaly Antibodies  >51 publications with SERCA2 and Cardiomegaly
 Cardiomyopathies Antibodies  >48 publications with SERCA2 and Cardiomyopathies
 Ventricular Dysfunction, Left Antibodies  >43 publications with SERCA2 and Ventricular Dysfunction, Left
 Myocardial Infarction Antibodies  >26 publications with SERCA2 and Myocardial Infarction

Organs/Tissues associated with anti-SERCA2 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >269 publications with SERCA2 and Muscle
 Heart Antibodies  >256 publications with SERCA2 and Heart
 Blood Antibodies  >77 publications with SERCA2 and Blood
 Skin Antibodies  >71 publications with SERCA2 and Skin
 Vascular Antibodies  >31 publications with SERCA2 and Vascular
 Brain Antibodies  >20 publications with SERCA2 and Brain
 Lung Antibodies  >13 publications with SERCA2 and Lung
 Thyroid Antibodies  >13 publications with SERCA2 and Thyroid
 Embryonic Tissue Antibodies  >12 publications with SERCA2 and Embryonic Tissue
 Kidney Antibodies  >10 publications with SERCA2 and Kidney
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