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HIF-2 alpha Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179608

Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
HIF-2 alpha, Monoclonal Antibody
Full Product Name
Anti-HIF-2 alpha Rabbit Monoclonal Antibody
Product Gene Name
anti-HIF-2 alpha antibody
Product Synonym Gene Name
EPAS1
Product Synonym Names
Endothelial PAS domain-containing protein 1; EPAS-1; Basic-helix-loop-helix-PAS protein MOP2; Class E basic helix-loop-helix protein 73; bHLHe73; HIF-1-alpha-like factor; HLF; Hypoxia-inducible factor 2-alpha; HIF-2-alpha; HIF2-alpha; Member of PAS protein 2; PAS domain-containing protein 2; EPAS1; BHLHE73; HIF2A; MOP2; PASD2
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
96459 MW
OMIM
U81984 mRNA
3D Structure
ModBase 3D Structure for Q99814
Immunogen
A synthesized peptide derived from human HIF-2 alpha
Subcellular Localization
Nucleus. Nucleus speckle. Colocalizes with HIF3A in the nucleus and speckles.
Tissue Specificity
Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-HIF-2 alpha antibody

Cardiovascular; Hypoxia; HIF; Epigenetics and Nuclear Signaling; Transcription; Domain Families; HLH/Leucine Zipper; HLH; Cardiovascular/Immune; Cancer; Invasion/Microenvironment; ChIP'ing Antibodies; Cancer Metabolism; Response to Hypoxia; Metabolism; Pa
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-HIF-2 alpha antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-HIF-2 alpha antibody
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
FC/FACS: 1:50

Western Blot (WB) of anti-HIF-2 alpha antibody

Western blot analysis of HIF-2 alpha in (1)MCF-7 cell lysate;(2)HeLa cell lysate (MBS179608).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPAS1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for EPAS1
Rabbit Anti-HIF-2 alpha Monoclonal Antibody – Cat# MBS179608

Other Notes

Small volumes of anti-HIF-2 alpha antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HIF-2 alpha purchase

MBS179608 is an antibody targeting HIF-2 alpha. Also known as Anti-HIF-2 alpha Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HIF-2 alpha. It may not necessarily be applicable to this product.

NCBI GI #
40254439
NCBI GeneID
2034
NCBI Accession #
NP_001421.2
UniProt Primary Accession #
Q99814
UniProt Secondary Accession #
Q86VA2; Q99630
UniProt Related Accession #
Q99814
GenBank Nucleotide #
NM_001430.4
NCBI Official Full Name
endothelial PAS domain-containing protein 1
NCBI Official Synonym Full Names
endothelial PAS domain protein 1
NCBI Official Symbol
EPAS1
NCBI Official Synonym Symbols
HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73
UniProt Protein Name
Endothelial PAS domain-containing protein 1
UniProt Synonym Protein Names
Basic-helix-loop-helix-PAS protein MOP2; Class E basic helix-loop-helix protein 73; bHLHe73; HIF-1-alpha-like factor; HLF; Hypoxia-inducible factor 2-alpha; HIF-2-alpha; HIF2-alpha; Member of PAS protein 2; PAS domain-containing protein 2
Protein Family
Endothelial PAS domain-containing protein
UniProt Gene Name
EPAS1
UniProt Synonym Gene Names
BHLHE73; HIF2A; MOP2; PASD2; EPAS-1; bHLHe73; HLF; HIF-2-alpha; HIF2-alpha
NCBI Protein Information
endothelial PAS domain-containing protein 1

NCBI Summary for HIF-2 alpha

This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]
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UniProt Comments for HIF-2 alpha

Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.
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Research Articles on HIF-2 alpha

1. Thus, we provide evidence here that HIF-2a is a critical regulator of PD-L1 at both mRNA and protein levels and that HIF-2a regulates the expression of PD-L1 by binding directly to the HRE-4 in the PD-L1 proximal promoter.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HIF-2 alpha antibody

 Reference Product  PubMed Publications
 EGLN1 antibody  >10 publications with HIF-2 alpha and EGLN1
 ARNT antibody  >9 publications with HIF-2 alpha and ARNT
 VHL antibody  >9 publications with HIF-2 alpha and VHL
 VEGFA antibody  >6 publications with HIF-2 alpha and VEGFA
 FLT1 antibody  >5 publications with HIF-2 alpha and FLT1
 ARNT2 antibody  >2 publications with HIF-2 alpha and ARNT2
 UBC antibody  >1 publications with HIF-2 alpha and UBC

Pathways associated with anti-HIF-2 alpha antibody

 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Cellular Response To Hypoxia Pathway antibodies  Cellular Response To Hypoxia Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 HIF-2-alpha Transcription Factor Network Pathway antibodies  HIF-2-alpha Transcription Factor Network Pathway Diagram
 Oxygen-dependent Asparagine Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway antibodies  Oxygen-dependent Asparagine Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway Diagram
 Oxygen-dependent Proline Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway antibodies  Oxygen-dependent Proline Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram
 Regulation Of Gene Expression By Hypoxia-inducible Factor Pathway antibodies  Regulation Of Gene Expression By Hypoxia-inducible Factor Pathway Diagram
 Regulation Of Hypoxia-inducible Factor (HIF) By Oxygen Pathway antibodies  Regulation Of Hypoxia-inducible Factor (HIF) By Oxygen Pathway Diagram
 Renal Cell Carcinoma Pathway antibodies  Renal Cell Carcinoma Pathway Diagram

Diseases associated with anti-HIF-2 alpha antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >24 publications with HIF-2 alpha and Neoplasms
 Disease Models, Animal Antibodies  >7 publications with HIF-2 alpha and Disease Models, Animal
 Carcinoma, Renal Cell Antibodies  >7 publications with HIF-2 alpha and Carcinoma, Renal Cell
 Cardiovascular Diseases Antibodies  >6 publications with HIF-2 alpha and Cardiovascular Diseases
 Necrosis Antibodies  >6 publications with HIF-2 alpha and Necrosis
 Kidney Diseases Antibodies  >5 publications with HIF-2 alpha and Kidney Diseases
 Inflammation Antibodies  >5 publications with HIF-2 alpha and Inflammation
 Hypertrophy Antibodies  >4 publications with HIF-2 alpha and Hypertrophy
 Liver Diseases Antibodies  >3 publications with HIF-2 alpha and Liver Diseases
 Liver Neoplasms Antibodies  >3 publications with HIF-2 alpha and Liver Neoplasms

Organs/Tissues associated with anti-HIF-2 alpha antibody

 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >31 publications with HIF-2 alpha and Vascular
 Blood Antibodies  >21 publications with HIF-2 alpha and Blood
 Kidney Antibodies  >12 publications with HIF-2 alpha and Kidney
 Liver Antibodies  >11 publications with HIF-2 alpha and Liver
 Muscle Antibodies  >10 publications with HIF-2 alpha and Muscle
 Heart Antibodies  >10 publications with HIF-2 alpha and Heart
 Lung Antibodies  >9 publications with HIF-2 alpha and Lung
 Embryonic Tissue Antibodies  >9 publications with HIF-2 alpha and Embryonic Tissue
 Bone Antibodies  >7 publications with HIF-2 alpha and Bone
 Connective Tissue Antibodies  >6 publications with HIF-2 alpha and Connective Tissue
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