Rabbit Anti-CHGA Monoclonal Antibody – Cat# MBS179612

Western blot analysis of Chromogranin A expression in SH-SY5Y cell lysate (MBS179612).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHGA monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CHGA

Chromogranin A (CHGA) Antibody – Rabbit Monoclonal

Name: CHGA Antibody; Monoclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179612
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179612

Pancreastatin: Strongly inhibits glucose induced insulin release from the pancreas. More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Chromogranin A (CHGA), Monoclonal Antibody

Full Product Name

Anti-Chromogranin A Rabbit Monoclonal Antibody

Product Gene Name

anti-CHGA antibody

Product Synonym Names

Chromogranin-A; CgA; Pituitary secretory protein I; SP-I; Vasostatin-1; Vasostatin I; Vasostatin-2; Vasostatin II; EA-92; ES-43; Pancreastatin; SS-18; WA-8; WE-14; LF-19; Catestatin; SL21; AL-11; GV-19; GR-44; ER-37; GE-25; Serpinin-RRG; Serpinin; p-Glu serpinin precursor; CHGA

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

50688 MW

OMIM

J03483 mRNA

3D Structure

ModBase 3D Structure for P10645

Immunogen

A synthesized peptide derived from human Chromogranin A

Subcellular Localization

Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle membrane. Secreted. Associated with the secretory granule membrane through direct interaction to SCG3 that in turn binds to cholesterol-enriched lipid rafts in intragranular conditions.

Tissue Specificity

GE-25 is found in the brain.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-CHGA antibody

Neuroscience; Cell Type Marker; neuron marker; soma marker; Tags & Cell Markers; Cell Type Markers; Tumor Associated; Signal Transduction; Growth Factors/Hormones; insulin/insulin-like; Cancer; Growth Factors; insulin and insulin-like; Tumor Biomarkers

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-CHGA antibody

Flow Cytometry (FC/FACS), Immunocytochemistry (ICC)

Application Notes for anti-CHGA antibody

WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
FC/FACS: 1:20

Other Notes

Small volumes of anti-CHGA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CHGA purchase

MBS179612 is an antibody targeting CHGA. Also known as Anti-Chromogranin A Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CHGA. It may not necessarily be applicable to this product.

NCBI GI #

4502805

NCBI GeneID

1113

NCBI Accession #

NP_001266.1

UniProt Primary Accession #

P10645

UniProt Secondary Accession #

Q53FA8; Q6NR84; Q96E84; Q96GL7; Q9BQB5; B2R9E9

UniProt Related Accession #

P10645

GenBank Nucleotide #

NM_001275.3

NCBI Official Full Name

chromogranin-A isoform 1 preproprotein

NCBI Official Synonym Full Names

chromogranin A

NCBI Official Symbol

CHGA

NCBI Official Synonym Symbols

CGA

UniProt Protein Name

Chromogranin-A

UniProt Synonym Protein Names

SL21

Protein Family

Chromogranin

UniProt Gene Name

CHGA

UniProt Synonym Gene Names

CgA; SP-I

NCBI Protein Information

chromogranin-A

NCBI Summary for CHGA

The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

UniProt Comments for CHGA

Pancreastatin: Strongly inhibits glucose induced insulin release from the pancreas.Catestatin: Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist (PubMed:15326220). Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa (PubMed:15723172 and PubMed:24723458). Can induce mast cell migration, degranulation and production of cytokines and chemokines (PubMed:21214543). Acts as a potent scavenger of free radicals in vitro (PubMed:24723458). May play a role in the regulation of cardiac function and blood pressure (PubMed:18541522).

Research Articles on CHGA

1. Results show that chronic lymphocytic leukemia (CLL) patients had increased plasma levels of chromogranin A (CgA), compared to normal subjects, particularly those >70-year-old or those treated with proton pump inhibitors.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CHGA antibody

Reference Product	PubMed Publications
SYP antibody	>6 publications with CHGA and SYP
SCG2 antibody	>3 publications with CHGA and SCG2
CALM1 antibody	>1 publications with CHGA and CALM1
IGF1 antibody	>1 publications with CHGA and IGF1
SCG3 antibody	>1 publications with CHGA and SCG3

Diseases associated with anti-CHGA antibody

Disease Name	Pubmed Publications
Pheochromocytoma Antibodies	>62 publications with CHGA and Pheochromocytoma
Hypertension Antibodies	>54 publications with CHGA and Hypertension
Cardiovascular Diseases Antibodies	>49 publications with CHGA and Cardiovascular Diseases
Nervous System Diseases Antibodies	>45 publications with CHGA and Nervous System Diseases
Brain Diseases Antibodies	>34 publications with CHGA and Brain Diseases
Hyperplasia Antibodies	>28 publications with CHGA and Hyperplasia
Kidney Diseases Antibodies	>24 publications with CHGA and Kidney Diseases
Carcinoma, Non-Small-Cell Lung Antibodies	>22 publications with CHGA and Carcinoma, Non-Small-Cell Lung
Liver Diseases Antibodies	>18 publications with CHGA and Liver Diseases
Necrosis Antibodies	>10 publications with CHGA and Necrosis

Organs/Tissues associated with anti-CHGA antibody

Organ/Tissue Name	Pubmed Publications
Nerve Antibodies	>102 publications with CHGA and Nerve
Adrenal Gland Antibodies	>79 publications with CHGA and Adrenal Gland
Prostate Antibodies	>53 publications with CHGA and Prostate
Lung Antibodies	>48 publications with CHGA and Lung
Stomach Antibodies	>47 publications with CHGA and Stomach
Brain Antibodies	>45 publications with CHGA and Brain
Thyroid Antibodies	>42 publications with CHGA and Thyroid
Pancreas Antibodies	>32 publications with CHGA and Pancreas
Parathyroid Antibodies	>28 publications with CHGA and Parathyroid
Kidney Antibodies	>25 publications with CHGA and Kidney