Rabbit Anti-Cdc25C Monoclonal Antibody – Cat# MBS179634

Western blot analysis of Cdc25C expression in K562 cell lysate (MBS179634).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC25C monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDC25C

Cdc25C Antibody – Rabbit Monoclonal

Name: Cdc25C Antibody; Monoclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179634
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179634

Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its… More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Cdc25C, Monoclonal Antibody

Full Product Name

Anti-Cdc25C Rabbit Monoclonal Antibody

Product Gene Name

anti-Cdc25C antibody

Product Synonym Names

M-phase inducer phosphatase 3; 3.1.3.48; Dual specificity phosphatase Cdc25C; CDC25C

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

53365 MW

OMIM

M34065 mRNA

3D Structure

ModBase 3D Structure for P30307

Immunogen

A synthesized peptide derived from human Cdc25C

Subcellular Localization

Nucleus.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-Cdc25C antibody

Cell Biology; Cell Cycle; Kinases/Phosphatases; Epigenetics and Nuclear Signaling; phospatases

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-Cdc25C antibody

Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Application Notes for anti-Cdc25C antibody

WB: 1:500-1:2000
IHC: 1:10-1:500
ICC/IF: 1:50-1:200
IP: 1:30
FC/FACS: 1:50

Other Notes

Small volumes of anti-Cdc25C antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Cdc25C purchase

MBS179634 is an antibody targeting Cdc25C. Also known as Anti-Cdc25C Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Cdc25C. It may not necessarily be applicable to this product.

NCBI GI #

567316253

NCBI GeneID

995

NCBI Accession #

NP_001274511.1

UniProt Primary Accession #

P30307

UniProt Secondary Accession #

Q96PL3; Q9H168; Q9H2E8; Q9H2E9; Q9H2F1; D3DQB8

UniProt Related Accession #

P30307

GenBank Nucleotide #

NM_001287582.1

NCBI Official Full Name

M-phase inducer phosphatase 3 isoform a

NCBI Official Synonym Full Names

cell division cycle 25C

NCBI Official Symbol

CDC25C

NCBI Official Synonym Symbols

CDC25; PPP1R60

UniProt Protein Name

M-phase inducer phosphatase 3

UniProt Synonym Protein Names

Dual specificity phosphatase Cdc25C

UniProt Gene Name

CDC25C

NCBI Protein Information

M-phase inducer phosphatase 3

NCBI Summary for Cdc25C

This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Dec 2015]

Research Articles on Cdc25C

1. Mdm2 overexpression and Cdc25C downregulation delay cell cycle progression through the G2/M phase.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Cdc25C antibody

Reference Product	PubMed Publications
CCNB1 antibody	>335 publications with Cdc25C and CCNB1
CDK1 antibody	>209 publications with Cdc25C and CDK1
CHEK1 antibody	>102 publications with Cdc25C and CHEK1
CHEK2 antibody	>83 publications with Cdc25C and CHEK2
CDK2 antibody	>79 publications with Cdc25C and CDK2
PLK1 antibody	>71 publications with Cdc25C and PLK1
MARK3 antibody	>9 publications with Cdc25C and MARK3
PLK3 antibody	>9 publications with Cdc25C and PLK3
YWHAB antibody	>2 publications with Cdc25C and YWHAB

Pathways associated with anti-Cdc25C antibody

Products by Pathway	Pathway Diagram
Activation Of ATR In Response To Replication Stress Pathway antibodies	Activation Of ATR In Response To Replication Stress Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Checkpoints Pathway antibodies	Cell Cycle Checkpoints Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle - G2/M Transition Pathway antibodies	Cell Cycle - G2/M Transition Pathway Diagram
Cell Cycle - G2/M Transition Pathway antibodies	Cell Cycle - G2/M Transition Pathway Diagram
Chk1/Chk2(Cds1) Mediated Inactivation Of Cyclin B:Cdk1 Complex Pathway antibodies	Chk1/Chk2(Cds1) Mediated Inactivation Of Cyclin B:Cdk1 Complex Pathway Diagram

Diseases associated with anti-Cdc25C antibody

Disease Name	Pubmed Publications
Carcinoma Antibodies	>187 publications with Cdc25C and Carcinoma
Adenocarcinoma Antibodies	>66 publications with Cdc25C and Adenocarcinoma
Breast Neoplasms Antibodies	>61 publications with Cdc25C and Breast Neoplasms
Lung Neoplasms Antibodies	>61 publications with Cdc25C and Lung Neoplasms
Prostatic Neoplasms Antibodies	>45 publications with Cdc25C and Prostatic Neoplasms
Liver Neoplasms Antibodies	>40 publications with Cdc25C and Liver Neoplasms
Liver Diseases Antibodies	>40 publications with Cdc25C and Liver Diseases
Carcinoma, Hepatocellular Antibodies	>39 publications with Cdc25C and Carcinoma, Hepatocellular
Neoplasms, Experimental Antibodies	>36 publications with Cdc25C and Neoplasms, Experimental
Colonic Neoplasms Antibodies	>32 publications with Cdc25C and Colonic Neoplasms

Organs/Tissues associated with anti-Cdc25C antibody

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>83 publications with Cdc25C and Lung
Prostate Antibodies	>59 publications with Cdc25C and Prostate
Liver Antibodies	>55 publications with Cdc25C and Liver
Blood Antibodies	>42 publications with Cdc25C and Blood
Brain Antibodies	>27 publications with Cdc25C and Brain
Bone Antibodies	>21 publications with Cdc25C and Bone
Skin Antibodies	>18 publications with Cdc25C and Skin
Testis Antibodies	>10 publications with Cdc25C and Testis
Ovary Antibodies	>6 publications with Cdc25C and Ovary
Intestine Antibodies	>6 publications with Cdc25C and Intestine