Rabbit Anti-SAA1 Monoclonal Antibody – Cat# MBS179642

Western blot analysis of Serum Amyloid A expression in human plasma lysate (MBS179642).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAA1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SAA1

Serum Amyloid A (SAA1) Antibody – Rabbit Monoclonal

Name: SAA1 Antibody; Monoclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179642
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179642

MBS179642 is an antibody targeting SAA1. Also known as Anti-Serum Amyloid A Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit. Major acute phase protein. More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Serum Amyloid A (SAA1), Monoclonal Antibody

Full Product Name

Anti-Serum Amyloid A Rabbit Monoclonal Antibody

Product Gene Name

anti-SAA1 antibody

Product Synonym Names

Serum amyloid A-1 protein; SAA; Amyloid protein A; Amyloid fibril protein AA; Serum amyloid protein A(2-104); Serum amyloid protein A(3-104); Serum amyloid protein A(2-103); Serum amyloid protein A(2-102); Serum amyloid protein A(4-101); SAA1

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

13532 MW

OMIM

M10906 mRNA

3D Structure

ModBase 3D Structure for P0DJI8

Immunogen

A synthesized peptide derived from human Serum Amyloid A

Subcellular Localization

Secreted.

Tissue Specificity

Expressed by the liver; secreted in plasma (at protein level).

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-SAA1 antibody

Immunology; Innate Immunity; Macrophage/Inflamm.; Immunology; complement; Regulatory; Signal Transduction; Signaling Pathway; G Protein Signaling; gpcr; Cytokines; Cardiovascular; Lipids/Lipoproteins; lipoproteins/apolipoproteins

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-SAA1 antibody

Immunohistochemistry (IHC), Western Blot (WB)

Application Notes for anti-SAA1 antibody

WB: 1:500-1:2000
IHC: 1:50-1:200

Other Notes

Small volumes of anti-SAA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SAA1. It may not necessarily be applicable to this product.

NCBI GI #

40316912

NCBI GeneID

6288

NCBI Accession #

NP_000322.2

UniProt Primary Accession #

P0DJI8

UniProt Secondary Accession #

P02735; P02736; P02737; Q16730; Q16834; Q16835; Q16879; Q3KRB3; Q6FG67; Q96QN0; Q9UCK9

UniProt Related Accession #

P0DJI8

GenBank Nucleotide #

NM_000331.5

NCBI Official Full Name

serum amyloid A-1 protein preproprotein

NCBI Official Synonym Full Names

serum amyloid A1

NCBI Official Symbol

SAA1

NCBI Official Synonym Symbols

SAA; PIG4; SAA2; TP53I4

UniProt Protein Name

Serum amyloid A-1 protein

UniProt Synonym Protein Names

Amyloid protein AAlternative name(s):Amyloid fibril protein AA

Protein Family

Amyloid protein

UniProt Gene Name

SAA1

UniProt Synonym Gene Names

SAA

NCBI Protein Information

serum amyloid A-1 protein

NCBI Summary for SAA1

This gene encodes a member of the serum amyloid A family of apolipoproteins. The encoded preproprotein is proteolytically processed to generate the mature protein. This protein is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors. Alternate splicing results in multiple transcript variants that encode the same protein. A pseudogene of this gene is found on chromosome 11. [provided by RefSeq, Feb 2016]

UniProt Comments for SAA1

Major acute phase protein.

Research Articles on SAA1

1. SAA1 can be a potential mediator for UV-induced MMP-1 expression in human skin.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-SAA1 antibody

Products by Pathway	Pathway Diagram
Activated TLR4 Signalling Pathway antibodies	Activated TLR4 Signalling Pathway Diagram
Advanced Glycosylation Endproduct Receptor Signaling Pathway antibodies	Advanced Glycosylation Endproduct Receptor Signaling Pathway Diagram
Amyloids Pathway antibodies	Amyloids Pathway Diagram
Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies	Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Cytosolic Sensors Of Pathogen-associated DNA Pathway antibodies	Cytosolic Sensors Of Pathogen-associated DNA Pathway Diagram
DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway antibodies	DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway Diagram
Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies	Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Folate Metabolism Pathway antibodies	Folate Metabolism Pathway Diagram

Diseases associated with anti-SAA1 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>115 publications with SAA1 and Inflammation
Necrosis Antibodies	>35 publications with SAA1 and Necrosis
Kidney Diseases Antibodies	>21 publications with SAA1 and Kidney Diseases
Liver Diseases Antibodies	>21 publications with SAA1 and Liver Diseases
Cardiovascular Diseases Antibodies	>21 publications with SAA1 and Cardiovascular Diseases
Disease Models, Animal Antibodies	>18 publications with SAA1 and Disease Models, Animal
Atherosclerosis Antibodies	>15 publications with SAA1 and Atherosclerosis
Liver Neoplasms Antibodies	>14 publications with SAA1 and Liver Neoplasms
Lung Diseases Antibodies	>13 publications with SAA1 and Lung Diseases
Carcinoma, Hepatocellular Antibodies	>11 publications with SAA1 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-SAA1 antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>85 publications with SAA1 and Liver
Kidney Antibodies	>40 publications with SAA1 and Kidney
Lung Antibodies	>17 publications with SAA1 and Lung
Intestine Antibodies	>14 publications with SAA1 and Intestine
Adipose Tissue Antibodies	>13 publications with SAA1 and Adipose Tissue
Muscle Antibodies	>10 publications with SAA1 and Muscle
Mammary Gland Antibodies	>7 publications with SAA1 and Mammary Gland
Eye Antibodies	>4 publications with SAA1 and Eye
Stomach Antibodies	>3 publications with SAA1 and Stomach
Pancreas Antibodies	>2 publications with SAA1 and Pancreas