Rabbit Anti-RALBP1 Monoclonal Antibody – Cat# MBS179648

Western blot analysis of RALBP1 expression in HeLa cell lysate (MBS179648).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RALBP1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RALBP1

RALBP1 Antibody – Rabbit Monoclonal

Name: RALBP1 Antibody; Monoclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179648
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179648

Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of… More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

RALBP1, Monoclonal Antibody

Full Product Name

Anti-RALBP1 Rabbit Monoclonal Antibody

Product Gene Name

anti-RALBP1 antibody

Product Synonym Names

RalA-binding protein 1; RalBP1; 76 kDa Ral-interacting protein; Dinitrophenyl S-glutathione ATPase; DNP-SG ATPase; Ral-interacting protein 1; RALBP1; RLIP1; RLIP76

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

76063 MW

OMIM

L42542 mRNA

3D Structure

ModBase 3D Structure for Q15311

Immunogen

A synthesized peptide derived from human RALBP1

Subcellular Localization

Membrane; Peripheral membrane protein.

Tissue Specificity

Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-RALBP1 antibody

Cell Biology; Apoptosis; Intracellular; Associated Proteins; Cell Biology; Kinases; Signal Transduction; Adapters; Cytoplasmic; Immunology; Innate Immunity; TLR Signaling

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-RALBP1 antibody

Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB)

Application Notes for anti-RALBP1 antibody

WB: 1:5000-1:10000
IHC: 1:50-1:200
FC/FACS: 1:50

Other Notes

Small volumes of anti-RALBP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RALBP1 purchase

MBS179648 is an antibody targeting RALBP1. Also known as Anti-RALBP1 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RALBP1. It may not necessarily be applicable to this product.

NCBI GI #

5803145

NCBI GeneID

10928

NCBI Accession #

NP_006779.1

UniProt Primary Accession #

Q15311

UniProt Secondary Accession #

D3DUI0

UniProt Related Accession #

Q15311

GenBank Nucleotide #

NM_006788.3

NCBI Official Full Name

ralA-binding protein 1

NCBI Official Synonym Full Names

ralA binding protein 1

NCBI Official Symbol

RALBP1

NCBI Official Synonym Symbols

RIP1; RLIP1; RLIP76

UniProt Protein Name

RalA-binding protein 1

UniProt Synonym Protein Names

76 kDa Ral-interacting protein; Dinitrophenyl S-glutathione ATPase; DNP-SG ATPase; Ral-interacting protein 1

Protein Family

RalA-binding protein

UniProt Gene Name

RALBP1

UniProt Synonym Gene Names

RLIP1; RLIP76; RalBP1; DNP-SG ATPase

NCBI Protein Information

ralA-binding protein 1

NCBI Summary for RALBP1

RALBP1 plays a role in receptor-mediated endocytosis and is a downstream effector of the small GTP-binding protein RAL (see RALA; MIM 179550). Small G proteins, such as RAL, have GDP-bound inactive and GTP-bound active forms, which shift from the inactive to the active state through the action of RALGDS (MIM 601619), which in turn is activated by RAS (see HRAS; MIM 190020) (summary by Feig, 2003 [PubMed 12888294]).[supplied by OMIM, Nov 2010]

Research Articles on RALBP1

1. the miR143-3p level was markedly lower in participants with ovarian cancer compared with normal control, while the expression of RALBP1 mRNA and protein were evidently overexpressed in participants with ovarian cancer compared with normal control.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RALBP1 antibody

Reference Product	PubMed Publications
RALA antibody	>43 publications with RALBP1 and RALA
RALB antibody	>24 publications with RALBP1 and RALB
CDC42 antibody	>16 publications with RALBP1 and CDC42
RAC1 antibody	>12 publications with RALBP1 and RAC1
RHOA antibody	>4 publications with RALBP1 and RHOA
NUMB antibody	>1 publications with RALBP1 and NUMB
RRAS antibody	>1 publications with RALBP1 and RRAS

Pathways associated with anti-RALBP1 antibody

Products by Pathway	Pathway Diagram
EGFR1 Signaling Pathway antibodies	EGFR1 Signaling Pathway Diagram
Pancreatic Cancer Pathway antibodies	Pancreatic Cancer Pathway Diagram
Pancreatic Cancer Pathway antibodies	Pancreatic Cancer Pathway Diagram
Pathways In Cancer antibodies	Pathways In Cancer Diagram
RalA Downstream Regulated Genes Pathway antibodies	RalA Downstream Regulated Genes Pathway Diagram
Ras Signaling Pathway antibodies	Ras Signaling Pathway Diagram
Regulation Of CDC42 Activity Pathway antibodies	Regulation Of CDC42 Activity Pathway Diagram
Regulation Of RAC1 Activity Pathway antibodies	Regulation Of RAC1 Activity Pathway Diagram
Rho GTPase Cycle Pathway antibodies	Rho GTPase Cycle Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram

Diseases associated with anti-RALBP1 antibody

Disease Name	Pubmed Publications
Necrosis Antibodies	>61 publications with RALBP1 and Necrosis
Inflammation Antibodies	>22 publications with RALBP1 and Inflammation
Disease Models, Animal Antibodies	>13 publications with RALBP1 and Disease Models, Animal
Cell Transformation, Neoplastic Antibodies	>12 publications with RALBP1 and Cell Transformation, Neoplastic
Nervous System Diseases Antibodies	>10 publications with RALBP1 and Nervous System Diseases
Adenocarcinoma Antibodies	>9 publications with RALBP1 and Adenocarcinoma
Liver Diseases Antibodies	>8 publications with RALBP1 and Liver Diseases
Poisoning Antibodies	>6 publications with RALBP1 and Poisoning
Urologic Neoplasms Antibodies	>6 publications with RALBP1 and Urologic Neoplasms
Kidney Diseases Antibodies	>5 publications with RALBP1 and Kidney Diseases

Organs/Tissues associated with anti-RALBP1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>37 publications with RALBP1 and Blood
Lung Antibodies	>33 publications with RALBP1 and Lung
Vascular Antibodies	>22 publications with RALBP1 and Vascular
Liver Antibodies	>16 publications with RALBP1 and Liver
Pancreas Antibodies	>11 publications with RALBP1 and Pancreas
Prostate Antibodies	>11 publications with RALBP1 and Prostate
Kidney Antibodies	>11 publications with RALBP1 and Kidney
Brain Antibodies	>10 publications with RALBP1 and Brain
Bone Antibodies	>7 publications with RALBP1 and Bone
Heart Antibodies	>6 publications with RALBP1 and Heart