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CD32 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179660

Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens. More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD32, Monoclonal Antibody
Full Product Name
Anti-CD32 Rabbit Monoclonal Antibody
Product Gene Name
anti-CD32 antibody
Product Synonym Gene Name
FCGR2A
Product Synonym Names
Low affinity immunoglobulin gamma Fc region receptor II-a; IgG Fc receptor II-a; CDw32; Fc-gamma RII-a; Fc-gamma-RIIa; FcRII-a; CD32; FCGR2A; CD32; FCG2; FCGR2A1; IGFR2
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
35001 MW
OMIM
Y00644 mRNA
3D Structure
ModBase 3D Structure for P12318
Immunogen
A synthesized peptide derived from human CD32
Subcellular Localization
Cell membrane; Single-pass type I membrane protein.
Tissue Specificity
Found on monocytes, neutrophils and eosinophil platelets.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-CD32 antibody

Immunology; immunoGlobulins; Receptors; Stem Cells; Hematopoietic Progenitors; Lymphoid; B Lymphocytic Lineage; T Lymphocytic Lineage; Myeloid; monocytic lineage; mast cell lineage
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CD32 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-CD32 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200

Western Blot (WB) of anti-CD32 antibody

Western blot analysis of CD32 expression in K562 cell lysate (MBS179660).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FCGR2A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FCGR2A
Rabbit Anti-CD32 Monoclonal Antibody – Cat# MBS179660

Other Notes

Small volumes of anti-CD32 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD32 purchase

MBS179660 is an antibody targeting CD32. Also known as Anti-CD32 Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CD32. It may not necessarily be applicable to this product.

NCBI GI #
210031822
NCBI GeneID
2212
NCBI Accession #
NP_001129691.1
UniProt Primary Accession #
P12318
UniProt Secondary Accession #
Q8WUN1; Q8WW64
UniProt Related Accession #
P12318
GenBank Nucleotide #
NM_001136219.1
NCBI Official Full Name
low affinity immunoglobulin gamma Fc region receptor II-a isoform 1
NCBI Official Synonym Full Names
Fc fragment of IgG receptor IIa
NCBI Official Symbol
FCGR2A
NCBI Official Synonym Symbols
CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor II-a
UniProt Synonym Protein Names
CDw32; Fc-gamma RII-a; Fc-gamma-RIIa; FcRII-a; CD_antigen: CD32
Protein Family
CD320 antigen
UniProt Gene Name
FCGR2A
UniProt Synonym Gene Names
CD32; FCG2; FCGR2A1; IGFR2; IgG Fc receptor II-a; Fc-gamma-RIIa; FcRII-a
NCBI Protein Information
low affinity immunoglobulin gamma Fc region receptor II-a

NCBI Summary for CD32

This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008]
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Research Articles on CD32

1. FCGR2A rs1801274 G-allele confers susceptibility to Kawasaki disease and Ulcerative colitis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD32 antibody

 Reference Product  PubMed Publications
 CRP antibody  >6 publications with CD32 and CRP
 SYK antibody  >2 publications with CD32 and SYK
 BLK antibody  >2 publications with CD32 and BLK

Pathways associated with anti-CD32 antibody

 Products by Pathway  Pathway Diagram
 FCGR Activation Pathway antibodies  FCGR Activation Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway antibodies  Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Leishmaniasis Pathway antibodies  Leishmaniasis Pathway Diagram
 Leishmaniasis Pathway antibodies  Leishmaniasis Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram

Diseases associated with anti-CD32 antibody

 Disease Name  Pubmed Publications
 Lupus Erythematosus, Systemic Antibodies  >24 publications with CD32 and Lupus Erythematosus, Systemic
 Inflammation Antibodies  >17 publications with CD32 and Inflammation
 Necrosis Antibodies  >14 publications with CD32 and Necrosis
 Arthritis, Rheumatoid Antibodies  >14 publications with CD32 and Arthritis, Rheumatoid
 Thrombocytopenia Antibodies  >8 publications with CD32 and Thrombocytopenia
 Brain Diseases Antibodies  >6 publications with CD32 and Brain Diseases
 Mucocutaneous Lymph Node Syndrome Antibodies  >4 publications with CD32 and Mucocutaneous Lymph Node Syndrome
 Colitis, Ulcerative Antibodies  >4 publications with CD32 and Colitis, Ulcerative
 Thrombosis Antibodies  >3 publications with CD32 and Thrombosis
 Hemorrhage Antibodies  >3 publications with CD32 and Hemorrhage

Organs/Tissues associated with anti-CD32 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >59 publications with CD32 and Blood
 Vascular Antibodies  >6 publications with CD32 and Vascular
 Bone Antibodies  >3 publications with CD32 and Bone
 Kidney Antibodies  >3 publications with CD32 and Kidney
 Skin Antibodies  >2 publications with CD32 and Skin
 Connective Tissue Antibodies  >2 publications with CD32 and Connective Tissue
 Lung Antibodies  >2 publications with CD32 and Lung
 Brain Antibodies  >2 publications with CD32 and Brain
 Stomach Antibodies  >1 publications with CD32 and Stomach
 Eye Antibodies  >1 publications with CD32 and Eye
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