Rabbit Anti-HES1 Monoclonal Antibody – Cat# MBS179662

Western blot analysis of Hes1 expression in SH-SY5Y cell lysate (MBS179662).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HES1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HES1

HES1 Antibody – Rabbit Monoclonal

Name: HES1 Antibody; Monoclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179662
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179662

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Hes1, Monoclonal Antibody

Full Product Name

Anti-Hes1 Rabbit Monoclonal Antibody

Product Gene Name

anti-Hes1 antibody

Product Synonym Names

Transcription factor HES-1; Class B basic helix-loop-helix protein 39; bHLHb39; Hairy and enhancer of split 1; Hairy homolog; Hairy-like protein; hHL; HES1; BHLHB39; HL; HRY

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

29541 MW

OMIM

L19314 Genomic DNA

3D Structure

ModBase 3D Structure for Q14469

Immunogen

A synthesized peptide derived from human Hes1

Subcellular Localization

Nucleus.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-Hes1 antibody

Neuroscience; Neurology Process; Neurogenesis; Epigenetics and Nuclear Signaling; Transcription; Other Factors; Transcription Factors

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-Hes1 antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Application Notes for anti-Hes1 antibody

WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
FC/FACS: 1:50

Other Notes

Small volumes of anti-Hes1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Hes1 purchase

MBS179662 is an antibody targeting Hes1. Also known as Anti-Hes1 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Hes1. It may not necessarily be applicable to this product.

NCBI GI #

5031763

NCBI GeneID

3280

NCBI Accession #

NP_005515.1

UniProt Primary Accession #

Q14469

UniProt Secondary Accession #

Q6FHB2

UniProt Related Accession #

Q14469

GenBank Nucleotide #

NM_005524.3

NCBI Official Full Name

transcription factor HES-1

NCBI Official Synonym Full Names

hes family bHLH transcription factor 1

NCBI Official Symbol

HES1

NCBI Official Synonym Symbols

HHL; HRY; HES-1; bHLHb39

UniProt Protein Name

Transcription factor HES-1

UniProt Synonym Protein Names

Class B basic helix-loop-helix protein 39; bHLHb39; Hairy and enhancer of split 1; Hairy homolog; Hairy-like protein; hHL

Protein Family

Protein

UniProt Gene Name

HES1

UniProt Synonym Gene Names

BHLHB39; HL; HRY; bHLHb39; hHL

NCBI Protein Information

transcription factor HES-1

NCBI Summary for Hes1

This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box. [provided by RefSeq, Jul 2008]

UniProt Comments for Hes1

Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity (). May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage.

Research Articles on Hes1

1. we demonstrate for the first time an essential role of HPV oncoprotein E6 that selectively overexpresses in CaCxSLCs and participates in maintenance of stem cell phenotype and stemness through upregulation of Hes1 while preponderance of E7 leads to differentiation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Hes1 antibody

Reference Product	PubMed Publications
NOTCH1 antibody	>342 publications with Hes1 and NOTCH1
ASCL1 antibody	>52 publications with Hes1 and ASCL1
RBPJ antibody	>47 publications with Hes1 and RBPJ
NEUROG3 antibody	>23 publications with Hes1 and NEUROG3
SHH antibody	>21 publications with Hes1 and SHH
TLE1 antibody	>17 publications with Hes1 and TLE1
FANCD2 antibody	>3 publications with Hes1 and FANCD2
FANCA antibody	>2 publications with Hes1 and FANCA

Pathways associated with anti-Hes1 antibody

Products by Pathway	Pathway Diagram
ATF-2 Transcription Factor Network Pathway antibodies	ATF-2 Transcription Factor Network Pathway Diagram
AhR Pathway antibodies	AhR Pathway Diagram
C-MYB Transcription Factor Network Pathway antibodies	C-MYB Transcription Factor Network Pathway Diagram
Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway antibodies	Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway Diagram
Delta-Notch Signaling Pathway antibodies	Delta-Notch Signaling Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
FBXW7 Mutants And NOTCH1 In Cancer Pathway antibodies	FBXW7 Mutants And NOTCH1 In Cancer Pathway Diagram
Fanconi Anemia Pathway antibodies	Fanconi Anemia Pathway Diagram
Fanconi Anemia Pathway antibodies	Fanconi Anemia Pathway Diagram

Diseases associated with anti-Hes1 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>252 publications with Hes1 and Neoplasms
Carcinoma Antibodies	>85 publications with Hes1 and Carcinoma
Adenocarcinoma Antibodies	>43 publications with Hes1 and Adenocarcinoma
Necrosis Antibodies	>24 publications with Hes1 and Necrosis
Liver Diseases Antibodies	>24 publications with Hes1 and Liver Diseases
Neuroectodermal Tumors, Primitive Antibodies	>22 publications with Hes1 and Neuroectodermal Tumors, Primitive
Lung Neoplasms Antibodies	>22 publications with Hes1 and Lung Neoplasms
Inflammation Antibodies	>21 publications with Hes1 and Inflammation
Carcinoma, Non-Small-Cell Lung Antibodies	>15 publications with Hes1 and Carcinoma, Non-Small-Cell Lung
Breast Neoplasms Antibodies	>15 publications with Hes1 and Breast Neoplasms

Organs/Tissues associated with anti-Hes1 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>221 publications with Hes1 and Brain
Nerve Antibodies	>138 publications with Hes1 and Nerve
Embryonic Tissue Antibodies	>126 publications with Hes1 and Embryonic Tissue
Bone Antibodies	>106 publications with Hes1 and Bone
Blood Antibodies	>102 publications with Hes1 and Blood
Muscle Antibodies	>59 publications with Hes1 and Muscle
Pancreas Antibodies	>56 publications with Hes1 and Pancreas
Liver Antibodies	>54 publications with Hes1 and Liver
Eye Antibodies	>53 publications with Hes1 and Eye
Vascular Antibodies	>49 publications with Hes1 and Vascular