Rabbit Anti-HNRNPA1 Monoclonal Antibody – Cat# MBS179669

Western blot analysis of hnRNP A1 expression in HepG2 cell lysate (MBS179669).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HNRNPA1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HNRNPA1

Hnrnp A1 (HNRNPA1) Antibody – Rabbit Monoclonal

Name: HNRNPA1 Antibody; Monoclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179669
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179669

Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication. More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

hnRNP A1 (HNRNPA1), Monoclonal Antibody

Full Product Name

Anti-hnRNP A1 Rabbit Monoclonal Antibody

Product Gene Name

anti-HNRNPA1 antibody

Product Synonym Names

Heterogeneous nuclear ribonucleoprotein A1; hnRNP A1; Helix-destabilizing protein; Single-strand RNA-binding protein; hnRNP core protein A1; Heterogeneous nuclear ribonucleoprotein A1; N-terminally processed; HNRNPA1; HNRPA1

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

38747 MW

OMIM

X12671 Genomic DNA

3D Structure

ModBase 3D Structure for P09651

Immunogen

A synthesized peptide derived from human hnRNP A1

Subcellular Localization

Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes. In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-HNRNPA1 antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Application Notes for anti-HNRNPA1 antibody

WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
FC/FACS: 1:50

Other Notes

Small volumes of anti-HNRNPA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HNRNPA1 purchase

MBS179669 is an antibody targeting HNRNPA1. Also known as Anti-hnRNP A1 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HNRNPA1. It may not necessarily be applicable to this product.

NCBI GI #

4504445

NCBI GeneID

3178

NCBI Accession #

NP_002127.1

UniProt Primary Accession #

P09651

UniProt Secondary Accession #

Q3MIB7; Q6PJZ7; A8K4Z8

UniProt Related Accession #

P09651

GenBank Nucleotide #

NM_002136.3

NCBI Official Full Name

heterogeneous nuclear ribonucleoprotein A1 isoform a

NCBI Official Synonym Full Names

heterogeneous nuclear ribonucleoprotein A1

NCBI Official Symbol

HNRNPA1

NCBI Official Synonym Symbols

UP 1; ALS19; ALS20; HNRPA1; IBMPFD3; HNRPA1L3; hnRNP A1; hnRNP-A1

UniProt Protein Name

Heterogeneous nuclear ribonucleoprotein A1

UniProt Synonym Protein Names

Helix-destabilizing protein; Single-strand RNA-binding protein; hnRNP core protein A1

Protein Family

Heterogeneous nuclear ribonucleoproteins

UniProt Gene Name

HNRNPA1

UniProt Synonym Gene Names

HNRPA1; hnRNP A1

NCBI Protein Information

heterogeneous nuclear ribonucleoprotein A1

NCBI Summary for HNRNPA1

This gene encodes a member of a family of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs), which are RNA-binding proteins that associate with pre-mRNAs in the nucleus and influence pre-mRNA processing, as well as other aspects of mRNA metabolism and transport. The protein encoded by this gene is one of the most abundant core proteins of hnRNP complexes and plays a key role in the regulation of alternative splicing. Mutations in this gene have been observed in individuals with amyotrophic lateral sclerosis 20. Multiple alternatively spliced transcript variants have been found. There are numerous pseudogenes of this gene distributed throughout the genome. [provided by RefSeq, Feb 2016]

UniProt Comments for HNRNPA1

Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection (PubMed:17371836). May bind to specific miRNA hairpins (PubMed:28431233).

Research Articles on HNRNPA1

1. Case Report: inflammatory myofibroblastic tumor (IMT) of the urinary bladder with a novel HNRNPA1-ALK fusion.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HNRNPA1 antibody

Reference Product	PubMed Publications
XRN1 antibody	>1 publications with HNRNPA1 and XRN1
TNPO1 antibody	>1 publications with HNRNPA1 and TNPO1
SUFU antibody	>1 publications with HNRNPA1 and SUFU
PRMT1 antibody	>1 publications with HNRNPA1 and PRMT1
PABPN1 antibody	>1 publications with HNRNPA1 and PABPN1

Pathways associated with anti-HNRNPA1 antibody

Products by Pathway	Pathway Diagram
Coregulation Of Androgen Receptor Activity Pathway antibodies	Coregulation Of Androgen Receptor Activity Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies	Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
Spliceosome Pathway antibodies	Spliceosome Pathway Diagram
Spliceosome Pathway antibodies	Spliceosome Pathway Diagram
MRNA Splicing Pathway antibodies	MRNA Splicing Pathway Diagram
MRNA Splicing - Major Pathway antibodies	MRNA Splicing - Major Pathway Diagram
MRNA Processing Pathway antibodies	MRNA Processing Pathway Diagram

Diseases associated with anti-HNRNPA1 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>14 publications with HNRNPA1 and Neoplasms
Nervous System Diseases Antibodies	>10 publications with HNRNPA1 and Nervous System Diseases
Brain Diseases Antibodies	>4 publications with HNRNPA1 and Brain Diseases
Carcinoma Antibodies	>3 publications with HNRNPA1 and Carcinoma
Carcinoma, Hepatocellular Antibodies	>2 publications with HNRNPA1 and Carcinoma, Hepatocellular
Drug Toxicity Antibodies	>1 publications with HNRNPA1 and Drug Toxicity
Breast Neoplasms Antibodies	>1 publications with HNRNPA1 and Breast Neoplasms
Kidney Diseases Antibodies	>1 publications with HNRNPA1 and Kidney Diseases
Liver Diseases Antibodies	>1 publications with HNRNPA1 and Liver Diseases
Uterine Cervical Neoplasms Antibodies	>1 publications with HNRNPA1 and Uterine Cervical Neoplasms

Organs/Tissues associated with anti-HNRNPA1 antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>6 publications with HNRNPA1 and Muscle
Brain Antibodies	>5 publications with HNRNPA1 and Brain
Liver Antibodies	>4 publications with HNRNPA1 and Liver
Blood Antibodies	>4 publications with HNRNPA1 and Blood
Kidney Antibodies	>3 publications with HNRNPA1 and Kidney
Heart Antibodies	>3 publications with HNRNPA1 and Heart
Ovary Antibodies	>1 publications with HNRNPA1 and Ovary
Uterus Antibodies	>1 publications with HNRNPA1 and Uterus
Eye Antibodies	>1 publications with HNRNPA1 and Eye
Placenta Antibodies	>1 publications with HNRNPA1 and Placenta