Rabbit Anti-A2M Monoclonal Antibody – Cat# MBS179674

Western blot analysis of A2M expression in human plasma lysate (MBS179674).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-A2M monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for A2M

A2M Antibody – Rabbit Monoclonal

Name: A2M Antibody; Monoclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179674
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179674

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

A2M/Alpha 2 Macroglobulin, Monoclonal Antibody

Full Product Name

Anti-A2M/Alpha 2 Macroglobulin Rabbit Monoclonal Antibody

Product Gene Name

anti-A2M antibody

Product Synonym Names

Alpha-2-macroglobulin; Alpha-2-M; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; A2M; CPAMD5; FWP007

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

163291 MW

OMIM

M11313 mRNA

3D Structure

ModBase 3D Structure for P01023

Immunogen

A synthesized peptide derived from human A2M

Subcellular Localization

Secreted.

Tissue Specificity

Secreted in plasma.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-A2M antibody

Immunology; Innate Immunity; complement; Cell Biology; Proteolysis/Ubiquitin; Protease Inhibitors; Other Protease Inhibitors

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-A2M antibody

Immunohistochemistry (IHC), Western Blot (WB)

Application Notes for anti-A2M antibody

WB: 1:500-1:2000
IHC: 1:50-1:200

Other Notes

Small volumes of anti-A2M antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for A2M purchase

MBS179674 is an antibody targeting A2M. Also known as Anti-A2M/Alpha 2 Macroglobulin Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for A2M. It may not necessarily be applicable to this product.

NCBI GI #

66932947

NCBI GeneID

NCBI Accession #

NP_000005.2

UniProt Primary Accession #

P01023

UniProt Secondary Accession #

Q13677; Q59F47; Q5QTS0; Q68DN2; Q6PIY3; Q6PN97

UniProt Related Accession #

P01023

GenBank Nucleotide #

NM_000014.5

NCBI Official Full Name

alpha-2-macroglobulin isoform a

NCBI Official Synonym Full Names

alpha-2-macroglobulin

NCBI Official Symbol

A2M

NCBI Official Synonym Symbols

A2MD; CPAMD5; FWP007; S863-7

UniProt Protein Name

Alpha-2-macroglobulin

UniProt Synonym Protein Names

C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5

Protein Family

Alpha-2-macroglobulin

UniProt Gene Name

A2M

UniProt Synonym Gene Names

CPAMD5; Alpha-2-M

NCBI Protein Information

alpha-2-macroglobulin

NCBI Summary for A2M

The protein encoded by this gene is a protease inhibitor and cytokine transporter. It uses a bait-and-trap mechanism to inhibit a broad spectrum of proteases, including trypsin, thrombin and collagenase. It can also inhibit inflammatory cytokines, and it thus disrupts inflammatory cascades. Mutations in this gene are a cause of alpha-2-macroglobulin deficiency. This gene is implicated in Alzheimer's disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. A related pseudogene, which is also located on the p arm of chromosome 12, has been identified. [provided by RefSeq, Nov 2016]

UniProt Comments for A2M

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Research Articles on A2M

1. The blood coagulation pathway may be a key regulatory network pathway contributing to the allergic inflammatory response in AR patients. A2M, which is regulated by STAT3, may be an important protein in the pathogenesis of allergic rhinitis in AR patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-A2M antibody

Reference Product	PubMed Publications
APP antibody	>15 publications with A2M and APP
LRP1 antibody	>11 publications with A2M and LRP1
ALB antibody	>6 publications with A2M and ALB
TGFB1 antibody	>2 publications with A2M and TGFB1
FN1 antibody	>1 publications with A2M and FN1
PLG antibody	>1 publications with A2M and PLG

Pathways associated with anti-A2M antibody

Products by Pathway	Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies	Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
HDL-mediated Lipid Transport Pathway antibodies	HDL-mediated Lipid Transport Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
IL6-mediated Signaling Events Pathway antibodies	IL6-mediated Signaling Events Pathway Diagram
Intrinsic Pathway antibodies	Intrinsic Pathway Diagram
Lipid Digestion, Mobilization, And Transport Pathway antibodies	Lipid Digestion, Mobilization, And Transport Pathway Diagram
Lipoprotein Metabolism Pathway antibodies	Lipoprotein Metabolism Pathway Diagram

Diseases associated with anti-A2M antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>89 publications with A2M and Nervous System Diseases
Brain Diseases Antibodies	>85 publications with A2M and Brain Diseases
Mental Disorders Antibodies	>79 publications with A2M and Mental Disorders
Alzheimer Disease Antibodies	>76 publications with A2M and Alzheimer Disease
Neoplasms Antibodies	>31 publications with A2M and Neoplasms
Inflammation Antibodies	>28 publications with A2M and Inflammation
Necrosis Antibodies	>14 publications with A2M and Necrosis
Liver Diseases Antibodies	>12 publications with A2M and Liver Diseases
Fibrosis Antibodies	>11 publications with A2M and Fibrosis
Lung Diseases Antibodies	>11 publications with A2M and Lung Diseases

Organs/Tissues associated with anti-A2M antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>119 publications with A2M and Blood
Liver Antibodies	>35 publications with A2M and Liver
Brain Antibodies	>29 publications with A2M and Brain
Vascular Antibodies	>13 publications with A2M and Vascular
Lung Antibodies	>10 publications with A2M and Lung
Prostate Antibodies	>9 publications with A2M and Prostate
Heart Antibodies	>8 publications with A2M and Heart
Uterus Antibodies	>6 publications with A2M and Uterus
Placenta Antibodies	>5 publications with A2M and Placenta
Embryonic Tissue Antibodies	>5 publications with A2M and Embryonic Tissue