Rabbit Anti-MMP12 Monoclonal Antibody – Cat# MBS179681

Western blot analysis of MMP12 expression in WI-38 cell lysate (MBS179681).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP12 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MMP12

MMP12 Antibody – Rabbit Monoclonal

Name: MMP12 Antibody; Monoclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179681
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179681

May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small… More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MMP12, Monoclonal Antibody

Full Product Name

Anti-MMP12 Rabbit Monoclonal Antibody

Product Gene Name

anti-MMP12 antibody

Product Synonym Names

Macrophage metalloelastase; MME; 3.4.24.65; Macrophage elastase; ME; hME; Matrix metalloproteinase-12; MMP-12; MMP12; HME

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

54002 MW

OMIM

L23808 mRNA

3D Structure

ModBase 3D Structure for P39900

Immunogen

A synthesized peptide derived from human MMP12

Subcellular Localization

Secreted, extracellular space, extracellular matrix.

Tissue Specificity

Found in alveolar macrophages but not in peripheral blood monocytes.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-MMP12 antibody

Cardiovascular; Angiogenesis; Adhesion/ECM; Matrix Metalloproteinases; MMP; Signal Transduction; Cytoskeleton/ECM; Extracellular Matrix; ECM Enzymes; Cancer; Invasion/Microenvironment; MMPS; Tumor Biomarkers; Cardiovascular; Atherosclerosis; Thrombosis

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-MMP12 antibody

Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Application Notes for anti-MMP12 antibody

WB: 1:1000-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:30
FC/FACS: 1:50

Other Notes

Small volumes of anti-MMP12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MMP12 purchase

MBS179681 is an antibody targeting MMP12. Also known as Anti-MMP12 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MMP12. It may not necessarily be applicable to this product.

NCBI GI #

73858572

NCBI GeneID

4321

NCBI Accession #

NP_002417.2

UniProt Primary Accession #

P39900

UniProt Secondary Accession #

Q2M1L9; B2R9X8; B7ZLF6

UniProt Related Accession #

P39900

GenBank Nucleotide #

NM_002426.5

NCBI Official Full Name

macrophage metalloelastase preproprotein

NCBI Official Synonym Full Names

matrix metallopeptidase 12

NCBI Official Symbol

MMP12

NCBI Official Synonym Symbols

ME; HME; MME; MMP-12

UniProt Protein Name

Macrophage metalloelastase

UniProt Synonym Protein Names

Macrophage elastase; ME; hME; Matrix metalloproteinase-12; MMP-12

Protein Family

Macrophage metalloelastase

UniProt Gene Name

MMP12

UniProt Synonym Gene Names

HME; MME; ME; hME; MMP-12

NCBI Protein Information

macrophage metalloelastase

NCBI Summary for MMP12

This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease degrades soluble and insoluble elastin. This gene may play a role in aneurysm formation and mutations in this gene are associated with lung function and chronic obstructive pulmonary disease (COPD). This gene is part of a cluster of MMP genes on chromosome 11. [provided by RefSeq, Jan 2016]

Research Articles on MMP12

1. M4 macrophages are a possible source for HDAC9 and MMP12 expression in advanced human carotid plaques.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MMP12 antibody

Reference Product	PubMed Publications
PLAUR antibody	>2 publications with MMP12 and PLAUR
F12 antibody	>1 publications with MMP12 and F12

Pathways associated with anti-MMP12 antibody

Products by Pathway	Pathway Diagram
Degradation Of Collagen Pathway antibodies	Degradation Of Collagen Pathway Diagram
Degradation Of The Extracellular Matrix Pathway antibodies	Degradation Of The Extracellular Matrix Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Matrix Metalloproteinases Pathway antibodies	Matrix Metalloproteinases Pathway Diagram

Diseases associated with anti-MMP12 antibody

Disease Name	Pubmed Publications
Lung Diseases Antibodies	>81 publications with MMP12 and Lung Diseases
Inflammation Antibodies	>70 publications with MMP12 and Inflammation
Neoplasms Antibodies	>66 publications with MMP12 and Neoplasms
Cardiovascular Diseases Antibodies	>42 publications with MMP12 and Cardiovascular Diseases
Pulmonary Emphysema Antibodies	>35 publications with MMP12 and Pulmonary Emphysema
Disease Models, Animal Antibodies	>32 publications with MMP12 and Disease Models, Animal
Fibrosis Antibodies	>22 publications with MMP12 and Fibrosis
Necrosis Antibodies	>21 publications with MMP12 and Necrosis
Lung Injury Antibodies	>19 publications with MMP12 and Lung Injury
Lung Neoplasms Antibodies	>16 publications with MMP12 and Lung Neoplasms

Organs/Tissues associated with anti-MMP12 antibody

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>95 publications with MMP12 and Lung
Vascular Antibodies	>43 publications with MMP12 and Vascular
Connective Tissue Antibodies	>22 publications with MMP12 and Connective Tissue
Intestine Antibodies	>6 publications with MMP12 and Intestine
Kidney Antibodies	>6 publications with MMP12 and Kidney
Eye Antibodies	>4 publications with MMP12 and Eye
Placenta Antibodies	>4 publications with MMP12 and Placenta
Stomach Antibodies	>3 publications with MMP12 and Stomach
Esophagus Antibodies	>2 publications with MMP12 and Esophagus
Larynx Antibodies	>2 publications with MMP12 and Larynx