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BubR1 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179692

Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
BubR1, Monoclonal Antibody
Full Product Name
Anti-BubR1 Rabbit Monoclonal Antibody
Product Gene Name
anti-BubR1 antibody
Product Synonym Gene Name
BUB1B
Product Synonym Names
Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; 2.7.11.1; MAD3/BUB1-related protein kinase; hBUBR1; Mitotic checkpoint kinase MAD3L; Protein SSK1; BUB1B; BUBR1; MAD3L; SSK1
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
119545 MW
OMIM
AF053306 mRNA
3D Structure
ModBase 3D Structure for O60566
Immunogen
A synthesized peptide derived from human BubR1
Subcellular Localization
Cytoplasm. Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic in interphase cells. Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and CASC5.
Tissue Specificity
Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-BubR1 antibody

Cell Biology; Cell Cycle; Cell Division; spindle; Epigenetics and Nuclear Signaling; Kinases/Phosphatases; Cancer
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-BubR1 antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-BubR1 antibody
WB: 1:1000-1:5000
IHC: 1:50-1:200
IP: 1:30
FC/FACS: 1:50

Western Blot (WB) of anti-BubR1 antibody

Western blot analysis of BubR1 expression in HepG2 cell lysate (MBS179692).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BUB1B monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BUB1B
Rabbit Anti-BubR1 Monoclonal Antibody – Cat# MBS179692

Other Notes

Small volumes of anti-BubR1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BubR1 purchase

MBS179692 is an antibody targeting BubR1. Also known as Anti-BubR1 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BubR1. It may not necessarily be applicable to this product.

NCBI GI #
59814247
NCBI GeneID
701
NCBI Accession #
NP_001202.4
UniProt Primary Accession #
O60566
UniProt Secondary Accession #
O60501; O60627; O60758; O75389; Q59HH6; Q8WV50; Q96KM4; B2R6U0; B4DL09; B4DLG3
UniProt Related Accession #
O60566
GenBank Nucleotide #
NM_001211.5
NCBI Official Full Name
mitotic checkpoint serine/threonine-protein kinase BUB1 beta
NCBI Official Synonym Full Names
BUB1 mitotic checkpoint serine/threonine kinase B
NCBI Official Symbol
BUB1B
NCBI Official Synonym Symbols
MVA1; SSK1; BUBR1; Bub1A; MAD3L; hBUBR1; BUB1beta
UniProt Protein Name
Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
UniProt Synonym Protein Names
MAD3/BUB1-related protein kinase; hBUBR1; Mitotic checkpoint kinase MAD3L; Protein SSK1
Protein Family
Mitotic spindle checkpoint protein
UniProt Gene Name
BUB1B
UniProt Synonym Gene Names
BUBR1; MAD3L; SSK1; hBUBR1
NCBI Protein Information
mitotic checkpoint serine/threonine-protein kinase BUB1 beta

NCBI Summary for BubR1

This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. [provided by RefSeq, Jul 2008]
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Research Articles on BubR1

1. Data show that BRCA2 was required for HDAC2/3 association with acetylated BubR1 in nocodazole (Noc)-arrested cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BubR1 antibody

 Reference Product  PubMed Publications
 BUB3 antibody  >6 publications with BubR1 and BUB3
 CDC20 antibody  >6 publications with BubR1 and CDC20
 CENPE antibody  >3 publications with BubR1 and CENPE
 BRCA2 antibody  >2 publications with BubR1 and BRCA2

Pathways associated with anti-BubR1 antibody

 Products by Pathway  Pathway Diagram
 APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies  APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram

Diseases associated with anti-BubR1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >35 publications with BubR1 and Neoplasms
 Carcinoma Antibodies  >11 publications with BubR1 and Carcinoma
 Breast Neoplasms Antibodies  >8 publications with BubR1 and Breast Neoplasms
 Colorectal Neoplasms Antibodies  >8 publications with BubR1 and Colorectal Neoplasms
 Adenocarcinoma Antibodies  >7 publications with BubR1 and Adenocarcinoma
 Microcephaly Antibodies  >5 publications with BubR1 and Microcephaly
 Neoplasms, Experimental Antibodies  >3 publications with BubR1 and Neoplasms, Experimental
 Carcinoma, Hepatocellular Antibodies  >3 publications with BubR1 and Carcinoma, Hepatocellular
 Neoplasm Metastasis Antibodies  >3 publications with BubR1 and Neoplasm Metastasis
 Rhabdomyosarcoma, Embryonal Antibodies  >2 publications with BubR1 and Rhabdomyosarcoma, Embryonal

Organs/Tissues associated with anti-BubR1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >5 publications with BubR1 and Blood
 Intestine Antibodies  >2 publications with BubR1 and Intestine
 Brain Antibodies  >2 publications with BubR1 and Brain
 Bone Antibodies  >2 publications with BubR1 and Bone
 Lymph Node Antibodies  >1 publications with BubR1 and Lymph Node
 Lymph Antibodies  >1 publications with BubR1 and Lymph
 Thymus Antibodies  >1 publications with BubR1 and Thymus
 Mouth Antibodies  >1 publications with BubR1 and Mouth
 Ovary Antibodies  >1 publications with BubR1 and Ovary
 Stomach Antibodies  >1 publications with BubR1 and Stomach
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