Rabbit Anti-ELANE Monoclonal Antibody – Cat# MBS179704

Western blot analysis of Neutrophil Elastase expression in HL60 cell lysate (MBS179704).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ELANE monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ELANE

Neutrophil Elastase (ELANE) Antibody – Rabbit Monoclonal

Name: ELANE Antibody; Monoclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179704
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179704

Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Neutrophil Elastase (ELANE), Monoclonal Antibody

Full Product Name

Anti-Neutrophil Elastase Rabbit Monoclonal Antibody

Product Gene Name

anti-ELANE antibody

Product Synonym Names

Neutrophil elastase; 3.4.21.37; Bone marrow serine protease; Elastase-2; Human leukocyte elastase; HLE; Medullasin; PMN elastase; ELANE; ELA2

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

28518 MW

OMIM

Y00477 Genomic DNA

3D Structure

ModBase 3D Structure for P08246

Immunogen

A synthesized peptide derived from human Neutrophil Elastase

Tissue Specificity

Bone marrow cells.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-ELANE antibody

Immunology; Innate Immunity; Macrophage/Inflamm.; Immunology; complement; Regulatory; Microbiology; Protein; Human Protein; Defensin; Signal Transduction; Cytoskeleton/ECM; Extracellular Matrix; ECM Proteins; elastin; Signaling Pathway; Calcium Signaling

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-ELANE antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Application Notes for anti-ELANE antibody

WB: 1:500-1:2000
IHC: 1:100-1:500
ICC/IF: 1:50-1:200
FC/FACS: 1:30

Other Notes

Small volumes of anti-ELANE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ELANE purchase

MBS179704 is an antibody targeting ELANE. Also known as Anti-Neutrophil Elastase Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ELANE. It may not necessarily be applicable to this product.

NCBI GI #

4503549

NCBI GeneID

1991

NCBI Accession #

NP_001963.1

UniProt Primary Accession #

P08246

UniProt Secondary Accession #

P09649; Q6B0D9; Q6LDP5

UniProt Related Accession #

P08246

GenBank Nucleotide #

NM_001972.3

NCBI Official Full Name

neutrophil elastase preproprotein

NCBI Official Synonym Full Names

elastase, neutrophil expressed

NCBI Official Symbol

ELANE

NCBI Official Synonym Symbols

GE; NE; HLE; HNE; ELA2; SCN1; PMN-E

UniProt Protein Name

Neutrophil elastase

UniProt Synonym Protein Names

Bone marrow serine protease; Elastase-2; Human leukocyte elastase; HLE; Medullasin; PMN elastase

Protein Family

Neutrophil elastase

UniProt Gene Name

ELANE

UniProt Synonym Gene Names

ELA2; HLE

NCBI Protein Information

neutrophil elastase

NCBI Summary for ELANE

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19. [provided by RefSeq, Jan 2016]

Research Articles on ELANE

1. Using different blood leukocyte and skin resident cell preparations, and recombinant proteins, the authors have identified that neutrophil elastase, but not other neutrophil derived proteases, cleaves IL-36Ra into its highly active antagonistic form.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ELANE antibody

Reference Product	PubMed Publications
MPO antibody	>1 publications with ELANE and MPO

Pathways associated with anti-ELANE antibody

Products by Pathway	Pathway Diagram
Activation Of Matrix Metalloproteinases Pathway antibodies	Activation Of Matrix Metalloproteinases Pathway Diagram
C-MYB Transcription Factor Network Pathway antibodies	C-MYB Transcription Factor Network Pathway Diagram
Degradation Of Collagen Pathway antibodies	Degradation Of Collagen Pathway Diagram
Degradation Of The Extracellular Matrix Pathway antibodies	Degradation Of The Extracellular Matrix Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Systemic Lupus Erythematosus Pathway antibodies	Systemic Lupus Erythematosus Pathway Diagram
Systemic Lupus Erythematosus Pathway antibodies	Systemic Lupus Erythematosus Pathway Diagram
Transcriptional Misregulation In Cancer Pathway antibodies	Transcriptional Misregulation In Cancer Pathway Diagram
Transcriptional Misregulation In Cancer Pathway antibodies	Transcriptional Misregulation In Cancer Pathway Diagram
Amb2 Integrin Signaling Pathway antibodies	Amb2 Integrin Signaling Pathway Diagram

Diseases associated with anti-ELANE antibody

Disease Name	Pubmed Publications
Neutropenia Antibodies	>31 publications with ELANE and Neutropenia
Cyclic neutropenia Antibodies	>7 publications with ELANE and Cyclic neutropenia
NEUTROPENIA, SEVERE CONGENITAL, 1, AUTOSOMAL DOMINANT Antibodies	>5 publications with ELANE and NEUTROPENIA, SEVERE CONGENITAL, 1, AUTOSOMAL DOMINANT
Lung Diseases Antibodies	>3 publications with ELANE and Lung Diseases
Disease Models, Animal Antibodies	>2 publications with ELANE and Disease Models, Animal
Inflammation Antibodies	>2 publications with ELANE and Inflammation
Shock Antibodies	>1 publications with ELANE and Shock
Liver Diseases Antibodies	>1 publications with ELANE and Liver Diseases
Pain Antibodies	>1 publications with ELANE and Pain
Weight Loss Antibodies	>1 publications with ELANE and Weight Loss

Organs/Tissues associated with anti-ELANE antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>18 publications with ELANE and Blood
Bone Marrow Antibodies	>5 publications with ELANE and Bone Marrow
Lung Antibodies	>3 publications with ELANE and Lung