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RAD18 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179707

E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap- filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the… More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Rad18, Monoclonal Antibody
Full Product Name
Anti-Rad18 Rabbit Monoclonal Antibody
Product Gene Name
anti-Rad18 antibody
Product Synonym Names
E3 ubiquitin-protein ligase RAD18; 6.3.2.-; Postreplication repair protein RAD18; hHR18; hRAD18; RING finger protein 73; RAD18; RNF73
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
56223 MW
OMIM
AB035274 mRNA
3D Structure
ModBase 3D Structure for Q9NS91
Immunogen
A synthesized peptide derived from human Rad18
Subcellular Localization
Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Associates with chromatin (PubMed: 25931565). Colocalizes with SLF1 in the nucleus and to centrosomes (PubMed: 15632077). Relocalizes with SLF1 to nuclear foci in response to DNA damage (PubMed: 22036607). Accumulates with the SLF1-SLF2 and SMC5-SMC6 complexes at replication-coupled DNA interstrand repair and DNA double-strand breaks (DSBs) sites on chromatin in a ubiquitin-dependent manner (PubMed: 25931565).
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-Rad18 antibody

Epigenetics and Nuclear Signaling; DNA/RNA; dna Synthesis; translesion Synthesis; Cell Biology; Proteolysis/Ubiquitin; Proteasome/Ubiquitin; ubiquitin e3 Enzymes; ring finger e3 ligase; Epigenetics and Nuclear Signaling; ChIP'ing Antibodies
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-Rad18 antibody
Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes for anti-Rad18 antibody
WB: 1:10000-1:20000
FC/FACS: 1:20

Western Blot (WB) of anti-Rad18 antibody

Western blot analysis of Rad18 expression in HeLa cell lysate (MBS179707).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAD18 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RAD18
Rabbit Anti-RAD18 Monoclonal Antibody – Cat# MBS179707

Other Notes

Small volumes of anti-Rad18 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Rad18 purchase

MBS179707 is an antibody targeting Rad18. Also known as Anti-Rad18 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Rad18. It may not necessarily be applicable to this product.

NCBI GI #
256818821
NCBI GeneID
56852
NCBI Accession #
NP_064550.3
UniProt Primary Accession #
Q9NS91
UniProt Secondary Accession #
Q58F55; Q9NRT6
UniProt Related Accession #
Q9NS91
GenBank Nucleotide #
NM_020165.3
NCBI Official Full Name
E3 ubiquitin-protein ligase RAD18
NCBI Official Synonym Full Names
RAD18, E3 ubiquitin protein ligase
NCBI Official Symbol
RAD18
NCBI Official Synonym Symbols
RNF73
UniProt Protein Name
E3 ubiquitin-protein ligase RAD18
UniProt Synonym Protein Names
Postreplication repair protein RAD18; hHR18; hRAD18; RING finger protein 73; RING-type E3 ubiquitin transferase RAD18Curated
Protein Family
E3 ubiquitin-protein ligase
UniProt Gene Name
RAD18
UniProt Synonym Gene Names
RNF73; hHR18; hRAD18
NCBI Protein Information
E3 ubiquitin-protein ligase RAD18

NCBI Summary for Rad18

The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008]
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UniProt Comments for Rad18

E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.
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Research Articles on Rad18

1. Data suggest that, as part of DNA repair in nucleus of embryonic stem cells, IGF1R interacts with and phosphorylates PCNA at tyrosine residues 60, 133, and 250; this is followed by mono- and polyubiquitination of PCNA by RAD18 and SHPRH. (IGF1R = insulin-like growth factor 1 receptor; PCNA = proliferating cell nuclear antigen; RAD18 = E3 ubiquitin-protein ligase RAD18; SHPRH = E3 ubiquitin-protein ligase SHPRH)
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Rad18 antibody

 Reference Product  PubMed Publications
 UBE2A antibody  >8 publications with Rad18 and UBE2A
 UBE2B antibody  >7 publications with Rad18 and UBE2B

Diseases associated with anti-Rad18 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >14 publications with Rad18 and Neoplasms
 Skin Neoplasms Antibodies  >3 publications with Rad18 and Skin Neoplasms
 Carcinoma, Squamous Cell Antibodies  >3 publications with Rad18 and Carcinoma, Squamous Cell
 Lung Neoplasms Antibodies  >2 publications with Rad18 and Lung Neoplasms
 Precancerous Conditions Antibodies  >2 publications with Rad18 and Precancerous Conditions
 Weight Loss Antibodies  >2 publications with Rad18 and Weight Loss
 Nervous System Diseases Antibodies  >2 publications with Rad18 and Nervous System Diseases
 Cell Transformation, Neoplastic Antibodies  >1 publications with Rad18 and Cell Transformation, Neoplastic
 Breast Neoplasms Antibodies  >1 publications with Rad18 and Breast Neoplasms
 Inflammation Antibodies  >1 publications with Rad18 and Inflammation

Organs/Tissues associated with anti-Rad18 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >9 publications with Rad18 and Testis
 Skin Antibodies  >6 publications with Rad18 and Skin
 Blood Antibodies  >5 publications with Rad18 and Blood
 Lung Antibodies  >4 publications with Rad18 and Lung
 Bone Antibodies  >3 publications with Rad18 and Bone
 Liver Antibodies  >2 publications with Rad18 and Liver
 Bone Marrow Antibodies  >2 publications with Rad18 and Bone Marrow
 Ovary Antibodies  >2 publications with Rad18 and Ovary
 Thymus Antibodies  >2 publications with Rad18 and Thymus
 Brain Antibodies  >2 publications with Rad18 and Brain
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