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Troponin I (TNNI3) Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179725

Anti-Troponin I Rabbit Monoclonal Antibody MBS179725. Tested in Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB) applications. This antibody reacts with Human. More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Troponin I (TNNI3), Monoclonal Antibody
Full Product Name
Anti-Troponin I Rabbit Monoclonal Antibody
Product Gene Name
Product Synonym Names
Troponin I; cardiac muscle; Cardiac troponin I; TNNI3; TNNC1
Clonality
Monoclonal
Clone Number
EFD-20
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/mL BSA.
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20°C for one year. For short term and frequent use, store at 4°C for one month. Avoid repeated freezing and thawing.
OMIM
X54163 mRNA
3D Structure
ModBase 3D Structure for P19429
Immunogen
A synthesized peptide derived from human Troponin I
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-TNNI3 antibody
Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-TNNI3 antibody
Dilute the sample so that the expected range of concentrations fall within the detection range of this kit.
If the expected range of concentration is unknown, a pilot test should be conducted to decide the optimal dilution ratio for your samples.
Some PubMed article(s) citing the expression level of this target are as follows:
QC testing used:
WB 1:5000-1:10000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50

Western Blot (WB) of anti-TNNI3 antibody

Western blot analysis of Troponin I expression in fetal heart cell lysate (MBS179725).
Rabbit Anti-TNNI3 Monoclonal Antibody – Cat# MBS179725

Other Notes

Small volumes of anti-TNNI3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TNNI3 purchase

MBS179725 is an antibody targeting TNNI3. Also known as Anti-Troponin I Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TNNI3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
troponin I, cardiac muscle
NCBI Official Synonym Full Names
troponin I3, cardiac type
NCBI Official Symbol
TNNI3
NCBI Official Synonym Symbols
CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF
UniProt Protein Name
Troponin I, cardiac muscle
UniProt Synonym Protein Names
Cardiac troponin I
Protein Family
UniProt Gene Name
TNNI3
UniProt Synonym Gene Names
TNNC1
NCBI Protein Information
troponin I, cardiac muscle

NCBI Summary for TNNI3

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]
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UniProt Comments for TNNI3

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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