Rabbit Anti-RAD21 Monoclonal Antibody – Cat# MBS179755

Western blot analysis of Rad21 expression in HeLa cell lysate (MBS179755).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAD21 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RAD21

RAD21 Antibody – Rabbit Monoclonal

Name: RAD21 Antibody; Monoclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179755
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179755

Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a… More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Rad21, Monoclonal Antibody

Full Product Name

Anti-Rad21 Rabbit Monoclonal Antibody

Product Gene Name

anti-Rad21 antibody

Product Synonym Names

Double-strand-break repair protein rad21 homolog; hHR21; Nuclear matrix protein 1; NXP-1; SCC1 homolog; RAD21; HR21; KIAA0078; NXP1

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

71690 MW

OMIM

X98294 mRNA

3D Structure

ModBase 3D Structure for O60216

Immunogen

A synthesized peptide derived from human Rad21

Subcellular Localization

Nucleus. Chromosome. Chromosome, centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, it is cleaved by separase/ESPL1, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. Once cleaved by caspase-3, the C-terminal 64 kDa cleavage product translocates to the cytoplasm, where it may trigger apoptosis.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-Rad21 antibody

Cell Biology; Cell Cycle; Cell Division; chromatid cohesion; Epigenetics and Nuclear Signaling; DNA/RNA; DNA Damage & Repair; DNA Damage Response; Chromosome Structure; cohesins; Cancer; ChIP'ing Antibodies

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-Rad21 antibody

Flow Cytometry (FC/FACS), Western Blot (WB)

Application Notes for anti-Rad21 antibody

WB: 1:500-1:1000
FC/FACS: 1:50

Other Notes

Small volumes of anti-Rad21 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Rad21 purchase

MBS179755 is an antibody targeting Rad21. Also known as Anti-Rad21 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Rad21. It may not necessarily be applicable to this product.

NCBI GI #

5453994

NCBI GeneID

5885

NCBI Accession #

NP_006256.1

UniProt Primary Accession #

O60216

UniProt Secondary Accession #

Q15001; Q99568; A8K0E0

UniProt Related Accession #

O60216

GenBank Nucleotide #

NM_006265.2

NCBI Official Full Name

double-strand-break repair protein rad21 homolog

NCBI Official Synonym Full Names

RAD21 cohesin complex component

NCBI Official Symbol

RAD21

NCBI Official Synonym Symbols

HR21; MCD1; NXP1; SCC1; CDLS4; hHR21; HRAD21

UniProt Protein Name

Double-strand-break repair protein rad21 homolog

UniProt Synonym Protein Names

Nuclear matrix protein 1; NXP-1; SCC1 homolog

Protein Family

Cofilin

UniProt Gene Name

RAD21

UniProt Synonym Gene Names

; hHR21; NXP-1

NCBI Protein Information

double-strand-break repair protein rad21 homolog

NCBI Summary for Rad21

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq, Jul 2008]

Research Articles on Rad21

1. This study together with previous reports suggests incomplete penetrance associated with RAD21 variants and these individuals may therefore be underdiagnosed.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Rad21 antibody

Reference Product	PubMed Publications
SMC3 antibody	>92 publications with Rad21 and SMC3
SMC1A antibody	>34 publications with Rad21 and SMC1A
NIPBL antibody	>34 publications with Rad21 and NIPBL
STAG2 antibody	>13 publications with Rad21 and STAG2
ESPL1 antibody	>12 publications with Rad21 and ESPL1
WAPAL antibody	>7 publications with Rad21 and WAPAL
STAG1 antibody	>7 publications with Rad21 and STAG1
PDS5B antibody	>4 publications with Rad21 and PDS5B
CDCA5 antibody	>3 publications with Rad21 and CDCA5
PDS5A antibody	>2 publications with Rad21 and PDS5A

Pathways associated with anti-Rad21 antibody

Products by Pathway	Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cohesin Loading Onto Chromatin Pathway antibodies	Cohesin Loading Onto Chromatin Pathway Diagram
Establishment Of Sister Chromatid Cohesion Pathway antibodies	Establishment Of Sister Chromatid Cohesion Pathway Diagram
M Phase Pathway antibodies	M Phase Pathway Diagram
Meiosis Pathway antibodies	Meiosis Pathway Diagram
Meiotic Synapsis Pathway antibodies	Meiotic Synapsis Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram

Diseases associated with anti-Rad21 antibody

Disease Name	Pubmed Publications
Leukemia, Myeloid Antibodies	>12 publications with Rad21 and Leukemia, Myeloid
Neoplasm Metastasis Antibodies	>6 publications with Rad21 and Neoplasm Metastasis
Neoplasms, Experimental Antibodies	>4 publications with Rad21 and Neoplasms, Experimental
Ovarian Neoplasms Antibodies	>3 publications with Rad21 and Ovarian Neoplasms
Infertility, Male Antibodies	>3 publications with Rad21 and Infertility, Male
Necrosis Antibodies	>3 publications with Rad21 and Necrosis
Adenocarcinoma Antibodies	>3 publications with Rad21 and Adenocarcinoma
Liver Neoplasms Antibodies	>2 publications with Rad21 and Liver Neoplasms
Cell Transformation, Neoplastic Antibodies	>2 publications with Rad21 and Cell Transformation, Neoplastic
Fibrosis Antibodies	>2 publications with Rad21 and Fibrosis

Organs/Tissues associated with anti-Rad21 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>23 publications with Rad21 and Blood
Testis Antibodies	>13 publications with Rad21 and Testis
Bone Antibodies	>10 publications with Rad21 and Bone
Brain Antibodies	>9 publications with Rad21 and Brain
Ovary Antibodies	>6 publications with Rad21 and Ovary
Bone Marrow Antibodies	>6 publications with Rad21 and Bone Marrow
Liver Antibodies	>5 publications with Rad21 and Liver
Lymph Node Antibodies	>5 publications with Rad21 and Lymph Node
Lymph Antibodies	>5 publications with Rad21 and Lymph
Embryonic Tissue Antibodies	>4 publications with Rad21 and Embryonic Tissue