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S100P Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179776

May function as calcium sensor and contribute to cellular calcium signaling. In a calcium-dependent manner, functions by interacting with other proteins, such as EZR and PPP5C, and indirectly plays a role in physiological processes like the formation of… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
S100P, Monoclonal Antibody
Full Product Name
Anti-S100P Rabbit Monoclonal Antibody
Product Gene Name
anti-S100P antibody
Product Synonym Names
Protein S100-P; Migration-inducing gene 9 protein; MIG9; Protein S100-E; S100 calcium-binding protein P; S100P; S100E
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
10400 MW
OMIM
X65614 mRNA
3D Structure
ModBase 3D Structure for P25815
Immunogen
A synthesized peptide derived from human S100P
Subcellular Localization
Nucleus. Cytoplasm. Cell projection, microvillus membrane. Colocalizes with S100PBP in the nucleus. Colocolizes with EZR in the microvilli in a calcium- dependent manner.
Tissue Specificity
Detected in all of the tissues except brain, testis and small intestine, expression level is higher in placenta, heart, lung, skeletal muscle, spleen and leukocyte. Up- regulated in various pancreatic ductal adenocarcinomas and pancreatic intraepithelial neoplasias.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-S100P antibody

Signal Transduction; Signaling Pathway; Calcium Signaling; Calcium Binding Proteins; Cancer; Tumor Biomarkers
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-S100P antibody
Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-S100P antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50

Western Blot (WB) of anti-S100P antibody

Western blot analysis of S100P expression in SW480 cell lysate (MBS179776).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100P monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for S100P
Rabbit Anti-S100P Monoclonal Antibody – Cat# MBS179776

Other Notes

Small volumes of anti-S100P antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for S100P purchase

MBS179776 is an antibody targeting S100P. Also known as Anti-S100P Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for S100P. It may not necessarily be applicable to this product.

NCBI GI #
5174663
NCBI GeneID
6286
NCBI Accession #
NP_005971.1
UniProt Primary Accession #
P25815
UniProt Secondary Accession #
Q5J7W2
UniProt Related Accession #
P25815
GenBank Nucleotide #
NM_005980.2
NCBI Official Full Name
protein S100-P
NCBI Official Synonym Full Names
S100 calcium binding protein P
NCBI Official Symbol
S100P
NCBI Official Synonym Symbols
MIG9
UniProt Protein Name
Protein S100-P
UniProt Synonym Protein Names
Migration-inducing gene 9 protein; MIG9; Protein S100-E; S100 calcium-binding protein P
Protein Family
Protein
UniProt Gene Name
S100P
UniProt Synonym Gene Names
S100E; MIG9
NCBI Protein Information
protein S100-P

NCBI Summary for S100P

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 4p16. This protein, in addition to binding Ca2+, also binds Zn2+ and Mg2+. This protein may play a role in the etiology of prostate cancer. [provided by RefSeq, Jul 2008]
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Research Articles on S100P

1. the S100P protein may contribute to the outgrowth of aggressive tumor cells resistant to cytotoxic therapy and promote cancer progression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-S100P antibody

 Reference Product  PubMed Publications
 S100A4 antibody  >30 publications with S100P and S100A4
 S100A2 antibody  >20 publications with S100P and S100A2
 RAGE antibody  >20 publications with S100P and RAGE
 S100A1 antibody  >16 publications with S100P and S100A1
 S100PBP antibody  >7 publications with S100P and S100PBP
 S100A3 antibody  >3 publications with S100P and S100A3
 MYH9 antibody  >1 publications with S100P and MYH9

Pathways associated with anti-S100P antibody

 Products by Pathway  Pathway Diagram
 Gastric Cancer Network 1 Pathway antibodies  Gastric Cancer Network 1 Pathway Diagram

Diseases associated with anti-S100P antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >101 publications with S100P and Carcinoma
 Adenocarcinoma Antibodies  >77 publications with S100P and Adenocarcinoma
 Neoplasm Metastasis Antibodies  >45 publications with S100P and Neoplasm Metastasis
 Pancreatic Neoplasms Antibodies  >42 publications with S100P and Pancreatic Neoplasms
 Breast Neoplasms Antibodies  >29 publications with S100P and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >16 publications with S100P and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >15 publications with S100P and Lung Neoplasms
 Carcinoma, Squamous Cell Antibodies  >15 publications with S100P and Carcinoma, Squamous Cell
 Prostatic Neoplasms Antibodies  >14 publications with S100P and Prostatic Neoplasms
 Cell Transformation, Neoplastic Antibodies  >14 publications with S100P and Cell Transformation, Neoplastic

Organs/Tissues associated with anti-S100P antibody

 Organ/Tissue Name  Pubmed Publications
 Prostate Antibodies  >26 publications with S100P and Prostate
 Pancreas Antibodies  >25 publications with S100P and Pancreas
 Liver Antibodies  >21 publications with S100P and Liver
 Lung Antibodies  >20 publications with S100P and Lung
 Blood Antibodies  >15 publications with S100P and Blood
 Lymph Node Antibodies  >13 publications with S100P and Lymph Node
 Stomach Antibodies  >13 publications with S100P and Stomach
 Bladder Antibodies  >11 publications with S100P and Bladder
 Placenta Antibodies  >10 publications with S100P and Placenta
 Uterus Antibodies  >7 publications with S100P and Uterus
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