Rabbit Anti-MLANA Monoclonal Antibody – Cat# MBS179788

Western blot analysis of MLANA expression in Human melanoma tissue lysate (MBS179788).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLANA monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MLANA

MLANA Antibody – Rabbit Monoclonal

Name: MLANA Antibody; Monoclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179788
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179788

Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes. More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MLANA/Mart 1, Monoclonal Antibody

Full Product Name

Anti-MLANA/Mart 1 Rabbit Monoclonal Antibody

Product Gene Name

anti-MLANA antibody

Product Synonym Names

Melanoma antigen recognized by T-cells 1; MART-1; Antigen LB39-AA; Antigen SK29-AA; Protein Melan-A; MLANA; MART1

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

13157 MW

OMIM

U06452 mRNA

3D Structure

ModBase 3D Structure for Q16655

Immunogen

A synthesized peptide derived from human MLANA

Subcellular Localization

Endoplasmic reticulum membrane; Single-pass type III membrane protein. Golgi apparatus. Golgi apparatus, trans-Golgi network membrane. Melanosome. Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post- Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation.

Tissue Specificity

Expression is restricted to melanoma and melanocyte cell lines and retina.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-MLANA antibody

Tags & Cell Markers; Cell Type Markers; Tumor Associated; Cancer; Tumor Immunology; tumor-associated antigens

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-MLANA antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Application Notes for anti-MLANA antibody

WB: 1:1000-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
FC/FACS: 1:200

Other Notes

Small volumes of anti-MLANA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MLANA purchase

MBS179788 is an antibody targeting MLANA. Also known as Anti-MLANA/Mart 1 Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MLANA. It may not necessarily be applicable to this product.

NCBI GI #

5031913

NCBI GeneID

2315

NCBI Accession #

NP_005502.1

UniProt Primary Accession #

Q16655

UniProt Secondary Accession #

Q6ICU4

UniProt Related Accession #

Q16655

GenBank Nucleotide #

NM_005511.1

NCBI Official Full Name

melanoma antigen recognized by T-cells 1

NCBI Official Synonym Full Names

melan-A

NCBI Official Symbol

MLANA

NCBI Official Synonym Symbols

MART1; MART-1

UniProt Protein Name

Melanoma antigen recognized by T-cells 1

UniProt Synonym Protein Names

Antigen LB39-AA; Antigen SK29-AA; Protein Melan-A

Protein Family

Melanoma antigen recognized by T-cells

UniProt Gene Name

MLANA

UniProt Synonym Gene Names

MART1; MART-1

NCBI Protein Information

melanoma antigen recognized by T-cells 1

Research Articles on MLANA

1. Melan-A-stained slides shows definitive invasion.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-MLANA antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>935 publications with MLANA and Neoplasms
Skin Neoplasms Antibodies	>292 publications with MLANA and Skin Neoplasms
Carcinoma Antibodies	>77 publications with MLANA and Carcinoma
Neoplasms, Experimental Antibodies	>63 publications with MLANA and Neoplasms, Experimental
Necrosis Antibodies	>48 publications with MLANA and Necrosis
Adenocarcinoma Antibodies	>34 publications with MLANA and Adenocarcinoma
Nervous System Diseases Antibodies	>32 publications with MLANA and Nervous System Diseases
Liver Neoplasms Antibodies	>27 publications with MLANA and Liver Neoplasms
Kidney Diseases Antibodies	>26 publications with MLANA and Kidney Diseases
Lung Neoplasms Antibodies	>21 publications with MLANA and Lung Neoplasms

Organs/Tissues associated with anti-MLANA antibody

Organ/Tissue Name	Pubmed Publications
Skin Antibodies	>348 publications with MLANA and Skin
Eye Antibodies	>33 publications with MLANA and Eye
Kidney Antibodies	>31 publications with MLANA and Kidney
Brain Antibodies	>21 publications with MLANA and Brain
Ear Antibodies	>7 publications with MLANA and Ear
Embryonic Tissue Antibodies	>5 publications with MLANA and Embryonic Tissue
Mammary Gland Antibodies	>1 publications with MLANA and Mammary Gland