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JAM1 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179794

Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction… More details

$210 / 0.03 mL +1 FREE 8GB USB
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
JAM1, Monoclonal Antibody
Full Product Name
Anti-JAM1 Rabbit Monoclonal Antibody
Product Gene Name
anti-JAM1 antibody
Product Synonym Gene Name
F11R
Product Synonym Names
Junctional adhesion molecule A; JAM-A; Junctional adhesion molecule 1; JAM-1; Platelet F11 receptor; Platelet adhesion molecule 1; PAM-1; CD321; F11R; JAM1; JCAM; UNQ264/PRO301
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
32583 MW
OMIM
AF111713 mRNA
3D Structure
ModBase 3D Structure for Q9Y624
Immunogen
A synthesized peptide derived from human JAM1
Subcellular Localization
Cell junction, tight junction. Cell membrane; Single-pass type I membrane protein. Localized at tight junctions of both epithelial and endothelial cells.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-JAM1 antibody

Cardiovascular; Blood; Platelets; Signal Transduction; Cytoskeleton/ECM; Cell Adhesion; Tight Junctions; Cardiovascular; Atherosclerosis; Vascular Inflammation; Leukocyte Recruitment; Cell Junction Molecules
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-JAM1 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-JAM1 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200

Western Blot (WB) of anti-JAM1 antibody

Western blot analysis of JAM1 expression in HeLa cell lysate (MBS179794).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-F11R monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for F11R
Rabbit Anti-JAM1 Monoclonal Antibody – Cat# MBS179794

Other Notes

Small volumes of anti-JAM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for JAM1 purchase

MBS179794 is an antibody targeting JAM1. Also known as Anti-JAM1 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for JAM1. It may not necessarily be applicable to this product.

NCBI GI #
1129371461
NCBI GeneID
50848
NCBI Accession #
NP_001335020.1
UniProt Primary Accession #
Q9Y624
UniProt Secondary Accession #
B7Z941
UniProt Related Accession #
Q9Y624
GenBank Nucleotide #
NM_001348091.1
NCBI Official Full Name
junctional adhesion molecule A isoform 6
NCBI Official Synonym Full Names
F11 receptor
NCBI Official Symbol
F11R
NCBI Official Synonym Symbols
JAM; KAT; JAM1; JAMA; JCAM; CD321; PAM-1
UniProt Protein Name
Junctional adhesion molecule A
UniProt Synonym Protein Names
Junctional adhesion molecule 1; JAM-1; Platelet F11 receptor; Platelet adhesion molecule 1; PAM-1; CD_antigen: CD321
UniProt Gene Name
F11R
UniProt Synonym Gene Names
JAM1; JCAM; JAM-A; JAM-1; PAM-1
NCBI Protein Information
junctional adhesion molecule A

NCBI Summary for JAM1

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established. [provided by RefSeq, Jul 2008]
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UniProt Comments for JAM1

Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3 (PubMed:11489913). The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier (). Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration (PubMed:11812992). Involved in platelet activation (PubMed:10753840).
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Research Articles on JAM1

1. Dysregulation of JAM-A via p63/GATA-3 signaling pathway occurs in squamous cell carcinomas of the head and neck.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-JAM1 antibody

 Reference Product  PubMed Publications
 TJP1 antibody  >27 publications with JAM1 and TJP1
 CLDN1 antibody  >4 publications with JAM1 and CLDN1
 MLLT4 antibody  >2 publications with JAM1 and MLLT4
 ITGB2 antibody  >1 publications with JAM1 and ITGB2
 MPDZ antibody  >1 publications with JAM1 and MPDZ

Pathways associated with anti-JAM1 antibody

 Products by Pathway  Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Junction Organization Pathway antibodies  Cell Junction Organization Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Cell-Cell Communication Pathway antibodies  Cell-Cell Communication Pathway Diagram
 Cell-cell Junction Organization Pathway antibodies  Cell-cell Junction Organization Pathway Diagram
 Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway antibodies  Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway Diagram
 Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway antibodies  Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram

Diseases associated with anti-JAM1 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >37 publications with JAM1 and Inflammation
 Breast Neoplasms Antibodies  >17 publications with JAM1 and Breast Neoplasms
 Carcinoma Antibodies  >14 publications with JAM1 and Carcinoma
 Disease Models, Animal Antibodies  >13 publications with JAM1 and Disease Models, Animal
 Necrosis Antibodies  >13 publications with JAM1 and Necrosis
 Heart Diseases Antibodies  >8 publications with JAM1 and Heart Diseases
 Neoplasm Metastasis Antibodies  >8 publications with JAM1 and Neoplasm Metastasis
 Adenocarcinoma Antibodies  >7 publications with JAM1 and Adenocarcinoma
 Neoplasm Invasiveness Antibodies  >7 publications with JAM1 and Neoplasm Invasiveness
 Lung Neoplasms Antibodies  >5 publications with JAM1 and Lung Neoplasms

Organs/Tissues associated with anti-JAM1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >79 publications with JAM1 and Blood
 Vascular Antibodies  >54 publications with JAM1 and Vascular
 Heart Antibodies  >19 publications with JAM1 and Heart
 Intestine Antibodies  >18 publications with JAM1 and Intestine
 Lung Antibodies  >16 publications with JAM1 and Lung
 Brain Antibodies  >15 publications with JAM1 and Brain
 Liver Antibodies  >13 publications with JAM1 and Liver
 Kidney Antibodies  >9 publications with JAM1 and Kidney
 Embryonic Tissue Antibodies  >9 publications with JAM1 and Embryonic Tissue
 Lymph Antibodies  >7 publications with JAM1 and Lymph
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