Rabbit Anti-MFN1 Monoclonal Antibody – Cat# MBS179805

Western blot analysis of Mitofusin1 expression in K562 cell lysate (MBS179805).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MFN1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MFN1

Mitofusin1 (MFN1) Antibody – Rabbit Monoclonal

Name: MFN1 Antibody; Monoclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179805
Price: 485 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179805

Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Mitofusin1 (MFN1), Monoclonal Antibody

Full Product Name

Anti-Mitofusin1 Rabbit Monoclonal Antibody

Product Gene Name

anti-MFN1 antibody

Product Synonym Names

Mitofusin-1; 3.6.5.-; Fzo homolog; Transmembrane GTPase MFN1; MFN1

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

84100 MW

OMIM

AF329637 mRNA

3D Structure

ModBase 3D Structure for Q8IWA4

Immunogen

A synthesized peptide derived from human Mitofusin1

Subcellular Localization

Mitochondrion outer membrane; Multi- pass membrane protein.

Tissue Specificity

Ubiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-MFN1 antibody

Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)

Application Notes for anti-MFN1 antibody

WB: 1:1000-1:5000
IP: 1:20
FC/FACS: 1:20

Other Notes

Small volumes of anti-MFN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MFN1 purchase

MBS179805 is an antibody targeting MFN1. Also known as Anti-Mitofusin1 Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MFN1. It may not necessarily be applicable to this product.

NCBI GI #

45269137

NCBI GeneID

55669

NCBI Accession #

NP_284941.2

UniProt Primary Accession #

Q8IWA4

UniProt Secondary Accession #

O15323; O60639; Q9BZB5; Q9NWQ2; B2RAR1; D3DNR6

UniProt Related Accession #

Q8IWA4

GenBank Nucleotide #

NM_033540.2

NCBI Official Full Name

mitofusin-1

NCBI Official Synonym Full Names

mitofusin 1

NCBI Official Symbol

MFN1

NCBI Official Synonym Symbols

hfzo1; hfzo2

UniProt Protein Name

Mitofusin-1

UniProt Synonym Protein Names

Fzo homolog

Protein Family

Mitofusin

UniProt Gene Name

MFN1

NCBI Protein Information

mitofusin-1

NCBI Summary for MFN1

The protein encoded by this gene is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting. [provided by RefSeq, Jul 2008]

UniProt Comments for MFN1

Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion (PubMed:12475957, PubMed:12759376, PubMed:27920125, PubMed:28114303). Membrane clustering requires GTPase activity (PubMed:27920125). It may involve a major rearrangement of the coiled coil domains (PubMed:27920125, PubMed:28114303). Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events (PubMed:12475957, PubMed:12759376). Overexpression induces the formation of mitochondrial networks (in vitro) (PubMed:12759376). Has low GTPase activity (PubMed:27920125, PubMed:28114303).

Research Articles on MFN1

1. Regulation of Mfn1 by MGRN1 and the proteasome modulates mitochondrial fusion.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MFN1 antibody

Reference Product	PubMed Publications
FIS1 antibody	>47 publications with MFN1 and FIS1
DNM1L antibody	>39 publications with MFN1 and DNM1L
PARK2 antibody	>6 publications with MFN1 and PARK2
MARCH5 antibody	>5 publications with MFN1 and MARCH5
GDAP1 antibody	>4 publications with MFN1 and GDAP1
BAK1 antibody	>3 publications with MFN1 and BAK1
MAVS antibody	>3 publications with MFN1 and MAVS
PHB2 antibody	>1 publications with MFN1 and PHB2

Pathways associated with anti-MFN1 antibody

Products by Pathway	Pathway Diagram
Factors Involved In Megakaryocyte Development And Platelet Production Pathway antibodies	Factors Involved In Megakaryocyte Development And Platelet Production Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram

Diseases associated with anti-MFN1 antibody

Disease Name	Pubmed Publications
Mitochondrial Diseases Antibodies	>40 publications with MFN1 and Mitochondrial Diseases
Heart Diseases Antibodies	>12 publications with MFN1 and Heart Diseases
Disease Models, Animal Antibodies	>10 publications with MFN1 and Disease Models, Animal
Nerve Degeneration Antibodies	>8 publications with MFN1 and Nerve Degeneration
Inflammation Antibodies	>6 publications with MFN1 and Inflammation
Kidney Diseases Antibodies	>3 publications with MFN1 and Kidney Diseases
Hypertrophy Antibodies	>3 publications with MFN1 and Hypertrophy
Cardiomyopathies Antibodies	>3 publications with MFN1 and Cardiomyopathies
Weight Loss Antibodies	>3 publications with MFN1 and Weight Loss
Poisoning Antibodies	>3 publications with MFN1 and Poisoning

Organs/Tissues associated with anti-MFN1 antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>41 publications with MFN1 and Muscle
Brain Antibodies	>38 publications with MFN1 and Brain
Heart Antibodies	>28 publications with MFN1 and Heart
Nerve Antibodies	>15 publications with MFN1 and Nerve
Blood Antibodies	>9 publications with MFN1 and Blood
Embryonic Tissue Antibodies	>6 publications with MFN1 and Embryonic Tissue
Kidney Antibodies	>5 publications with MFN1 and Kidney
Lung Antibodies	>4 publications with MFN1 and Lung
Bone Antibodies	>4 publications with MFN1 and Bone
Liver Antibodies	>4 publications with MFN1 and Liver